ASSESSMENT OF THE RESPIRATORY SYMPTOMS AMONG THE WORKERS OF CEMENT FACTORY

Background: Even in the twenty-first century, millions of people are working daily in a dusty environment. They are exposed to different types of health hazards i.e. fume, gases and dust, which are risk factors in developing occupational disease. Cement industry is involved in the development of structure of this advanced and modern world but generates dust during its production. Cement dust causes lung function impairment, chronic obstructive lung disease, restrictive lung disease, pneumoconiosis and carcinoma of the lungs, stomach and colon. Objective: To assess the respiratory symptoms among the workers of cement factory. Methodology: A cross sectional study was conducted in Cement factory. Data and peak expiratory rate was collected from 100 factory workers regarding their respiratory symptoms. The data was analyzed on SPSS version 18. Conclusion: Total cement dust exposure was related to acute respiratory symptoms and acute ventilatory effects. Implementing measures to control dust and providing adequate personal respiratory protective equipment for the Production workers are highly recommended Keywords: Portland cement dust, exposure, Lung function impairmen

Investigating metal (M = Mn, Fe, and Ni)-doped Co(OH) 2 nanofibers for electrocatalytic oxygen evolution and electrochemical biosensing performance

To achieve efficient and cost-effective electrochemical water splitting, highly active and affordable nanostructured catalysts are the key requirement. The current study presents the investigations of the efficacy of metal (Mn, Fe and Ni)-doped Co(OH)2 nanofibers towards oxygen evolution via water splitting. Notably, Ni-doped Co(OH)2 demonstrates superior OER performance in KOH electrolyte, surpassing standard IrO2 with a modest potential of 1.62 V at 10 mA cm−2. The remarkable activity is attributed to the nanofiber structure, facilitating faster conduction and offering readily available active sites. Ni-doped Co(OH)2 nanofibers displayed enduring stability even after 1000 cycles. This work underscores the importance of transition-metal based catalysts as effective electrocatalysts, providing the groundwork for the development of cutting-edge catalysts. Additionally, the electrochemical sensing capability towards ascorbic acid is evaluated, with Ni-doped Co(OH)2 showing the most promising response, characterized by the lowest LOD and LOQ values. These findings highlight the potential of Ni-doped Co(OH)2 nanofibers for upcoming diagnostic detection devices

Investigating metal (M = Mn, Fe, and Ni)-doped Co(OH) 2 nanofibers for electrocatalytic oxygen evolution and electrochemical biosensing performance

To achieve efficient and cost-effective electrochemical water splitting, highly active and affordable nanostructured catalysts are the key requirement. The current study presents the investigations of the efficacy of metal (Mn, Fe and Ni)-doped Co(OH)2 nanofibers towards oxygen evolution via water splitting. Notably, Ni-doped Co(OH)2 demonstrates superior OER performance in KOH electrolyte, surpassing standard IrO2 with a modest potential of 1.62 V at 10 mA cm−2. The remarkable activity is attributed to the nanofiber structure, facilitating faster conduction and offering readily available active sites. Ni-doped Co(OH)2 nanofibers displayed enduring stability even after 1000 cycles. This work underscores the importance of transition-metal based catalysts as effective electrocatalysts, providing the groundwork for the development of cutting-edge catalysts. Additionally, the electrochemical sensing capability towards ascorbic acid is evaluated, with Ni-doped Co(OH)2 showing the most promising response, characterized by the lowest LOD and LOQ values. These findings highlight the potential of Ni-doped Co(OH)2 nanofibers for upcoming diagnostic detection devices

Climate Smart Crops for Food Security

After ensuring the food security for over 50 years, the green revolution is eventually reaching its biological limits which are very much reflected by the ongoing stagnancy in yield increased over the past few decades. Meeting the increasing food demands due to increasing population is the greatest challenge for today’s plant scientists. Changing climatic conditions are posing additional threats to crop growth, productivity and yield. After successfully deploying gene editing to modify simple traits, scientists are now embarked on more ambitious adventures in genomics to combat challenges of food security in the wake of increasing population and climate change adversaries. The chapter outlines use of new technologies in tailoring crops beyond simple traits aiming to harvest the desired diversity lost during domestication and manipulating complex traits, which evolved over evolutionary timescale with special emphasis on the development of climate smart crops

shRNA-mediated gene silencing of HDAC11 empowers CAR-T cells against prostate cancer

