Infertilité masculine chez les patients normospermiques : analyse protéomique des spermes normaux non fécondants en fécondation in vitro classique

PARIS-BIUP (751062107) / SudocSudocFranceF

Devenir des grossesses uniques après transfert au stade blastocyste : comparaison avec les transferts précoces

Objectifs Le transfert frais de blastocystes a montré son intérêt dans la prise en charge des couples en FIV. Or peu de données ont été publiées sur le déroulement de la grossesse et l’état des enfants nés. Ce travail compare le déroulement de la grossesse, l’accouchement et l’état des enfants à la naissance après transfert au stade blastocyste avec le transfert embryonnaire précoce (à j2). Patientes et méthodes Il s’agit d’une étude rétrospective menée au sein du service d’AMP du CHRU de Tours de janvier 2002 à juin 2009. Il a été comparé le devenir de 1547 grossesses uniques chez des femmes primipares et de même âge issues d’un transfert au stade blastocyste (n = 588) ou au stade précoce à j2 (n = 959). Les données proviennent de l’analyse des fiches grossesses FIVNAT retournées volontairement par les couples. Résultats Avec un retour de fiches grossesses FIVNAT de 97,6 %, nous avons observé que le déroulement de la grossesse, le sex-ratio, le poids de naissance et l’état de l’enfant à la naissance n’étaient pas affectés par le stade embryonnaire transféré dans le cadre de grossesses uniques. Discussion et conclusion En ce qui concerne le déroulement de la grossesse, l’accouchement et l’état des enfants nés après transfert au stade blastocyste, les données de la littérature sont peu abondantes, souvent controversées et associant grossesses uniques et multiples. Les résultats de cette étude sont certes rassurants, mais doivent être confirmées par d’autres séries

Effets de la durée d'ouverture des kits conventionnels de congélation/décongélation des blastocystes sur leur survie puis implantation

National audienc

Evolution des paramètres spermatiques d'hommes fertiles de 1976 à 2010

National audienc

Obstetric and perinatal outcomes of singletons after single blastocyst transfer: is there any difference according to blastocyst morphology?

A strong correlation between blastocyst morphology and implantation has been shown by many studies. The consequences and effects of assisted reproductive techniques on children's short and long-term health have always been a source of discussion. The obstetric and perinatal outcome of singletons according to blastocyst morphology has rarely been evaluated. The aim of this observational study is to determine whether a relationship exists between blastocyst morphology and obstetric and perinatal outcomes. A total of 799 singleton clinical pregnancies were analysed after transfer of a single fresh blastocyst on day 5 between 2006 and 2013. Blastocysts were divided into four groups based on their morphology on day 5: group 1 = good morphology blastocysts; group 2 = fair morphology blastocysts; group 3 = poor morphology blastocysts and group 4 = early (B1/B2) blastocysts. Obstetric and perinatal outcomes were compared between the four groups. After adjustment for some confounding variables, main obstetric and perinatal outcomes after transfer of blastocysts with poor morphological characteristics were not associated with increased adverse obstetric and perinatal events. Sex ratio was significantly higher in group 1 compared with groups 2, 3 and 4, and in Group 2 compared with Group 3 (P < 0.001) even after adjustment (P < 0.05)

Influence du tabagisme actif et passif sur le recrutement folliculaire et les résultats en FIV

National audienc

Treating women under 36 years old without top-quality embryos on day 2: a prospective study comparing double embryo transfer with single blastocyst transfer

 absen

Clinical outcome after insemination with donor sperm in patients with poor results in ICSI cycles

The introduction of intracytoplasmic sperm injection (ICSI) provided an effective treatment for infertile couples whose infertility was attributed to male factors. However, some of them face poor results after ICSI and subsequently use artificial insemination with donor sperm (AID). Only a few studies have reported on the clinical outcome of AID cycles after previous failed ICSI cycles, with contrasting results. The results reported here involve a cohort of 47 couples undertaking 175 AID cycles after 120 failed ICSI cycles for various reasons. Couples were allocated to two groups according to the availability of top quality embryos (TQE) in ICSI cycles. In our series, AID was successful for couples with and without TQE previously transferred in ICSI cycles, the live birth rate (LBR) per cycle being 20.0% and 13.3%, respectively. However, couples with TQE tended to succeed more rapidly than couples with poor quality embryos, with a higher cumulative LBR (68.0% versus 54.5%, respectively). These findings demonstrate that even couples with a history of unsuccessful ICSI cycles because of poor embryo quality are able to achieve high LBR after AID cycles. However, such couples have a lower cumulative LBR and are required to be more patient to achieve parenthood

Semen variation in a population of fertile donors: evaluation in a French centre over a 34-year period

Although it has been suspected that there is a decrease in semen quality over time, the results reported to date remain debatable because of methodological issues. The aim of the study reported here was to investigate the evolution of semen quality over time in a population of 1114 fertile candidates for sperm donation at CECOS, Tours, between 1976 and 2009. We investigated semen volume, sperm concentration, progressive motility, vitality, percentage of normal forms and multiple abnormalities index of the first ejaculate in this population. We did not find a decline in semen volume, whereas we observed a significant decrease in total sperm count (from 443.2 million in 1976 to 300.2 million in 2009), motility (from 64% in 1976 to 49% in 2009) and vitality (from 88% to 80%). Moreover, a significant decline in the percentage of normal forms was noted between 1976 and 1997 (from 67% to 26%) with a steady rise in the multiple abnormalities index between 1998 and 2009 (from 1.19 to 1.65). This study involving a population of fertile men from a restricted area revealed various degrees of decline in semen parameters over a period of 34 years. These findings will have to be compared with findings in other geographical areas

BMP4 stimulates development of ovine preantral follicles during ovarian cortex culture

International audienc