Pch2 Links Chromosome Axis Remodeling at Future Crossover Sites and Crossover Distribution during Yeast Meiosis

Segregation of homologous chromosomes during meiosis I depends on appropriately positioned crossovers/chiasmata. Crossover assurance ensures at least one crossover per homolog pair, while interference reduces double crossovers. Here, we have investigated the interplay between chromosome axis morphogenesis and non-random crossover placement. We demonstrate that chromosome axes are structurally modified at future crossover sites as indicated by correspondence between crossover designation marker Zip3 and domains enriched for axis ensemble Hop1/Red1. This association is first detected at the zygotene stage, persists until double Holliday junction resolution, and is controlled by the conserved AAA+ ATPase Pch2. Pch2 further mediates crossover interference, although it is dispensable for crossover formation at normal levels. Thus, interference appears to be superimposed on underlying mechanisms of crossover formation. When recombination-initiating DSBs are reduced, Pch2 is also required for viable spore formation, consistent with further functions in chiasma formation. pch2Δ mutant defects in crossover interference and spore viability at reduced DSB levels are oppositely modulated by temperature, suggesting contributions of two separable pathways to crossover control. Roles of Pch2 in controlling both chromosome axis morphogenesis and crossover placement suggest linkage between these processes. Pch2 is proposed to reorganize chromosome axes into a tiling array of long-range crossover control modules, resulting in chiasma formation at minimum levels and with maximum spacing

Interferometric measurement of ionization in a grassfire

Grassfire plumes are weakly ionized gas. The ionization in the fire plume is due to thermal and chemi-ionization of incumbent species, which may include graphitic carbon, alkalis and thermally excited radicals, e.g., methyl. The presence of alkalis (e.g., potassium and sodium) in the fires makes thermal ionization a predominant electron producing mechanism in the combustion zone. Alkalis have low dissociation and ionization potentials and therefore require little energy to thermally decompose and give electrons. Assuming a Maxwellian velocity distribution of flame particles and electron-neutral collision frequency much higher than plasma frequency, the propagation of radio waves through a grassfire is predicted to have attenuation and phase shift. Radio wave propagation measurements were performed in a moderate intensity (554 kW m^−1) controlled grassfire at 30- and 151-MHz frequencies on a 44 m path using a radio wave interferometer. The maximum temperature measured in the controlled burn was 1071 K and the observed fire depth was 0.9 m. The radio wave interferometer measured attenuation coefficients of 0.033 and 0.054 dB m^−1 for 30- and 151-MHz, respectively. At collision frequency of 1.0 × 10^11 s^−1, maximum electron density was determined to be 5.061 × 10^15 m^−3