2 research outputs found
Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings
Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings
Safflower (Carthamus tinctorius L.) is an important oilseed crop, usually
grown on a small scale and in salt-affected soils. Salinity stress can cause
oxidative damage to plants. Upregulation of the antioxidant defense system
induced by glycine betaine (GlyBet) alleviates the damaging effects of
oxidative stress in plants. In the present investigation, seeds were treated
with 0, 10, 30 and 60 mM of GlyBet solutions. Germination and the primary
growth of the seedling were examined using sodium chloride salt (NaCl) at 0
(non-stress), 50, 100 and 150 mM concentrations. The obtained results
indicate that at 50 and 100 mM NaCl, priming with 30 and 60 mM GlyBet
increased root and shoot lengths compared to the control (0 mM). In addition,
at all stress levels, priming with 60 mM GlyBet led to lower malondialdehyde,
total soluble sugars and proline contents than in control seedlings. Priming
with GlyBet increased catalase (CAT), superoxide dismutase (SOD) enzyme
activities and protein content, while it reduced the activity of peroxidase
under salinity stress. In addition, priming with GlyBet reduced the Na+/K+
ratio of seedlings and increased K+ under all salinity stress levels. Priming
with 60 mM GlyBet also reduced the Na+ content under 150 mM NaCl. Together,
these results show that 60 mM GlyBet had the most pronounced effect on
tolerance to salinity stress in safflower seedling. The glycine
betaine-increased tolerance to salt in safflower was mainly related to
increased CAT and SOD activities, and the prevention of cell membrane damage
as a result of reduced lipid peroxidation and improved ion homeostasis under
salinity stress condition