Standardisasi dan Uji Aktivitas Antioksidan Ekstrak Bahan Alam Propolis untuk Terapi Infeksi SARS-CoV2

Natural ingredients with immunomodulatory, anti-inflammatory, and antioxidant activities can be used as supportive therapy for SARS-CoV2 infection. The aims were standardized the quality and safety of propolis and determined the antioxidant activity of propolis. There are two types of propolis powder samples: microencapsulated propolis powder containing wax (SMPW) and non-waxed (SMP). Standardization includes specific and non-specific parameters. Antioxidant activity test using FRAP and ABTS methods. The total flavonoids of SMPW and SMP were 0.31% and 1.59%. The total phenolic of SMPW and SMP were 83.9082 and 98.0821 mg GAE/g. The losses on drying of SMPW and SMP were 3.65% and 3.88%. The water content of SMPW and SMP were 5.12% and 5.11%. The total ash content of SMPW and SMP were 0.80% and 0.65%. Heavy metal propolis powder negative. ALT and AKK values < 10 CFU/g, microbiology contamination was negative. Antioxidant activity of SMPW with IC50 ABTS 2.4177 ppm and EC50 FRAP 26.41 g/mL. Meanwhile, the antioxidant activity of SMP with IC50 ABTS 2.213 ppm and EC50 FRAP 32.10 g/mL. It was concluded that the extract of propolis met the requirements of the standard for extracts of natural ingredients and had strong antioxidant activity. Propolis powder can be developed for supportive therapy for SARS-CoV2 infection.Bahan alam dengan aktivitas imunomodulator, antiinflamasi, dan antioksidan dapat digunakan sebagai terapi suportif infeksi SARS-CoV2. Tujuan penelitian standardisasi mutu dan keamanan propolis sebagai bahan baku obat terapi suportif Covid-19 serta uji aktivitas propolis sebagai antioksidan. Sampel serbuk propolis terdiri dari dua jenis: serbuk mikroenkapsulasi propolis mengandung wax (SMPW) dan non wax (SMP). Standardisasi meliputi parameter spesifik dan non spesifik. Uji aktivitas antioksidan menggunakan metode FRAP dan ABTS. Kadar total flavonoid SMPW dan SMP sebesar 0,31% dan 1,59%. Kadar total fenolik SMPW dan SMP sebesar 83,9082 dan 98,0821 mg GAE/g. Susut pengeringan SMPW dan SMP sebesar 3,65% dan 3,88%. Kadar air SMPW dan SMP sebesar 5,12% dan 5,11%. Kadar abu total SMPW dan SMP sebesar 0,80% dan 0,65%. Logam berat serbuk propolis negatif. Nilai ALT dan AKK < 10 CFU/g, cemaran E. Coli, S. aureus, Salmonella spp dan P. aeruginusa negatif. Aktivitas antioksidan SMPW dengan IC50 ABTS 2,4177 bpj dan EC50 FRAP 26,41 μg/mL. Sedangkan, aktivitas antioksidan SMP dengan IC50 ABTS 2,213 bpj dan EC50 FRAP 32,10 μg/mL. Disimpulkan ekstrak bahan alam propolis memenuhi persyaratan standard ekstrak bahan alam dan memiliki aktivitas antioksidan yang kuat. Serbuk EBA propolis dapat dikembangkan menjadi bahan baku untuk OHT terapi suportif infeksi SARS-CoV2