Antibacterial Activity of Some Household Surface Cleaners against Common Pathogens

Surface cleaner has been widely used to control infections and its transmission. In Hospitals Antiseptics are used to control microbial growth on living tissues and inanimate objects. The activity of six commercial household surface cleaner, Dettol, Phenyl, Harpic, Max, sweepy and local surface cleaner were tested against common pathogens of 10 different species of each strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Bacillus subtilis. Disc diffusion method were implanted with different concentrations of 100%,75%,50%,25% for each surface cleaner and applied on MHA. After incubation of 24 hours inhibition zones were measured as the maximum zone of inhibition were observed by Staphylococcus aureus at 75 % concentration and the minimum zone of inhibition were observed by Bacillus subtilis at 25 % concentrations. This conducted study showed effectively killing of Disinfectant in all test organisms and provide greatest protection in the transmission of diseases

Bacteriostatic Activity of Lemon Juice against Staphylococcal Skin Infections

Antimicrobial herbs are center of attraction from a very long time back. These herbs can cure variety of the infections including GIT, liver and skin infections. In this conducted study we have analyzed lemon as an antibacterial herb against skin infections. A total of 25 skin lesion samples were collected out of which 14 were Staphylococcus epidermidis and 06 were Staphylococcus aureus were isolated. By pure culture study method all the species of Staphylococci gives bacteriostatic growth by the activity of lemon juice which was analyzed by Agar well diffusion method. Some other antimicrobial herb are also efficient against skin infections like aloe vera, neem etc. Because of the presence of Vitamin C lemon juices aids some more health effects to the skin and have less side effects than antibiotics

Diarrhea Outbreak Caused by Contaminated Water used for Vegetables Sale

Water has always played a prominent role in human civilization. The water sources used for supplying water were not always clean, and coliforms are the major source of diarrheal infections especially in developing countries and hence contaminated water is the main reason for the spread of these coliforms with in a community by different ways. The contamination of water used in vegetables by pathogens cause diarrheal disease is the most important aspect of vegetables quality. This problem arises as a consequence of contamination of water by faecal matter, particularly human faecal matter, containing pathogenic organisms. Vegetable sellers usually use dirty/contaminated water to shower on vegetables to keep them fresh. This study describes the outbreaks of diarrheal diseases along with the reasons for the outbreaks. In our study we have used Membrane filter technique to test the quality of water used by the vegetable sellers. Purpose of this study is to detect the presence of coliforms which is associated with diarrheal infections due to fecal contamination. We examined the water samples by Membrane filter method in which we use 0.45µm filter paper which was soaked in peptone water and incubated at 37 oC for 24 hours, this peptone water was further streaked on MacConkey and EMB media. Additional identification of microorganisms was done by microscopy and biochemical tests. Observed results revealed that the water is fecally contaminated and the microorganisms isolated were as with described percentages E.coli 12%, Klebsiella 40%, Proteus 44%, Pseudomonas 20%, Shigella 8%, Salmonella 4% and Enterobacter 4% who are the members of coliforms. This study helps in the identification of coliform from different sources which lead to a cause of diarrheal infectio