4 research outputs found
Molecular characterization and SNP development for the porcine IL6 and IL10 genes
Different cytokines are secreted in response to specific microbial molecules referred to as pathogen associated molecular patterns (PAMPs). Interleukin 6 (IL6) and interleukin 10 (IL10), both secreted by macrophages and lymphocytes, play a central role in the immunological response. In this work we obtained the genomic structure and complete DNA sequence of the porcine IL6 and IL10 genes and identified polymorphisms in the genomic sequences of these genes on a panel of ten different pig breeds. Comparative intra- and interbreed sequence analysis revealed a total of eight polymorphisms in the porcine IL6 gene and 21 in the porcine IL10 gene, which include single nucleotide polymorphisms (SNPs) and insertion deletion polymorphisms (indels). Additionally, the chromosomal localization of the IL10 gene was determined by FISH and RH mapping
Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance to Actinobacillus pleuropneumoniae infection
Biomarkers facilitating both pathogen-independent diagnosis of
respiratory health and breeding
selection of pigs with increased resistance to respiratory tract infections
would be of considerable interest to
the pig industry. Following this concept we performed a comparative
glycoproteome analysis of
bronchoalveolar lavage fluid (BALF) from healthy pigs and pigs 4 days (acute)
and 20 days (chronic) after
an experimental infection with Actinobacillus pleuropneumoniae. In order to
identify possible differences in
BALF glycoprotein patterns we investigated pigs of three different breeding
lines (German Landrace,
Piétrain, Hampshire). In total, 12 glycosylated proteins (alpha-1-acid
glycoprotein, fetuin A, properdin,
haptoglobin precursor, haptoglobin, hemoglobin, hyaluronidase,
inter-alpha-trypsin inhibitor family heavy
chain-related protein, alpha-1-antichymotrypsin 3, pulmonary
surfactant-associated protein D (SP-D),
transferrin, and alpha-1B-glycoprotein) were identified as being
differentially expressed depending on the
health status of the animal. Fetuin A levels were consistently low in
chronically infected pigs thereby being
a potential marker for chronic infection. Hyaluronidase levels were
consistently high in all pigs after
experimental infection independent on isolation of the pathogen thereby
being a potential marker for
previous pathogen contact and latent infection. High levels of fetuin A as
well as low levels of haptoglobin
and pulmonary SP-D correlated with the absence of lung lesions in pigs of
the Hampshire breeding line,
implying a potential application as selection markers for breeding programmes