Additional file 1: of Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis

Figure S1. Equine keratinocytes (EK-100) were cultured in various culture conditions. Representative phase contrast images of primary equine keratinocytes (EK-100) cultured in (a) co-culture with irradiated fibroblasts+ 10 uM Y-27632, (b) F + 10 uM Y-27632 (c,d) CNT with or without 10 uM Y-27632 and (e)KSFM+ 10 uM. All images were taken on and day7 following initial culture without passage (×10 magnification. Size bars = 400 μm). Top right images show enlarged magnification (×40 magnification, size bars = 100 μm). Figure S2. Fluorescence-activated cell sorting (FACS) analysis of human keratinocytes (HFK) and mouse fibroblasts (j2) using pan-cytokeratin antibody. HFK cells were incubated without (a) pan-CK antibody or (b) with pan-CK antibody, (c) J2 fibroblasts without pan-CK antibody, or (d) with pan-CK antibody. Figure S3. Validation of antibodies for equine tissues. Specificity and reactivity of CK-14 was tested by using diluted concentration of CK14 1:600, 1:5000, and no antibody respectively in (a) breast cancer tissue and (b) equine skin tissue. All images (×40 magnification, scale bar = 100 μm) are representative of three experimental repeats. (PPTX 5023 kb