A computer model of family building based on expected values; abstract of an article by Potter and Sakoda

Includes bibliographyPublicado en Demography, vol. 3(2), 196

Estimación de la fecundidad mediante métodos propuestos por Brass

Incluye Bibliografí

Guatemala, censo experimental de 1970: aplicacion de las tecnicas del profesor William Brass para estimar la fecundidad y la mortalidad

Incluye Bibliografí

Chile: censo de una poblacion marginal; analisis demografico

Incluye Bibliografí

Buenos Aires, Bogota y San Jose: aplicacion del metodo de Brass para la medicion de la fecundidad y la mortalidad

Incluye Bibliografí

Buenos Aires, Bogota y San Jose: aplicacion del metodo de Brass para la medicion de la fecundidad y la mortalidad

Incluye Bibliografí

Highly protective E2-CSFV vaccine candidate produced in the mammary gland of adenoviral transduced goats

Classical swine fever virus is the etiological agent of the most economically important highly contagious disease of swine worldwide. E2 is the major envelope glycoprotein present as a homodimer on the outer surface of the virus and represents an important target for the induction of neutralizing immune response against the viral infection. The E2 extracellular domain was expressed in the milk of adenoviral transduced goats at the highest level about 1.2 g/L. The recombinant glycoprotein was purified from clarified serum milk by a single metal chelate affinity chromatography step, as a homodimer of approximately 100 kDa and purity over 98%. Glycosylation analysis showed the presence of oligomannoside, hybrid and complex type N-glycans, attached to the recombinant E2. The capacity of goat milk-derived E2 antigen to protect pigs from both classical swine fever clinical signs and viral infection was assessed in a vaccination and challenge trial. The immunized pigs became protected after challenge with 105 LD50 of a highly pathogenic CSFV strain. In the context of veterinary vaccines, this expression system has the advantages that the recombinant antigen could be harvested in about 48 h after adenoviral transduction with expression levels in the range of g/L. This approach may turn into a scalable expression system for the assessment and production of veterinary vaccines. © 2007 Elsevier B.V. All rights reserved