A comparative study of antibacterial effects of mouthwashes containing Ag/ZnO or ZnO nanoparticles with chlorhexidine and investigation of their cytotoxicity

Objective(s): Chlorhexidine 0.2% mouthwash is commonly used in orthodontic patients for plaque control. But it has some side effects. Metal oxide nanoparticles have been recently used in mouthwashes in reports. So we aimed to evaluate antibacterial effect of ZnO and Ag/ZnO nanoparticles against Streptococcus mutans and compare them with chlorhexidine 0.2%, sodium fluoride 0.05% and some of their compositions. Materials and Methods: ZnO and Ag/ZnO NPs were synthesized and sixteen groups of mouthwashes were prepared. We used Zone of Inhibition (ZOI) test to evaluation of antibacterial effects of as-prepared mouthwashes, against S. mutans. The cytotoxicity of the ZnO and Ag/ZnO NPs were investigated in the A549 cell line.Results: Among the study groups, the maximum ZOI (16.60±0.49 mm) pertained to Ag/ZnO, 10 mg NPs plus 100 ml base material, (Ag/ZnO b 10). The results indicate that no significant harmful effect is imposed to the cells up to 0.2 mg/ml of ZnO and Ag/ZnO NPs.Conclusion: Results showed that mouthwash containing Ag/ZnO b 10 has the highest antibacterial properties against S .mutans amoung study groups and because in this concentration it is safe for cells, so it can be served as an alternative mouthwash in plaque control instead of chlorhexidine 0.2% after in vivo studies

Comparing Vitamin D Serum Levels in Patients with Oral Lichen Planus and Healthy Subjects

Statement of the Problem: Lichen planus disease is a chronic inflammatory lesion without a known etiology. Recent studies have indicated the role of vitamin D on immune system and proposed its anti-inflammatory effects. Purpose: This study aimed to compare vitamin D serum levels in patients with oral lichen planus and healthy subjects. Materials and Method: In this case‒control study, 18 patients suffering from oral lichen planus referred to the Department of Oral Medicine, Tabriz Faculty of Dentistry were chosen as the case group and 18 healthy people were chosen as the control group. A 5-mL blood sample was taken from all subjects and the subjects’ vitamin D serum levels were assessed with a vitamin D total (25-hydroxy vitamin D) kit by employing the electrochemiluminescence technique. The results were analyzed and compared by using SPSS17 statistic software. p< 0.05 was considered statistically significant. Results: The mean vitamin D level in serum of patients with oral lichen planus was 30.7±20.38ng/ml and in healthy subjects was 36.45± 15.33ng/ml, the difference was not statistically significant (p= 0.346). Conclusion: The difference between the level of vitamin D in the serum of patients suffering from oral lichen planus and healthy individuals was not significant

The relationship between the average score of first two years and comprehensive basic science examination score of dentistry students of Tabriz University of medical sciences

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