Fascin expression in pleomorphic adenoma and mucoepidermoid carcinoma

Background: Salivary gland tumors constitute an important part of oral and maxillofacial pathology. Pleomorphic adenoma (PA) and mucoepidermoid carcinoma (MEC) are the most common benign and malignant salivary gland tumors. Fascin is an actin-bundling protein that increases the motility of normal and transformed epithelial cells. The aims of the study were to determine the expression of fascin in these tumors and to determine its role in their progression. Materials and Methods: A total of 40 formalin-fixed, paraffin-embedded tissue blocks of PA, and 20 blocks of MEC were included in this study. Diagnostic confirmation was performed through examination of hematoxylin and eosin sections. Both tumors were immunohistochemically analyzed for the presence of fascin using Avidin-Biotin complex method and evaluated via light microscope by 2 independent observers. Statistical analysis was performed using Kruskal-Wallis and Chi-square tests with significant level of P < 0.05. Results: In both the tumors, the percentage of stained cells was significantly correlated with intensity of staining (P = 0.01 in PA and P = 0.00 in MEC). In PA, statistical analysis showed a significant direct correlation between percentage of stained cells and recurrence (P = 0.00). There was no significant correlation between intensity and percentage of staining with clinicopathologic factors in MEC. Conclusion: Fascin might be a useful marker for recurrence of PAs and patients with high fascin expression in primary PA should be followed up periodically to detect potential recurrence as soon as possible

Assessment of biochemical factors in blood serum of patients with oral squamous cell carcinoma

Background: Recently, the role of biochemical factors in the etiology of oral squamous cell carcinoma (OSCC) has attracted some attention. Serum levels of biochemical factors may change in cancer patients. This study aimed to assess the serum level of folate, Vitamin B12, homocysteine, iron, copper, and selenium in patients with OSCC. Materials and Methods: This descriptive analytical study was conducted on 30 primary OSCC patients (15 males and 15 females) presenting to Imam Khomeini Cancer Institute, who had not yet undergone treatment. Blood samples were taken and serum levels of folate, Vitamin B12, homocysteine, iron, copper, and selenium were measured. Serum levels of micronutrients in patients with different tumor sizes were analyzed by one-way ANOVA. Serum levels of micronutrients were compared among groups with and without metastasis and lymph node involvement using Student's t-test (P < 0.05). Results: Serum levels of B12, folic acid, homocysteine, copper, iron, and selenium were 232.5 ± 102.68, 8.66 ± 4.06, 18.87 ± 8.81, 96.0 ± 22.64, 55.27 ± 40.58, and 92.47 ± 18.83 ng/mL, respectively. Relatively similar values were measured in patients with different tumor sizes with and without lymph node involvement and presence or absence distant metastasis. However, the serum level of folic acid in OSCC patients without lymph node involvement was significantly higher than that in OSCC patients with lymph node involvement (P < 0.05). Conclusion: Despite some variations, serum levels of micronutrients in OSCC patients were within the normal limits. Considering the variations in serum level of copper in OSCC patients, it may be used as a diagnostic marker. However, further studies are warranted in this respect