Uma tentativa de cultivo de rotavírus humano em cultura de leucócitos humano (Nota Prévia)

Experimentos preliminares da permissividade de cultura de leucócitos humanos estimulados com mitógeno frente a infecção pelo rotavirus humano foram realizados por microscopia eletrônica. Observamos que, células mo-nonucleadas, mantidas em cultura, após estimulação com fitohemaglutinina (PHA) colhidas 36 horas pós-infecção apresentavam muitas partículas virais no citoplasma. Verificamos, também, muitas partículas virais associadas a fragmentos celulares, várias células em degeneração e alguns linfócitos pequenos intactos. Não presenciamos partículas virais em células colhidas previamente (12 e 24 horas p.i.) e nas culturas controle (sem tratamento com PHA). Sugerimos que o rotavirus humano pode se replicar em culturas de leucócitos humanos estimulados com PHA

A proposal of sensitive indicators of the rehabilitation nursing care of people in the surgical process, to be included in the ontology of aging

Given the complex surgical scenario observed today, it is necessary to change the urgent definition of a specific ontology, especially in terms of aging. The interventions of the Specialist Nurse in Rehabilitation Nursing to the person in the surgical process are primordial for the maintenance of the functional capacities, prevention of complications and impediment of incapacities. However, it is necessary to provide individualized and fostered care in a practice of excellence, and the consequent organized reading of records of an aging ontology for decision making on rehabilitation indicators. Method: a systematic review of the literature was carried out using the EBSCO host (MEDLINE with Full Text, CINAHL Plus with Full Text and MedicLatina), using the PI [C] O method, and 11 articles were selected. Results: 41 indicators were identified to be included in the ontology classes of aging: pain control, patient and family education, support and communication, reduction of postoperative complications, gain of functionality, restoration of physical function, mobility, multidisciplinary intervention and the frequency of interventions. Conclusion: it is considered that it was imperative to identify sensitive indicators for rehabilitation nursing care, based on scientific evidence and thus provide an opportunity for nurses to reflect on their daily practices and conduct their actions towards excellence in care, to propose a set of 41 classes internationally on the ontology of aging

Contributions to the ontology of aging, the sensitive indicators of rehabilitation nursing care, in terms of self-care, in people with respiratory disorders

With the increase in the average life expectancy, the appearance of chronic diseases and, in particular, in the respiratory forum and its disadvantages in the autonomy and self-care of patients, brought an important paradigm for health care and, in particular, rehabilitation nursing, with implications for the development of ontologies of aging. Thus, it is imperative to systematize them in scientifically measurable indicators to demonstrate the benefit that rehabilitation nursing brings to improve patients' quality of life, thus ensuring excellence. Each area of scientific health should contribute to the construction of aging ontology. Objective: to identify indicators sensitive to nursing care of rehabilitation, in terms of self-care, in relation to those with respiratory pathology, to be integrated into the ontology of aging. Methods: A systematic review of the literature was performed using the EBSCO (full-text MEDLINE, CINAHL, Full-Text Plus, British Nursing Index), using the PI [C] O method with 6 emergent articles. Results: A total of 20 indicators were identified, including: Ability to perform activities, increase physical and functional independence, symptom management, reduction of complications, increase in quality of life, which can be allocated as a proposal for classes of the aging ontology. Conclusion: Knowledge of indicators sensitive to nursing care recognition of the importance of rehabilitation nursing in increasing self-care and autonomy for people with respiratory pathology, and research in this area is fundamental for its effectiveness and efficiency, and its integration in the ontologies of aging is fundamental

A global simulation for laser driven MeV electrons in 50μm50\mu m-diameter fast ignition targets

The results from 2.5-dimensional Particle-in-Cell simulations for the interaction of a picosecond-long ignition laser pulse with a plasma pellet of 50-$\mu m$ diameter and 40 critical density are presented. The high density pellet is surrounded by an underdense corona and is isolated by a vacuum region from the simulation box boundary. The laser pulse is shown to filament and create density channels on the laser-plasma interface. The density channels increase the laser absorption efficiency and help generate an energetic electron distribution with a large angular spread. The combined distribution of the forward-going energetic electrons and the induced return electrons is marginally unstable to the current filament instability. The ions play an important role in neutralizing the space charges induced by the the temperature disparity between different electron groups. No global coalescing of the current filaments resulted from the instability is observed, consistent with the observed large angular spread of the energetic electrons.Comment: 9 pages, 6 figures, to appear in Physics of Plasmas (May 2006

Uso de Geotecnologias na detecção de potenciais Áreas de Preservação Permanente e Reserva Legal na Região Administrativa Central do Estado de São Paulo.

