Design and fabrication of chemically robust three-dimensional microfluidic valves

A current problem in microfluidics is that poly(dimethylsiloxane) (PDMS), used to fabricate many microfluidic devices, is not compatible with most organic solvents. Fluorinated compounds are more chemically robust than PDMS but, historically, it has been nearly impossible to construct valves out of them by multilayer soft lithography (MSL) due to the difficulty of bonding layers made of non-stick fluoropolymers necessary to create traditional microfluidic valves. With our new three-dimensional (3D) valve design we can fabricate microfluidic devices from fluorinated compounds in a single monolithic layer that is resistant to most organic solvents with minimal swelling. This paper describes the design and development of 3D microfluidic valves by molding of a perfluoropolyether, termed Sifel, onto printed wax molds. The fabrication of Sifel-based microfluidic devices using this technique has great potential in chemical synthesis and analysis

Sites for Gamma-ray Astronomy in Argentina

We have searched for possible sites in Argentina for the installation of large air Cherenkov telescope arrays and water Cherenkov systems. At present seven candidates are identified at altitudes from 2500 to 4500 m. The highest sites are located at the Northwest of the country, in La Puna. Sites at 2500 and 3100 m are located in the West at El Leoncito Observatory, with excellent infrastructure. A description of these candidate sites is presented with emphasis on infrastructure and climatology.Comment: Submitted to Proceedings of "4th Heidelberg International Symposium on High Energy Gamma-Ray Astronomy 2008

Chandra and Hubble Study of a New Transient X-ray Source in M31

We present X-ray and optical observations of a new transient X-ray source in M31 first detected 23-May-2004 at R.A.=00:43:09.940 +/- 0.65'', Dec.=41:23:32.49 +/- 0.66''. The X-ray lightcurve shows two peaks separated by several months, reminiscent of many Galactic X-ray novae. The location and X-ray spectrum of the source suggest it is a low mass X-ray binary (LMXB). Follow-up HST ACS observations of the location both during and after the outburst provide a high-confidence detection of variability for one star within the X-ray position error ellipse. This star has $\Delta$B ~ 1 mag, and there is only a ~1% chance of finding such a variable in the error ellipse. We consider this star a good candidate for the optical counterpart of the X-ray source. The luminosity of this candidate provides a prediction for the orbital period of the system of 2.3$^{+3.7}_{-1.2}$ days.Comment: 17 pages, 3 figures, 4 tables, accepted for publication in Ap

Triploid Oysters In The Chesapeake Bay: Comparison Of Diploid And Triploid Crassostrea Virginica

Diploid and triploid Eastern oysters, Crassostrea virginica, were tested at 3 sites characterized by low on moderate salinity regimes in the Virginia part of the Chesapeake Bay from November 2005 through October 2007. Both diploid and triploid cultures were replicated 3 times by producing separate spawns from different broodstock. Ploidy had a generally consistent effect on the performance of C. virginica at the 3 test sites. At the end of the study, in October 2007, and across all sites, triploid oysters had lower cumulative mortality than diploids (-34%), and greater shell height (+25%), whole weight (+88%), and yield (+152%), as well as a higher proportion of market-size oysters (+114%) than diploids. Both diploids and triploids were similarly infected by Perkinsus marinas and, to a lesser extent, by Haplosporidium nelsoni. In a closer look, growth parameters (shell height growth, whole weight, yield, and percentage of marketable oysters) were always higher in triploids than in diploids regardless of the parental source, strongly supporting the superior advantage of triploids. Similar results were obtained for cumulative mortality, but to a lesser extent as a result of the large variation in mortality for both diploid and triploid cohorts among sites, as well as a significant site-by-cohort interaction. Our report is the first clear illustration of variation for the cumulative mortality exhibited among different spawns in triploids, and comes with the lesson that care must be taken in experiments in which the goal is to test the effect of ploidy on this trait. Our results support the notion that selective breeding programs to reduce mortality, coupled with triploid production to increase growth, can further optimize yield. The best-performing replicate spawn had 80% survival after 2.5 y, and reached an average shell height of 92 mm, weighing 142 g

A Compact X-ray Source and Possible X-ray Jets within the Planetary Nebula Menzel 3

We report the discovery, by the Chandra X-ray Observatory, of X-ray emission from the bipolar planetary nebula Menzel 3. In Chandra CCD imaging, Mz 3 displays hot (3-6x10^6 K) gas within its twin, coaxial bubbles of optical nebulosity, as well as a compact X-ray source at the position of its central star(s). The brightest diffuse X-ray emission lies along the polar axis of the optical nebula, suggesting a jet-like configuration. The observed combination of an X-ray-emitting point source and possible X-ray jet(s) is consistent with models in which accretion disks and, potentially, magnetic fields shape bipolar planetary nebulae via the generation of fast, collimated outflows.Comment: 12 pages, 3 figures; to appear in Astrophysical Journal (Letters

