Safety Measures for Operating Team and Operation Theater During the Current Scenario of COVID-19

The COVID-19 has become a major threat to Pakistan and worldwide, and has become a significant issue for global health, economy and societies. This rapid spread was occurred from Wuhan, China to most of the part of the world. To elaborate the concept and recommendations regarding the safety precautions in operation theater (O.T) and inside associated team during the current scenario of COVID-19. Many research and review articles were studied to collect information about Covid-19 and strategies published in various journals using the search engine, PubMed and Medline. The COVID-19 has significantly changed all aspects of daily life around the world since very start of this year 2020. SARS-CoV-2 (COVID-19), a novel corona virus, has been infected many healthcare workers. In this perspective, hospitals need a strategy to manage their resources, staff and supplies so that patients receive optimal treatment. A decision tree algorithm was developed that defined the recommendations for safety measures in operation theater and operating procedures, these include identifying and developing an isolation room, administrative measures such as transformations in working flow and procedures, introducing personal protective equipment for the employees and formulating anesthetic clinical guidelines. These control actions are essential to enhance the excellence of care provided to COVID-19 patients and to minimize the risk of spread to other patients or staff. The operating room is a dynamic environment with numerous staff like anesthesiologists, physicians, nurses, O.T attendants and technicians; however, we agree that the containment steps are important in order to improve the standard of treatment provided to COVID-19 patients and to minimize the chance of viral spread to patients other than COVID-19 and hospital staff

The Impact of Bypass Fat on Milk Contents And Yield in Indigenous Buffalo (Bubalus Bubalis) of Sindh, Pakistan

Introduction: Buffalo and cow milk have a great affinity for strong bones, healthy teeth and in various heart diseases. The study was carried out on Kundi buffalo to assess the outcomes of bypass fat on dry matter, milk output, milk contents, body mass gain, nutrient digestibility, and production costs. Methodology: 12 buffaloes of early third and fourth lactation were selected, divided into two groups (six each) for ninety days experiment. In the test animals, additional 300 mg bypass fat was given with balance ration containing 16% of crude proteins, while in control group only balance routine ration was provided. The data was statistically evaluated using a one-way analysis of variance in a completely randomized design (CRD), and differences were assessed using a t-test. Results: The results indicated that mean values in group-A regarding the dry matter intake (DMI) was significantly elevated (P<0.05) (14.29±1.42 kg) as compared to control group (13.65 ± 2.174 kg). At the end of experiment the mean of daily milk production (7.73±1.115 liters/day) was also reported higher (P<0.05) in group-A. The milk yield of Group-A was raised up to 15.39% than the control group. Though, the buffaloes under bypass fat treatment were not found significantly higher in weight gain, but the milk contents in context of solids not fat, fat and total solids presented as P<0.05 between the treatments. Conclusion: Therefore, it is concluded from the conducted study that, bypass fat can be employed to produce milk both quantitatively and qualitatively at a reasonable cost

Genetic Diversity Relationship in Azakheli Buffalo Inferred from mtDNA and MC1R Sequences Comparison

Azakheli is relatively smaller riverine breed with a very peculiar characteristics kept under unique traditional husbandry practices in comparison with rest of the Pakistani buffalo breeds; however, milk production is comparable. The present study was conducted to evaluate the genetic diversity of Azakheli breed. A total of sixty-six blood samples were collected for the amplification of mtDNA D-loop region and MC1R gene sequencing analysis. Median-joining network analysis of 191 mtDNA D-loop sequences of Azakheli and eight Indian riverine buffalo breeds clustered into three clades. Ancient Azakheli Region 1 clade was the oldest with the highest mutation steps and was present close to the root of UPGMA phylogenetic tree. There was 5 mutated lines distance between Pakistan buffalo and Indian riverine buffaloes. The populations of neighboring countries did not share any haplotypes with Azakheli buffalo of Pakistan. Possibly, residing for so long in the cold atmosphere and high elevation regions caused the mutation in mtDNA D-loop, though these conditions did not affect the overall performance of Azakheli as milch buffalo breed of Pakistan. MC1R analyses showed high mutations in Azakheli of Albino phenotype and all the black phenotype individuals of Azakheli buffalo share haplotypes with dominant Chinese and Indian black phenotypes buffaloes in MC1R median-joining network, indicating the reason of black coat color is due to MC1R gene. The haplotype diversity and nucleotide diversity was (H. 0.923, Pi: 0.00895) in Azakheli. Current results illustrated Asian ancestry for Azakheli buffalo, and mtDNA and MC1R analyses provided further evidence. Additional genetic analyses and archeological studies may provide further insight into the domestication period and history of Azakheli buffalo breed. The further studies are required on different coat colors with different genes on Azakheli buffalo to understand the phenotype variation