Cancer Gene Therapy to Restore P53 Function: A New Way for an Old Aim

Millions of people are living with cancer having specific mutation in p53 gene while every single person is truly unique in genetic basis or clinical manifestation. The gene encodes transcription factor p53, which plays a central role in regulating cell cycle progression, senescence, differentiation, DNA repair and apoptosis in response to DNA damage or other stress signals. P53 activity is up regulated to initiate a cascade of biological events that ultimately results in prevention of tumor development. Mutations in p53 abrogate normal tumor suppressor functions, contributing to the survival and proliferation of abnormal cells. Cancer cells containing mutant p53 are associated with more aggressive disease, increased resistance to chemotherapy and radiation therapy, and poor prognosis. However the majority of p53 mutations are missense and great number of these mutants represent GOF (Gain of Function) effect resulting increased invasion and metastasis in tumors. These mutations confer a dominant-negative activity over the remaining wild-type allele by functionally inactive hetero-oligomers interactions of the mutants with the wild-type protein. Increasing evidence indicates that many p53 mutants also gain new oncogenic properties that are independent from wild-type p53. Several factors including type of p53 mutations in cancers may limit the efficacy and application of p53 gene therapy. As a result, there is a great interest in therapeutic strategies aimed at restoring the function of p53 for the treatment of cancer. Increasing evidence demonstrate that silencing GOF mutations (targeted antisense therapy) reduce the transactivation activity of mutant p53 and induce apoptosis in cells bearing these mutations then provide a potential strategy to inhibit the oncogenic functions of mutant p53 and improve mutant p53-targeted cancer therapies

The effect of self-scrutiny on improving women’s self-esteem: A case study of Esfahan women

The proposed study of this paper investigates the effect of self-scrutiny on improving women’s self-esteem among 50 women who live in city of Esfahan, Iran. There are five hypotheses associated with the proposed study of this paper, which investigate whether teaching self-scrutiny skills increase women’s total, educational-vocational, general, social and family self-esteem. The proposed study of this paper uses three groups of pre-test post-test and control groups and it has been executed among 50 women aged 20-35 who lived in city of Esfahan, Iran based on Coopersmith questionnaire. The results of ANOVA test confirm all five hypotheses of this survey. In other words, scrutiny skills increase women’s total, educational-vocational, general, social and family self-esteem after control group participate in our training programs

The Role of Helicobacter Pylori Eradication in the Treatment of Chronic Urticaria in Comparison with Classic Treatments

Introduction: Helicobacter pylori (H.pylori) is the most common chronic worldwide bacterial infection in humans in all of ages and  the majority of Chronic Urticarial patients also carry     H. Pylori. The purpose of the present study was to studied H. pylori eradication in the treatment of chronic urticaria in comparison with classic treatment on two treatment groups. Materials & Methods: participants were 120 Chronic Urticaria patients with H. Pylori infected and positive Urease Breath Test (UBT) who refer to Quaem Hospital in Mashhad. Participants were randomly assigned two groups, control group received classic treatment of Urticaria (H1 & H2 Blockers) and investigation group was additional treatment by triple drug therapy (20 mg of Robeprazole twice daily, 500 mg of Clarithromycin twice daily, and 1gr of Amoxicillin twice daily during 14 days). Within one, three & six months after therapy symptoms were separately analyzed in two groups by using urticaria severity score. Results: As to the age factor, no significant difference was found (P-value: 0.863). However, the average age with regard to gender, was significantly higher in men (P-value: 0.006).The majority of patients who had been exposed to combination drug treatment, were significantly better cured than the group exposed to classic treatment lonely (P-value < 0.001) and many components of urticaria severity score showed better improvement respectively (P-value < 0.001). Conclusions: Addition of this treatment for eradication of Helicobacter pylori infection in patients with chronic urticaria, could be more effective than the classic H1and H2 blockers alone for resolution of symptoms