A large area, high counting rate micromegas-based neutron detector for BNCT

Beam monitoring and evaluation are very important to boron neutron capture therapy (BNCT), and a variety of detectors have been developed for these applications. However, most of the detectors used in BNCT only have a small detection area, leading to the inconvenience of the full-scale 2-D measurement of the beam. Based on micromegas technology, we designed a neutron detector with large detection area and high counting rate. This detector has a detection area of 288 mm multiples 288 mm and can measure thermal, epithermal, and fast neutrons with different detector settings. The BNCT experiments demonstrated that this detector has a very good 2-D imaging performance for the thermal, epithermal, fast neutron and gamma components, a highest counting rate of 94 kHz/channel, and a good linearity response to the beam power. Additionally, the flux fraction of each component can be calculated based on the measurement results. The Am-Be neutron source experiment indicates that this detector has a spatial resolution of approximately 1.4 mm, meeting the requirements of applications in BNCT. It is evident that this micromegas-based neutron detector with a large area and high counting rate capability has great development prospects in BNCT beam monitoring and evaluation applications

Metabolomics-based discovery of XHP as a CYP3A4 inhibitor against pancreatic cancer

Background: Xihuang Wan (XHW), a purgative and detoxifying agent, is commonly utilized in modern medicine as a treatment and adjuvant therapy for various malignancies, including breast cancer, liver cancer, and lung cancer. A clinical study demonstrated the potential usefulness of the combination of XHW and gemcitabine as a therapy for pancreatic cancer (PC), indicating that XHW’s broad-spectrum antitumor herbal combination could be beneficial in the treatment of PC. However, the precise therapeutic efficacy of XHW in treating pancreatic cancer remains uncertain.Aim: This study assessed the biological activity of XHW by optimizing the therapeutic concentration of XHW (Xihuang pills, XHP). We performed cell culture and developed an animal test model to determine whether XHP can inhibit pancreatic cancer (PC). We also applied the well-known widely targeted metabolomics analysis and conducted specific experiments to assess the feasibility of our method in PC therapy.Materials and Methods: We used UPLC/Q-TOF-MS to test XHP values to set up therapeutic concentrations for the in vivo test model. SW1990 pancreatic cancer cells were cultured to check the effect the anti-cancer effects of XHP by general in vitro cell analyses including CCK-8, Hoechst 33258, and flow cytometry. To develop the animal model, a solid tumor was subcutaneously formed on a mouse model of PC and assessed by immunohistochemistry and TUNEL apoptosis assay. We also applied the widely targeted metabolomics method following Western blot and RT-PCR to evaluate multiple metabolites to check the therapeutic effect of XHP in our cancer test model.Results: Quantified analysis from UPLC/Q-TOF-MS showed the presence of the following components of XHP: 11-carbonyl-β-acetyl-boswellic acid (AKBA), 11-carbonyl-β-boswellic acid (KBA), 4-methylene-2,8,8-trimethyl-2-vinyl-bicyclo [5.2.0]nonane, and (1S-endo)-2-methyl-3-methylene-2-(4-methyl-3-3-pentenyl)-bicyclo [2.2.1heptane]. The results of the cell culture experiments demonstrated that XHP suppressed the growth of SW1990 PC cells by enhancing apoptosis. The results of the animal model tests also indicated the suppression effect of XHP on tumor growth. Furthermore, the result of the widely targeted metabolomics analysis showed that the steroid hormone biosynthesis metabolic pathway was a critical factor in the anti-PC effect of XHP in the animal model. Moreover, Western blot and RT-PCR analyses revealed XHP downregulated CYP3A4 expression as an applicable targeted therapeutic approach.Conclusion: The results of this study demonstrated the potential of XHP in therapeutic applications in PC. Moreover, the widely targeted metabolomics method revealed CYP3A4 is a potential therapeutic target of XHP in PC control. These findings provide a high level of confidence that XHP significantly acts as a CYP3A4 inhibitor in anti-cancer therapeutic applications

Role of Glutathione-Ascorbate Cycle and Photosynthetic Electronic Transfer in Alternative Oxidase-Manipulated Waterlogging Tolerance in Watermelon Seedlings

Alternative oxidase (AOX) has been documented to mitigate the oxidative stress caused by abiotic stresses. However, it remains unknown how AOX regulates the antioxidant system and photosynthesis under waterlogging. To address this issue, we used two watermelon (Citrullus lanatus L.) cultivars (waterlogging tolerant cultivar ‘YL’ and sensitive cultivar ‘Zaojia8424’) as materials and the AOX inhibitor salicylhydroxamic acid (SHAM) to investigate the effects of AOX on photosynthesis and reactive oxygen species metabolism under waterlogging. We found that waterlogging decreased leaf photosynthesis and quantum yield of photosynthesis in watermelon, and the waterlogging tolerant cultivar ‘YL’ showed higher expression level of ClaAOX than the sensitive cultivar ‘Zaojia8424’. Net photosynthesis rate was higher in ‘YL’ than ‘Zaojia8424’. Moreover, waterlogging induced photoinhibition in ‘Zaojia8424’ but not in ‘YL’. Meanwhile, waterlogging promoted the accumulation of superoxide and peroxide hydrogen, and triggered oxidative damage. ‘YL’ suffered from less severe oxidative damage due to increased contents of ascorbate, a higher ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG), a higher activity of ascorbate peroxidase (APX) and catalase (CAT), and enhanced levels of CAT and APX expression, relative to ‘Zaojia8424’. However, the alleviation of photosynthesis and oxidative damage, increased content of ascorbate and higher GSH/GSSG ratio were abolished by SHAM. Our results suggested that photosynthetic electronic transfer and glutathione-ascorbate cycle are involved in waterlogging tolerance mediated by the AOX pathway in watermelon

The Effects of Tai Chi Intervention on Healthy Elderly by Means of Neuroimaging and EEG: A Systematic Review

Aging is a process associated with a decline in cognitive and motor functions, which can be attributed to neurological changes in the brain. Tai Chi, a multimodal mind-body exercise, can be practiced by people across all ages. Previous research identified effects of Tai Chi practice on delaying cognitive and motor degeneration. Benefits in behavioral performance included improved fine and gross motor skills, postural control, muscle strength, and so forth. Neural plasticity remained in the aging brain implies that Tai Chi-associated benefits may not be limited to the behavioral level. Instead, neurological changes in the human brain play a significant role in corresponding to the behavioral improvement. However, previous studies mainly focused on the effects of behavioral performance, leaving neurological changes largely unknown. This systematic review summarized extant studies that used brain imaging techniques and EEG to examine the effects of Tai Chi on older adults. Eleven articles were eligible for the final review. Three neuroimaging techniques including fMRI (N = 6), EEG (N = 4), and MRI (N = 1), were employed for different study interests. Significant changes were reported on subjects' cortical thickness, functional connectivity and homogeneity of the brain, and executive network neural function after Tai Chi intervention. The findings suggested that Tai Chi intervention give rise to beneficial neurological changes in the human brain. Future research should develop valid and convincing study design by applying neuroimaging techniques to detect effects of Tai Chi intervention on the central nervous system of older adults. By integrating neuroimaging techniques into randomized controlled trials involved with Tai Chi intervention, researchers can extend the current research focus from behavioral domain to neurological level

Inkjet-printed O2 gas sensors in intelligent packaging

This study was supported by the Spanish Ministerio de Economia y Competitividad (Projects PID2019-103938RB-I00 and CTQ2017-86125-P) and Junta de Andalucia (Projects B-FQM-243-UGR18 and P18-RT-2961). The projects were partially supported by European Regional Development Funds (ERDF).An inkjet printed membrane is presented as a colorimetric sensor for oxygen for use in smart packaging, in order to quickly inform the consumer about possible degradation reactions in modified atmosphere products (MAP). The colorimetric sensor is based on the redox dye, toluidine blue (TB), a sacrificial electron donor, glycerol, and, hydroxypropyl methylcellulose, as the hydrophilic polymeric matrix. The UVC-wavelength activated TB is photoreduced by SnO2 nanoparticle ink. This colorimetric oxygen indicator stays colourless upon exposure in nitrogen atmosphere to 7 min UVC light (6 W center dot cm(-2)). The photoreduced TB to leuco TB recovers its original colour upon exposure to oxygen for 55 min under ambient conditions (similar to 21 degrees C, similar to 55%RH, 21% O-2). The characteristics of the sensor have been evaluated, including its functionality through the colorimetric response to different oxygen concentrations as well as the influence of experimental variables such as humidity and temperature using a digital camera as the detector. The results obtained show that: (1) the colorimetric sensor remains stable in the absence of oxygen; (2) relative humidity greater than 60% significantly affects the reoxidation process; and (3) the temperature has a significant influence on the colour recovery, although the stability increases considerably when the sensor is kept refrigerated at 4 degrees C. A real application to packaged ham was performed, demonstrating that the printed colorimetric sensor is stable for at least 48 hours once activated and when the container deteriorates upon the entrance of oxygen, the sensor returns to its original blue colour, demonstrating its utility as a UVC-activated colorimetric oxygen sensor.Spanish Ministerio de Economia y Competitividad PID2019-103938RB-I00 CTQ2017-86125-PJunta de Andalucia B-FQM-243-UGR18 P18-RT-2961European Commissio

Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice

To identify the chemical components responsible for the anti-hyperglycemic effect of Cyclocarya paliurus (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of C. paliurus leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H&E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of C. paliurus leaves, and the clinical application of C. paliurus need to be refined