Optical Constraints on an X-ray Transient Source in M31

We have detected a transient X-ray source in the M31 bulge through a continuing monitoring campaign with the Chandra ACIS-I camera. The source was detected at R.A.=00:42:33.428 +/- 0.11'', Dec.=+41:17:03.37 +/- 0.11'' in only a single observation taken 2004 May 23. Fortuitous optical HST/ACS imaging of the transient location prior to the X-ray outburst, along with follow-up HST/ACS imaging during and after the outburst, reveals no transient optical source brighter than B (equivalent) = 25.5. The location of the source and its X-ray properties suggest it is a low mass X-ray binary (LMXB). Assuming the transient is similar to many Galactic X-ray novae, the X-ray luminosity of (3.9 +/- 0.5) X 10$^{37}$ erg s$^{-1}$ and the upper-limit on the optical luminosity provide a prediction of <1.6 days for the orbital period of the binary system.Comment: 15 pages, 3 figures, accepted for publication in Ap

A Soft X-ray Transient in the M31 Bulge

We have examined a probable soft X-ray transient source in the M31 bulge at R.A.=0:42:41.814 +/- 0.08", Dec. = 41:16:35.86 +/- 0.07". On the three occasions we observed the source, its spectrum was soft (kT_{in} ~1 keV). The brightest detection of the source was 2004 July 17 with a 0.3-7 keV luminosity of ~5 X 10^{37} erg/s. The only previous detection of the source was in 1979 by the Einstein observatory. The multiple detections over 25 years suggest the duty cycle of the source is in the range 0.02-0.06. Coordinated HST/ACS imaging before, during, and after the outburst revealed no variable optical source within the position errors of the X-ray source. The optical data place a firm upper limit on the brightness of the counterpart of the X-ray outburst of B>24.7, suggesting the binary has a period <5.2 days. The X-ray spectrum and lack of bright stars at the source location indicate the source was a soft transient event occurring in a low-mass X-ray binary, making this source a good black hole candidate in M31.Comment: 18 pages, 4 tables, 3 figures, accepted for publication in Ap

A Synoptic X-ray Study of M31 with the Chandra-HRC

We have obtained 17 epochs of Chandra High Resolution Camera (HRC) snapshot images, each covering most of the M31 disk. The data cover a total baseline of 2.5 years and contain a mean effective exposure of 17 ks. We have measured the mean fluxes and long-term lightcurves for 166 objects detected in these data. At least 25% of the sources show significant variability. The cumulative luminosity function (CLF) of the disk sources is well-fit by a power-law with a slope comparable to those observed in typical elliptical galaxies. The CLF of the bulge is a broken power law similar to measurements made by previous surveys. We note several sources in the southwestern disk with L_X > 10^{37} erg/s . We cross-correlate all of our sources with published optical and radio catalogs, as well as new optical data, finding counterpart candidates for 55 sources. In addition, 17 sources are likely X-ray transients. We analyze follow-up HST WFPC2 data of two X-ray transients, finding F336W (U-band equivalent) counterparts. In both cases, the counterparts are variable. In one case, the optical counterpart is transient with F336W = 22.3 +/- 0.1 mag. The X-ray and optical properties of this object are consistent with a ~10 solar mass black hole X-ray nova with an orbital period of ~20 days. In the other case, the optical counterpart varies between F336W = 20.82 +/- 0.06 mag and F336W = 21.11 +/- 0.02 mag. Ground-based and HST observations show this object is bright (V = 18.8 +/- 0.1) and slightly extended. Finally, the frequency of bright X-ray transients in the M31 bulge suggests that the ratio of neutron star to black hole primaries in low-mass X-ray binaries (NS/BH) is ~1.Comment: 68 pages (27 text), 8 tables, 16 figures, 1 appendix, accepted by ApJ; accepted version contains reorganized text, new tables and figures, and updated result

Tidal Disruption of a Star By a Black Hole : Observational Signature

We have modeled the time-variable profiles of the Halpha emission line from the non-axisymmetric disk and debris tail created in the tidal disruption of a solar-type star by a million solar mass black hole. Two tidal disruption event simulations were carried out using a three dimensional relativistic smooth-particle hydrodynamic code, to describe the early evolution of the debris during the first fifty to ninety days. We have calculated the physical conditions and radiative processes in the debris using the photoionization code CLOUDY. We model the emission line profiles in the period immediately after the accretion rate onto the black hole became significant. We find that the line profiles at these very early stages of the evolution of the post-disruption debris do not resemble the double peaked profiles expected from a rotating disk since the debris has not yet settled into such a stable structure. As a result of the uneven distribution of the debris and the existence of a ``tidal tail'' (the stream of returning debris), the line profiles depend sensitively on the orientation of the tail relative to the line of sight. Moreover, the predicted line profiles vary on fairly short time scales (of order hours to days). Given the accretion rate onto the black hole we also model the Halpha light curve from the debris and the evolution of the Halpha line profiles in time.Comment: 20 pages, 9 figures, to appear in ApJ, 1 August 2004 issue; mpeg simulations of tidal disruption available at http://www.astro.psu.edu/users/tamarab/tdmovies.htm