Our objective in this work was to estimate, using geoprocessing techniques, the amount of permanent preservation areas (APPs) at the rural areas of the cities that are part of the Central Administrative Region of the State of São Paulo in the year 2016, to verify its adequacy to the New Brazilian Forest Code and to estimate, by means of land use and cover classification, the environmental liabilities for legal reserve (RL) areas in the region's cities. We additionally detected sugarcane crops in terrains with slope degrees higher than 12% and which will no longer be used for crops, in accordance with law decree 47.700/SP, and may therefore indicate areas which are viable for use as RLs, thus helping reduce the region's environmental liabilities. Our results show that the region contains 30.492,6 ha of environmental liabilities in APP areas and 27,493.6 ha in RL areas. Current sugarcane areas with potential to become RLs comprise 35,630.6 ha. The APP and RL areas are unevenly distributed across the region. Some cities showed no environmental liabilities, whereas others could reconcile or reduce theirs by 14.5% to 79.5% by using the areas that will no longer be used for sugarcane crops

Expansão da cafeicultura na porção leste do estado de São Paulo.

Our aim in this work was to map the main land-use and land-cover changes at 25 cities located at the watersheds of the Mogi-Guaçu and Pardo rivers over the last 27 years. We focused on coffee and sugarcane crops at these region, known as Mogiana Paulista. We updated the sugarcane crop areas to the year 2015 using maps produced by the Canasat project. For land-use and land-cover classification of these 25 cities in 2015 we used a Google Earth image file in a GIS environment. The method we used was on-screen visual image interpretation of high-spatial-resolution images, which were later compared to data from 1988. Along the 27- year period, coffee crops expanded approximately 80%, from 67.3 thousand to 123.8 thousand hectares. The 25 cities which are the main coffee producers at Mogiana Paulista showed concentration of coffee crops, which make up a total of approximately 111 thousand hectares. Both coffee and sugarcane may still advance onto parts of these cities' areas that are currently used as pastures, which the land-cover that loses more areas. Pastures still occupy 322 thousand hectares, or about 30% of the area of these 25 cities, and 252 thousand of these hectares feature slope degrees which are adequate for the mechanized harvesting of both sugarcane and coffee

Beam loading in the nonlinear regime of plasma-based acceleration

A theory that describes how to load negative charge into a nonlinear, three-dimensional plasma wakefield is presented. In this regime, a laser or an electron beam blows out the plasma electrons and creates a nearly spherical ion channel, which is modified by the presence of the beam load. Analytical solutions for the fields and the shape of the ion channel are derived. It is shown that very high beam-loading efficiency can be achieved, while the energy spread of the bunch is conserved. The theoretical results are verified with the Particle-In-Cell code OSIRIS.Comment: 5 pages, 2 figures, to appear in Physical Review Letter

Photodisintegration of the triton with realistic potentials

The process $\gamma + t \to n + d$ is treated by means of three-body integral equations employing in their kernel the W-Matrix representation of the subsystem amplitudes. As compared to the plane wave (Born) approximation the full solution of the integral equations, which takes into account the final state interaction, shows at low energies a 24% enhancement. The calculations are based on the semirealistic Malfliet-Tjon and the realistic Paris and Bonn B potentials. For comparison with earlier calculations we also present results for the Yamaguchi potential. In the low-energy region a remarkable potential dependence is observed, which vanishes at higher energies.Comment: 16 pages REVTeX, 8 postscript figures included, uses epsfig.st