Kolaviron attenuates diclofenac-induced nephrotoxicity in male Wistar rats

The beneficial effects of kolaviron, a natural biflavonoid from the seeds of Garcinia kola, have been attributed to its antioxidant and anti-inflammatory activities. This study was designed to investigate the renoprotective effect of kolaviron in rat model of diclofenac (DFC)-induced acute renal failure. Thirty-five male Wistar rats were divided into seven groups of five rats each as follows: a control group that received propylene glycol orally and treatment groups that received diclofenac, diclofenac followed by kolaviron at three different doses and kolaviron only. Diclofenac treated rats showed sluggishness, illness and anorexia. Their urine contained appreciable protein, glucose and ketone bodies. Histopathological examination of their kidneys revealed profound acute tubular necrosis. Diclofenac treatment significantly increased levels of plasma creatinine, urea, sodium, chloride, potassium ions, and increased renal tissue activities of superoxide dismutase, catalase, levels of malondialdehyde and hydrogen peroxide. Fractional excretion of sodium and potassium and renal tissue levels of reduced glutathione and prostaglandin E2 (PGE2) decreased significantly in DFC treated groups. However, kolaviron administration significantly reduced toxic effect of DFC on PGE2 release, plasma levels of creatinine, urea, glucose, and electrolytes and significantly attenuated renal tubular and oxidative damages. Furthermore, the effects of DFC administration on food consumption, water intake, urine output and urine protein, glucose, ketone bodies and electrolytes, were significantly attenuated in animals treated with kolaviron. The results suggested that kolaviron ameliorated DFC-induced kidney injury in Wistar rats by decreasing renal oxidative damage and restoration of renal PGE2 release back to the basal levels.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author