The Effect of Orally Administered Lactobacillus rhamnosus as “Systemic Probiotics” on C-Reactive Protein Levels and Wound Healing of Oral Mucosa in Rabbits

Aims: This study investigates the beneficial role of Lactobacillus rhamnosus in oral wound healing. Materials and methods: Twenty-five rabbits were divided into three main groups; The First was the Negative Control group(group I) with five rabbits, these had neither surgery nor treatment. The second was the Positive control group(group II) with ten rabbits, these had an incisional cut of 1cm in their buccal mucosa but none had received any treatment. The rabbits in this group were subdivided into two subgroups (a and b) with five animals each according to the time of euthanasia, as explained below. The third was the treatment group (group III) with ten rabbits. These all had the same oral incision and received probiotic drops (VITRON® Farma, Turkey); an already prepared solution containing Lactobacillus rhamnosus, 5 oral drops (0.25 ml)/kg to be swallowed once daily starting from the first day of study till the day of euthanizing. Animals in this group were also subdivided equally into a and b subgroups according to the time of euthanasia. The rabbits from group I and subgroups IIa and IIIa were euthanized on the third-day post-surgery. All other rabbits from subgroups IIb and IIIb were euthanized on the seventh-day post-surgery. Serum samples were collected and mucosal tissue at wound sites was excised for histological analysis. Results: The serum levels of C-reactive protein (CRP) in the third-day groups of the study indicated a highly significant difference (P ≤0.001), as well as between the groups on the seventh day which also showed a noticeable decrease in the concentration of these cytokines. A microscopic inspection in the Positive Control group after three days revealed a wound with inflammatory exudate, fibrin deposition, and destruction of the epithelium layer of mucosa with slight re-epithelialization. The seventh-day tissue of the positive control group showed highly inflammatory cell infiltration, fibrin deposition, reepithelialization, and minimal granulation tissue formation. In contrast, the treatment group's mucosal wounds on three postsurgical days showed a wound site with little inflammatory exudate, early signs of reepithelialization, and well-formed granulation tissue, while on day seven showed a wound site with no inflammatory exudate, well-developed re-epithelialization, well granulation tissue formation and signs of angiogenesis. Conclusion: Systemic probiotic Lactobacillus rhamnosus enhanced the wound healing of oral mucosa in rabbits

Effects of Dpyridamole on Histopathology of Salivary Glands in Rabbits

Aims: Dipyridamole is a well-known coronary vasodilator. It is an adenosine reuptake inhibitor leading to increased blood levels of adenosine. So, this study aims to investigate the effects of systemic administration of dipyridamole on the salivary gland tissues in the rabbit model. Materials and methods: Ten male rabbits with a body weight of 1.50 ± 0.25kg were involved. The control group (5 animals) received no treatment, while the treatment group (5 animals) was treated with dipyridamole by gavage tube at an oral dose of 8 mg/kg once daily for 30 days. Then all animals were sacrificed and tissue sample sections from the salivary glands were subjected to a hematoxylin-eosin stain and evaluated for histopathological examination. Results: Histopathological slides of parotid and submandibular glands in the treatment group showed abnormal changes in the structure of acinar cells and the cells lining the striated ducts. Sections of parotid displayed necrosis of the cells of serous acini and the cells lining the striated ducts, with the presence of hemorrhage around these ducts. Conclusions: Increased levels of adenosine in the body microenvironment induced by systemic administration of the dipyridamole can cause salivary gland tissue disturbance

Protective effects of vitamin C against oxidative stress induced by diethylene glycol in liver and kidney

This study aims to investigate the vitamin c protective influence against oxidative stress, liver and kidney destructions made by diethylene glycol in rabbit model. Eighteen healthy male rabbits were grouped into 3 classes. Control group: without any treatment. DEG group were treated with 40 mg /kg /day of DEG for 14 days while DEG with vitamin C group with 40 mg /kg /day of DEG with 10mg /kg/day of vitamin C for 14 days.  All animals were euthanized at day 14 of the study. Venous blood samples (5ml) were collected from rabbits via jugular vein and serum samples were isolated and kept at -20 ˚C till time of analysis. Serum analysis was done by colorimetric Assay kit for total antioxidant capacity (TAC). Tissue samples from liver and kidneys were isolated, pigmented by hematoxylin and eosin (H & E) pigments and investigated under a light microscope for histopathological changes. Results: The TAC in the treatment group was significantly reduced at 14th day of study when compared to the other groups. While non- significant differences were found between control and treatment with vitamin C groups