As caracter?sticas da execu??o da gest?o ambiental pela Secretaria do Meio Ambiente do munic?pio de Cajazeiras-PB

Entendendo a dualidade existente entre a quest?o da utiliza??o e a preserva??o dos recursos naturais, assim como a import?ncia da realiza??o de estudos e pesquisas relacionados a essa tem?tica, o presente trabalho tem o escopo de verificar as caracter?sticas da execu??o da Gest?o Ambiental no munic?pio de Cajazeiras-PB, tendo em vista a necessidade de conhecer as legisla??es que subsidiam as a??es de vigil?ncia ambiental no munic?pio, buscando identificar aspectos relacionados as infra??es cometidas e suas respectivas penalidades com o intuito de constatar se s?o realizadas a??es de puni??o ao processo de degrada??o ambiental. Deste modo, foi realizada uma pesquisa descritiva de car?ter quantitativo, por meio de uma revis?o bibliogr?fica e documental em que coleta de dados foi feita atrav?s da aplica??o de um question?rio atrav?s da utiliza??o da Escala de Likert e an?lise dos dados realizada conforme verifica??o das informa??es coletadas

A Marketplace-based Approach to Cloud Network Slice Composition Across Multiple Domains

Cloud network slicing can be defined as the process that enables isolated end-to-end and on-demand networking abstractions, which: (a) contain both cloud and network resources, and (b) are independently controlled, managed and orchestrated. This paper contributes to the vision of the NECOS project and relevant platform, that aim to address the limitations of current cloud computing infrastructures to accomplish the challenging requirements of the slicing approach. The NECOS platform implements the Slice-as-a-Service model, enabling the dynamic creation of end-to-end (E2E) slices from a set of constituent slice parts contributed from multiple domains. A challenging issue is to define the facility that implements dynamic slice resource discovery, aligned to the requirements of the slice owner or tenant, over different infrastructure providers. Here, we propose a Marketplace-based approach implementing relevant federated interactions for the resource discovery and we detail its architecture, workflows, and information model. We also present its initial implementation details and provide both quantitative and qualitative experimental results validating its main operation

Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity

Brazil is the largest producer and consumer of common bean (Phaseolus vulgaris L.), which is the most important source of human dietary protein in that country. This study assessed the genetic diversity and the structure of a sample of 279 geo-referenced common bean landraces from Brazil, using molecular markers. Sixty-seven microsatellite markers spread over the 11 linkage groups of the common bean genome, as well as Phaseolin, PvTFL1y, APA and four SCAR markers were used. As expected, the sample showed lower genetic diversity compared to the diversity in the primary center of diversification. Andean and Mesoamerican gene pools were both present but the latter gene pool was four times more frequent than the former. The two gene pools could be clearly distinguished; limited admixture was observed between these groups. The Mesoamerican group consisted of two sub-populations, with a high level of admixture between them leading to a large proportion of stabilized hybrids not observed in the centers of domestication. Thus, Brazil can be considered a secondary center of diversification of common bean. A high degree of genome-wide multilocus associations even among unlinked loci was observed, confirming the high level of structure in the sample and suggesting that association mapping should be conducted in separate Andean and Mesoamerican Brazilian samples

A Mitochondrial Kinase Complex Is Essential to Mediate an ERK1/2-Dependent Phosphorylation of a Key Regulatory Protein in Steroid Biosynthesis

ERK1/2 is known to be involved in hormone-stimulated steroid synthesis, but its exact roles and the underlying mechanisms remain elusive. Both ERK1/2 phosphorylation and steroidogenesis may be triggered by cAMP/cAMP-dependent protein kinase (PKA)-dependent and-independent mechanisms; however, ERK1/2 activation by cAMP results in a maximal steroidogenic rate, whereas canonical activation by epidermal growth factor (EGF) does not. We demonstrate herein by Western blot analysis and confocal studies that temporal mitochondrial ERK1/2 activation is obligatory for PKA-mediated steroidogenesis in the Leydig-transformed MA-10 cell line. PKA activity leads to the phosphorylation of a constitutive mitochondrial MEK1/2 pool with a lower effect in cytosolic MEKs, while EGF allows predominant cytosolic MEK activation and nuclear pERK1/2 localization. These results would explain why PKA favors a more durable ERK1/2 activation in mitochondria than does EGF. By means of ex vivo experiments, we showed that mitochondrial maximal steroidogenesis occurred as a result of the mutual action of steroidogenic acute regulatory (StAR) protein –a key regulatory component in steroid biosynthesis-, active ERK1/2 and PKA. Our results indicate that there is an interaction between mitochondrial StAR and ERK1/2, involving a D domain with sequential basic-hydrophobic motifs similar to ERK substrates. As a result of this binding and only in the presence of cholesterol, ERK1/2 phosphorylates StAR at Ser232. Directed mutagenesis of Ser232 to a non-phosphorylable amino acid such as Ala (StAR S232A) inhibited in vitro StAR phosphorylation by active ERK1/2. Transient transfection of MA-10 cells with StAR S232A markedly reduced the yield of progesterone production. In summary, here we show that StAR is a novel substrate of ERK1/2, and that mitochondrial ERK1/2 is part of a multimeric protein kinase complex that regulates cholesterol transport. The role of MAPKs in mitochondrial function is underlined

Genetic diversity in cultivated carioca common beans based on molecular marker analysis

A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats – SSRs and amplified fragment length polymorphisms – AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger’s modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm