Infezione sistemica da Salmonella Arizona: discussione di un raro caso chirurgico.

Salmonella arizona enteritis has been described in patients resident in the southern states of the USA and in Mexico, whereas in Europe it is rarer. The virulence of this bacillus is, however, still little known and we have few descriptions of severe systemic infections, which are all present in patients with immune system impairment. Only two cases have been reported in Italy where the infection has occurred as severe sepsis with the pathogenic agent being isolated in the blood. Here we report what is, on the basis of our knowledge, the third case in Italy of a systemic Salmonella arizona infection

Development and validation of a solid-phase extraction method coupled to high-performance liquid chromatography with ultraviolet-diode array detection for the determination of sulfonylurea herbicide residues in bovine milk samples

This study proposes a fast, simple and sensitive liquid chromatography diode array detector (LC/UV-DAD)-based method for the simultaneous determination of eight sulfonylurea herbicides (bensulfuron methyl, chlorsulfuron, metsulfuron methyl, primisulfuron methyl, rimsulfuron, thifensulfuron methyl, triasulfuron and tribenuron methyl) in bovine whole milk at concentrations lower than the default limit of 0.01mgkg-1 allowed by current legislation (Regulation EC/396/2005 and following Annexes). An effective one-step solid phase extraction (SPE) and clean up procedure was defined with use of Chem Elut cartridges, providing good recoveries for all the analytes tested and with no matrix effects affecting method accuracy. Separation of herbicides was obtained on a C18 column by acetonitrile- water gradient elution. Method validation has been performed according to European Commission Decision 2002/657/EC criteria, in terms of linearity, recovery, precision, specificity, decision limit (CC??) and detection capability (CC??). Typical recoveries ranged between 78.4% and 99.7%, at the maximum residue limits (MRLs) levels established by Regulation EC/396/2005, with relative standard deviations (RSD) no larger than 10%

Multiresidue determination of nicotinoid insecticide residues in drinking water by liquid chromatography with electrospray ionization mass spectrometry

A multianalyte method has been developed for the confirmation and quantification of four neonicotinoids, acetamiprid, imidacloprid, thiacloprid and thiamethoxam in drinking water. These insecticides were extracted from water by solid-phase extraction (SPE) LiChrolut EN. Analytes’ determination and quantification were performed by liquid chromatography–mass spectrometry (LC–MS). Extraction efficiency experiments demonstrated the ability of this method to extract nicotinoids from water samples. Confirmatory analysis was carried out by LC-electrospray ionization–mass spectrometry (LC-ESI–MS) instrumentation equipped with a single-quadrupole mass filter. MS data acquisition was performed by two-ion selected ion monitoring (SIM) program. Average recoveries of the four analytes from drinking water samples were in the range of 95 and 104% with relative standard deviations (R.S.D.) < 20%. The limit of quantification (LOQ) for each of the four insecticides was 0.03 microgL−1