Idiopathic pulmonary fibrosis: a disease with similarities and links to cancer biology

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Phytosanitary rules for grapevine (Vitis vinifera L.) propagation material introduction into EU for germplasm conservation and scientific purposes

Annex

Evolution of smart strategies and machines used for conservative management of herbaceous and horticultural crops in the mediterranean basin: a review

Preserving soil quality and increasing soil water availability is an important challenge to ensure food production for a growing global population. As demonstrated by several studies, conservative crop management, combined with soil cover and crop diversification, can significantly reduce soil and water losses. The aim of this review is to evaluate the spread of smart solutions to perform conservation agriculture (CA) well in the Mediterranean Basin, taking into account the evolution of the strategies and the machines used to perform conservative tillage systems in different contexts in the last 30 years, as well as the effects induced by their adoption on the productivity of herbaceous and horticultural crops, weed control and economic feasibility, in comparison with those obtained utilizing conventional techniques based on soil inversion

Output-only identification of rigid body motions of floating structures: a case study

In order to identify rigid body motions of floating offshore structures, output-only techniques are very useful for developing low-cost intermediate-scale experimental activities directly into the sea, instead of wave tanks. A crucial parameter, however, is the length of the response records used as input for the identification process, since short records may result in significant loss of accuracy, while long ones may be incompatible with the assumption of stationarity of the sea state. This work presents a sensitivity study conducted on a numerical model of a spar structure, identified by means of Enhanced Frequency Domain Decomposition method. An overview on the efficiency of the method is given for various lengths of response record, along with practical indications on the minimum values acceptable

On intermediate-scale open-sea experiments on floating offshore structures: Feasibility and application on a spar support for offshore wind turbines

Experimental investigation of floating structures represents the most direct way for achieving their dynamic identification and it is particularly valuable for relatively new concepts, such as floating supports for offshore wind turbines, in order to fully understand their dynamic behaviour. Traditional experimental campaigns on floating structures are carried out at small scale, in indoor laboratories, equipped with wave and wind generation facilities. This article presents the results of an open-sea experimental activity on a 1:30 scale model of the OC3-Hywind spar, in parked rotor conditions, carried out at the Natural Ocean Engineering Laboratory (NOEL) of Reggio Calabria (Italy). The aim of the experiment is two-fold. Firstly, it aims to assess the feasibility of low-cost, intermediate-scale, open-sea activities on offshore structures, which are proposed to substitute or complement the traditional indoor activities in ocean basins. Secondly, it provides useful experimental data on damping properties of spar support structures for offshore wind turbines, with respect to heave, roll and pitch degrees of freedom. It is proven that the proposed approach may overcome some limitations of traditional small-scale activities, namely high costs and small scale, and allows to enhance the fidelity of the experimental data currently available in literature for spar floating supports for offshore wind turbines

Multiple sclerosis treatment and melanoma development

Therapy of multiple sclerosis (MS) with disease-modifying agents such as natalizumab or fingolimod has been associated with the development of cutaneous melanoma. Here we briefly revise literature data and report of a case of a 48-year old woman who developed a melanoma and several atypical naevi after sub sequential treatment with natalizumab (1 year) and fingolimod (7 years). By immunohistochemistry we observed the presence of T cells and leukocyte infiltration as well as of vascular endothelial growth factor (VEGF)-A expression in the patient melanoma biopsy. Then, we analyzed proliferation, migration and VEGF-A expression in three melanoma cell lines and found out that both natalizumab and fingolimod inhibited tumor cell proliferation but promoted or blocked cell migration depending on the cell line examined. VEGF-A secretion was augmented in one melanoma cell line only after fingolimod treatment. In conclusion, our in vitro data do not support the hypothesis of a direct action of natalizumab or fingolimod on melanoma progression but acting on the tumor microenvironment these treatments could indirectly favor melanoma evolution

PFN1 and integrin-β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells

Ex vivo limbal stem cell transplantation is the main therapeutic approach to address a complete and functional re-epithelialization in corneal blindness, the second most common eye disorder. Although important key points were defined, the molecular mechanisms involved in the epithelial phenotype determination are unclear. Our previous studies have demonstrated the pluripotency and immune-modulatory of fibroblast limbal stem cells (f-LSCs), isolated from the corneal limbus. We defined a proteomic profile especially enriched in wound healing and cytoskeleton-remodelling proteins, including Profilin-1 (PFN1). In this study we postulate that pfn-1 knock down promotes epithelial lineage by inhibiting the integrin-β1(CD29)/mTOR pathway and subsequent NANOG down-expression. We showed that it is possible modulate pfn1 expression levels by treating f-LSCs with Resveratrol (RSV), a natural compound: pfn1 decline is accompanied with up-regulation of the specific differentiation epithelial genes pax6 (paired-box 6), sox17 (sex determining region Y-box 17) and ΔNp63-α (p63 splice variant), consistent with drop-down of the principle stem gene levels. These results contribute to understand the molecular biology of corneal epithelium development and suggest that pfn1 is a potential molecular target for the treatment of corneal blindness based on epithelial cell dysfunction

Expression and Function of Neurotrophins and Their Receptors in Cultured Human Keratinocytes

Whereas nerve growth factor has been extensively studied in human keratinocytes, little is known on the role of other members of the neurotrophin family. We investigated the expression and function of neurotrophins and neurotrophin receptors in cultured human keratinocytes. We demonstrated by reverse transcription–polymerase chain reaction that keratinocytes synthesize neurotrophin-3, brain-derived neurotrophic factor, and neurotrophin-4/5. These cells also express tyrosinase kinase A and C, the nerve growth factor and neuro-trophin-3 high-affinity receptors, respectively. On the other hand, only the truncated extracellular isoform of tyrosinase kinase B, the high-affinity brain-derived neurotrophic factor and neurotrophin-4/5 receptor, is detected in keratinocytes. Moreover, neurotrophin-3, brain-derived neurotrophic factor, and neurotrophin-4/5 proteins are secreted by human keratinocytes at low levels. Keratinocyte stem cells synthesize the highest amounts of nerve growth factor, while they secrete higher levels of nerve growth factor as compared with transit amplifying cells. Neurotrophin-3 stimulates keratinocyte proliferation, where brain-derived neurotrophic factor or neurotrophin-4/5 does not exert any effect on keratinocyte proliferation. Addition of neurotrophin-3 slightly upregulates the secretion of nerve growth factor, whereas nerve growth factor strongly augments neurotrophin-3 release. Ultraviolet B irradiation downregulates nerve growth factor, whereas it augments neurotrophin-3 and neurotrophin-4/5 protein levels. Ultraviolet A irradiation increases the level of neurotrophin-3, whereas it does not exert any effect on the other neurotrophins. Finally, neurotrophins other than nerve growth factor fail to protect human keratinocytes from ultraviolet B-induced apoptosis. This work delineates a functional neurotrophin network, which may contribute to epidermal homeostasis

Bioaccessibility of provitamin A carotenoids in orange-fleshed pumpkins using the coupled In vitro digestion/ Caco-2 cell model.

The aim of this study was to evaluate the effects of cooking styles on the bioacessibility of BC in pumpkin