THE ANTIMYCOTIC EFFECT OF SOME BEE PRODUCTS AND VEGETAL EXTRACTS ON GENUS MALASSEZIA FUNGI

For both antimicrobial and antimycotic therapy, the researches of the latest years are mentioning an increased resistance of the bacterial and fungal strains to these substances. A natural alternative to classical therapy was aimed, so 14 Malassezia pachydermatis strains isolated from dogs suffering with dermatitis and otitis in Cluj-Napoca were tested regarding the capacity of some bee products to inhibit their “in vitro” development. From the products tested, the best results were registered for propolis tincture, polioel 3 and royal jelly. The media of the diameter inhibit area in mm, was of 22,21 mm for propolis tincture, 14,92 mm for polioel 3 and respectively of 8,78 mm for royal jell

Anatomical and Histopathological Researches in Experimental Infections with Prototheca Algae in Mice

In order to realize this study, three BALB mice groups were used: one inoculated with P. zopfii, one with P. wickerhamii suspension and one control group. Each group was constituted of twelve animals, 2 months old, with an approximate body mass of 20 g. Both Prototheca species: one referent strain Prototheca wickerhamii (RE-4608014 ATCC 16529) and one Prototheca zopfii isolate (from cow mastitic milk sample), were cultured in glucose broth medium, 48 hours, at 37ºC. Except control group, each animal was i.p. inoculated with 0,1 ml algal suspension. Although any clinical disease sign was not observed in 10, 15 and 20 days from the beginning of the experiment for both inoculated groups, four mice were slaughted. Microscopic lesions caused by Prototheca algae were represented by granuloma and piogranuloma found in different internals as confirmed by identification of organism by staining with periodic acid-Schiff (PAS) and culture. So appreciated illness severity we utilized a score system in order to obtained a lesional systemic score for each animal. We appreciated: limph nodes reaction, the presence or absence of granuloma in skin, liver, pancreas, diaphragm, kidney, spleen and intestine. The data were statistically process using “t” test. In group inoculated with P. zopfii the lesions were more severs than those evidenciated in P. wickerhamii infected group

EPIDEMIOLOGIC STUDY OF DERMANYSSUS GALLINAE (ACARI:DERMANYSSIDAE) INFESTATION IN BIRDS, FROM THREE LOCALITIES ON CLUJ AREA

The researches were made during June 2005-April 2006, on birds from exthensive system, from 3 locations on Cluj county: Apahida (location 1), Ocna-Dej (location 2) and Floreşti (location 3), adult and young birds were examinated. In each locality were examinated sistematicaly in each season, 20 private yards. The medium prevalence of D. gallinae infestation, during one year, had the bigest value in the locality Apahida ( 72,5 % ), in Ocna-Dej was 61,25 %, and in Floresti the prevalence was 57,5 %. From the collected mites were isolated E.coli, B. cerreus, Pseudomonas spp., Staphylococcus spp., Streptococcus spp., Micrococcus spp. and Corynebacterium spp

SHEEP CUTANEOUS HIPERSENSITIVITY IN EXPERIMENTAL INFESTATION WITH LUCILIA SERICATA LARVAE

The experiment considered two groups of Romney March sheep, the first one experimentally blowfly infested, and a second noninfested witness group. The larvae L. sericata antigen and salina inoculation was tested measuring the thin skin respense. The most intense skin reaction appeared at 24 h from antigen testing.In table 1 are presented the group and skin t-student test results

LABORATORY DIAGNOSIS OPTIMIZING TESTS IN AMERICAN FULLBROAD

We examined 24 honey samples with sapling, and 4 of them(16,67%) contained Paenibacillus larvae. Bacteriological exam allowed us to isolate P. larvae after 3-5 days of inoculation period at 37ºC. On solid medium (glucose agar with 1‰ yolk of an egg 1/5 and BHI agar with 0,2 mg/l thiamine). In case of lichid growth, we isolated P. larvae after two days. The study of biochemical properties of P. larvae strains isolated from the four samples on API 20E galery confirmed that this belong to Paenibacillus larvae species subsp. larvae. Serological exam, trought VITA AFB Diagnostic Kit established the quick diagnostic (aproximatly 10 minutes), at the four samples witch were positive throught the classical bacteriological techniques, with new perspectives in optimising the diagnostics in American foulbrood