Epigenetic mechanisms are involved in several cellular functions, and their role in the immune system is of prime importance. Histone deacetylases (HDACs) are an important set of enzymes that regulate and catalyze the deacetylation process. HDACs have been proven beneficial targets for improving the efficacy of immunotherapies. HDAC11 is an enzyme involved in the negative regulation of T cell functions. Here, we investigated the potential of HDAC11 downregulation using RNA interference in CAR-T cells to improve immunotherapeutic outcomes against prostate cancer. We designed and tested four distinct short hairpin RNA (shRNA) sequences targeting HDAC11 to identify the most effective one for subsequent analyses. HDAC11-deficient CAR-T cells (shD-NKG2D-CAR-T) displayed better cytotoxicity than wild-type CAR-T cells against prostate cancer cell lines. This effect was attributed to enhanced activation, degranulation, and cytokine release ability of shD-NKG2D-CAR-T when co-cultured with prostate cancer cell lines. Our findings reveal that HDAC11 interference significantly enhances CAR-T cell proliferation, diminishes exhaustion markers PD-1 and TIM3, and promotes the formation of T central memory TCM populations. Further exploration into the underlying molecular mechanisms reveals increased expression of transcription factor Eomes, providing insight into the regulation of CAR-T cell differentiation. Finally, the shD-NKG2D-CAR-T cells provided efficient tumor control leading to improved survival of tumor-bearing mice in vivo as compared to their wild-type counterparts. The current study highlights the potential of HDAC11 downregulation in improving CAR-T cell therapy. The study will pave the way for further investigations focused on understanding and exploiting epigenetic mechanisms for immunotherapeutic outcomes

Surgery for complicated pulmonary tuberculosis

Abstract not availabl

Reconnoitering the nexus between organizational culture and open innovation systems

Postmodern organizations strive to cultivate open innovation systems to foster product innovation and commercialization. Cultural dynamics can be a massive obstacle in flourishing the inbound and outbound open innovation in many organizations. However, little is known about the effect of different types of culture on the open innovation dimensions. This research aimed to investigate the role of cultural subtleties in nurturing inbound and outbound open innovation in the organizations. A quantitative research design was employed, and data were collected from a random sample of 231 managers from the automotive industry. Structural Equation Modeling (SEM) was used to test the hypotheses using Smart PLS. The results indicated that hierarchy culture, associated with formal rules and regulations, negatively affects the inbound and outbound open innovation. The Market culture has a positive relationship with inbound and outbound open innovation while adhocracy culture could positively impact the inbound open innovation only. On the other hand, clan culture has a negative effect on the outbound open innovation. We concluded that the organizational culture focused on internal integrations is more suitable for outbound open innovation, and the type of organizational culture focused on external differentiation is more likely to support inbound open innovation

Graves’ Disease: Pathophysiology, Genetics and Management

Graves’ disease is an autoimmune disorder in which hyperthyroidism (over active thyroid) is caused by the autoantibodies against the TSH receptor. It is mainly characterized by the appearance of goiter. The symptoms are wide ranging as thyroid hormone affects many body systems. It is common in women and in people with age below than 40. Graves’ disease is caused by a combination of genetic and environmental factors while genetics being the main cause. Graves’ disease is not a single gene defect but has a complex pattern of inheritance. Today it is clear that genetic predisposition to Graves’ disease is caused by multiple genes. HLA gene is one the most studied gene predisposing to Graves’ disease. Lot of polymorphisms in this gene has been to be associated with the disease. Lymphoid tyrosine phosphatase encoded by the gene PTPN22 has been found to increase the risk of many autoimmune diseases including Graves’ disease. The best documented association of PTPN22 variants to autoimmune disorders including GD is rs2476601 (C1858T). Other genes associated with the risk of GD are thyrotropin receptor (TSHR), thyroglobulin gene, FCRL3, SCGB3A2, and CTLA4. This chapter will discuss in detail the genetics, pathophysiology, diagnosis and treatment of Graves’ hyperthyroidism

Efficacy of the muscle energy technique versus the strain-counter strain technique on immediate deactivation of myofascial trigger points in upper trapezius muscle

Aims and objectives: Thisstudy aimed to check the comparative efficacy of the Muscle EnergyTechnique versus the Strain-Counter Strain technique on immediate deactivation ofmyofascial trigger points in the upper trapezius muscle. Study design: The study comprised acomparative analytical design to compare and contrast the two study interventions. Place andduration of the study: The research was conducted in the Department of Physical therapy,Allied hospital Faisalabad for 6 months. Patients and methods: Based on inclusion andexclusion criteria, 40 subjects were enrolled in the study using the convenient samplingmethod. The subjects were divided into two groups; group A (n=20) received a single sessionof baseline treatment with themuscle energy technique, while group B (n=20)received a singlesession of baseline treatment with the strain-counter strain technique. The subjects wereevaluated through the pressure-pain threshold (algometer), Numeric Pain Rating Scale, andModified Bournemouth Questionnaire as pre-intervention and post-intervention measuringtools for pain and functional status. Results: The data was analyzed using SPSS version 17.Within group analysis showed a significant difference between pre- values and post values ofpressure-pain threshold, Numeric Pain Rating Scale and Modified BournemouthQuestionnaire in both groups (P<0.05). Between group analysis was done using independentsample t test. It also showed significant difference (P<0.05) in post mean values between thetwo group subjects in all three outcomes. The post-mean values for the strain-counter straingroup were slightly more improved than the Muscle Energy Technique group. Conclusion:The strain-counter strain technique is found more effective than the Muscle Energy Techniquefor immediate deactivation of myofascial trigger points in the upper trapezius muscle