FLORESCENT IN SITU HYBRIDIZATION (FISH) METHOD OPTIMIZATION FOR RAPID DETECTION OF PROTOTHECA IN CLINICAL SAMPLES

Members of the genus Prototheca are aerobic, unicellular algae related to the green algae of the genus Chlorella, but without chlorophyll. They are resistant and ubiquitous and can be isolated from a great variety of environmental sources. Many species of Prototheca genus have been identified but only two P. zopfii and P. wickerhamii, are able to produced infection in humans and animals. Frequently P. zopfii is most incriminated into the pathologic process in animals, and P. wickerhamii in humans, but is still hard to establish a strict delimitation. By different animal species, the most sensitive seems to be the cows, in witch will evolve as enzootic mastitis, and dogs in witch some systemic infections are described. Majority of humans infections are produced by P. wickerhamii and were mostly described in patients with immunosuppressant disorders, chronic diseases or after intensive treatments with various antibiotics. In this study we report a FISH method optimization, for Prototheca, which can be used for rapid diagnosis both in humans as well as in animals protothecosis. Reliable and quick identification of P. zopfii and P. wickerhamii is important for accurate treatment and understanding their role in the pathogenesis of infections. Fluorescent in situ hybridization (FISH) of Prototheca algae with specific eukaryotic probe EUK 516 ACCA GAC TTG CCC TCC leads to a reduced time to identification. In clinical practice, FISH therefore can be used in situations in which quick identification is necessary for optimal treatment of the patient

CULTURE CHARACTERIZATION OF DERMATOPHYTES FROM GENUS TRICHOPFHYTON IN CATTLE

In domestic ruminants (calves, cattle) dermatophytoses (ringworm) are caused mainly by T. verrucosum and sometimes by T. mentagrophytes (1). These fungi develop in the superficial layer of the skin, nails, and hair.This skin disease is spread worldwide in cattle and is responsable for high economic losses in cattel farm

ANTIBACTERIAL EFFECT OF ALBIENS ALBA AND LAVANDULA AUGUSTIFOLIA OILY EXTRACTS ON VARIOUS BACTERIAL SPECIES

The aim of the study is to evaluate the antibacterial action of Albiens alba and Lavandula augustifolia oily extracts on bacterial species such as: Staphylococcus intermedius, Streptococcus pyogenes, Escherichia coli and Pseudomonas aeruginosa. Essential extracts of the two plants in 3 dilutions (20%, 10%, 1%) were used for each bacterial specie. Bacterial strains were isolated from dogs with dermatitis. For germs sensibility tests natibiograma by difusimethrical method was used, utilising a culture of 24 h with 2 optical density on MacFarland scale

ISOLATION AND CHARACTERIZATION OF AN AEROMONAS HYDROPHILA STRAIN IN A CRAP (CYPRINUS CARPIO) TOXEMIA FOCUS

Bacteriological exam performed in 7 diseased crap (Cyprinus carpio), from a private breeding in Satu-Mare county leaded to the isolation of a Aeromonas hydrophila strain from each individual in pure culture, in fact toxemia. Specie identification was realized using API 20E biochemical system. A. hydrophila strain isolated was sensible to Enrofloxacin, Florfenicol, Oxitetracyclin and resistant to Ampicilin, Amoxicillin and Erythromycin. Therapy using Enrofloxacin, 20g/10kg food, administrated for 7 days 2 times a day leaded to the improvement of the general health status and cutaneous lesions healin

BIOCHEMICAL TESTS USED FOR IDENTIFICATION OF BORDETELLA BRONCHISEPTICA

18 isolates from pigs suspected to be B. bronchiseptica were submited to citrate, oxidase, catalase, production of H2S and indole, acid production from glucose, lactose, manose, inositol, sorbitol, rhamnose, sucrose, melobiose and arabinose biochemical tests. Urease and motility tests were performed on MIU culture media in both modulating and non-modulatig conditions. The obtained biochemical profile (positive reactions for oxidase, catalase production; urease production and motility in modulating conditions; utilisation of the sodium citrate as a single carbon source; negative reactions for acid production in the slant or butt of TSI agar; H2S production in the TSI agar; acid production from manose, inositol, sorbitol, rhamnose, sucrose, melobiose and arabinose in the column of OF culture media; urease and mobility in MIU culture media in non- modulating conditions) matched 100% the biochemical profile of B. bronchiseptica witch combined with the fact that all strains had tipical B. bronchiseptica colonial morphology on MacConkey agar allowed us to identify the studied strains as B. bronchiseptica