Occurrence of Ancylostoma in dogs, cats and public places from Andradina city, São Paulo state, Brazil

The aim of this study was to determine the frequency and intensity of Ancylostoma spp. in 33 dogs and 52 cats by means of coproparasitological examinations and parasitological necropsy, and assess the presence of contaminated feces with eggs of that parasite in public places of Andradina Municipality, São Paulo State, Brazil. Willis-Mollay and Sedimentation methods indicated Ancylostoma spp. eggs in 87.8% (29/33) dogs and 94.2% (49/52) cats. The species A. caninum and A. braziliense were found in 63.6% (21/33) and 30.3% (10/33) of dogs, respectively. Considering cats, 67.3% (35/52) were parasitized by A. braziliense, 21.1% (11/52) by A. caninum, and 9.6% (5/52) by A. tubaeforme. Forty-two canine fecal samples were collected from public environments, including 23 squares/gardens and 19 streets/sidewalks. Positive samples for Ancylostoma spp. accounted for 64.3% (27/42); squares/gardens had 60.9% (14/23) positive samples, and streets and sidewalks, 68.4% (13/19). No association was observed between the number of Ancylostoma spp parasites and age, sex and breed of the animals and also the ratio of EPG counts and the parasitic intensity observed at necropsy (p > 0.05). Based on the high occurrence of hookworm in dogs and cats in this study, the treatment with anti helminthics are needed even in those animals with negative stool tests, besides adopting control of the number of animals in public places, in order to decrease the likelihood of environmental contamination, since this parasite represents a potential hazard to human and animal health

Polysaccharide-based Membranes Loaded With Erythromycin For Application As Wound Dressings

In this study, the antibiotic erythromycin (Ery) was incorporated into chitosan (Ch)-alginate (A) and Ch-xanthan (X) membranes with the aim of using them as bioactive wound dressings. Drug incorporation was performed by direct addition (DA) to the polysaccharide mixture and by membrane impregnation in solution (IS). A higher incorporation efficiency was obtained for DA, but higher amounts of drug were loaded into membranes by the IS method (maxima approximate to 2.1 and 0.7 g/g for Ch-X and Ch-A, respectively) because the initial concentration of drug could be higher than that in the DA method. Ery release in phosphate-buffered saline was slow, reaching about 12 and 32 mg of drug/g of membrane in 60 h for Ch-X and 4 and 16 mg/g for Ch-A by the DA and IS methods, respectively. With formulations prepared with IS, the required therapeutic dosage was reached within 60 h, whereas for those incorporating the drug by DA, prolonged use would be required. Both membrane types behaved as drug reservoirs, providing continuous antibiotic release to the wound site. Formulations with higher drug contents showed effective antibacterial activity against two species of bacteria commonly found in skin lesions, Staphylococcus aureus and Pseudomonas aeruginosa, and were thus potentially capable of protecting the wound site from bacterial attack. (C) 2016 Wiley Periodicals, Inc.13

Adequacy of human milk viscosity to respond to infants with dysphagia: experimental study

Neonatal nutrition is an important subject in health in the short, medium and long term. In preterm newborns, nutrition assumes a predominant role for the child's overall development. Babies with uncoordinated swallowing or respiration may not have the necessary oral abilities to suck the mother's breast and will need to implement different feeding practices; one of them is changing the consistency of the milk offered. OBJECTIVES: Determine viscosity variations of untreated human and pasteurized milk without and with thickening to adapt the diet to the needs of dysphagic infants hospitalized in the Neonatal Intensive Cara Unit (NICU). MATERIAL AND METHODS: The authors altered the viscosity of natural infant powdered milk and, after thickening, determined and adopted a thickening standard for human milk. Untreated human and pasteurized milk was thickened in concentrations of 2%, 3%, 5% and 7% and the viscosity were determined every 20 minutes for a period of 60 minutes at a temperature of 37ºC. RESULTS: The infant lactose formula thickened at concentrations of 2% and 3% produced viscosities of 8.97cP and 27.73 cP, respectively. The increases were significantly different after 1 hour. Inversely, untreated human milk at 2%, 3%, 5% and 7% produced diminished viscosity over time; the changes were more accentuated in the first 20 minutes. In pasteurized human milk, the 2% concentration had no variation in viscosity, but with the 3%, 5% and 7% concentrations, there was a significant decrease in the first 20 minutes with stability observed in the subsequent times. CONCLUSION: In powdered milk, the viscosity increases over time; the viscosity in human milk diminishes. The results point out the importance not only of considering the concentration of the thickener but also the time being administered after its addition to effectively treat dysphagic infants

A alimentação do prematuro por meio do copo Cup-feeding of premature newborn children

OBJETIVO: Quantificar a proporção de leite perdido na oferta da dieta com copo; o tempo de oferta e analisar se há relação do desperdício e o tempo gasto na ingesta com as variáveis: sexo, dias de vida, idade gestacional corrigida, tempo de sonda orogástrica, tempo de alimentação por via oral, estado de vigília e sinais de estresse. MÉTODOS: Participaram 20 recém-nascidos prematuros, 11 do sexo feminino e nove do masculino. A média de idade gestacional corrigida foi de 36 semanas e o peso na avaliação de 1668 gramas. Os bebês eram alimentados via oral por, no mínimo, dois dias. Seus prontuários foram analisados quanto ao sexo, idade gestacional, peso atual, dias de vida, tempo de alimentação por via oral e tempo de uso de sonda orogástrica para relacionar aos dados da oferta da dieta. Para a administração do leite, o recém-nascido foi posicionado segundo as normas da Organização Mundial de Saúde. O alimento foi ofertado utilizando-se copo descartável de 50 mililitros, uma gaze foi colocada como anteparo e o tempo da oferta mensurado. Posteriormente, a gaze foi pesada. RESULTADOS: Houve diferença estatisticamente significante entre os volumes aceito (81,5%) e desperdiçado (22,8%) de leite, com maior aceite para o sexo feminino e para os bebês que não apresentaram sinais de estresse. O tempo médio de ingestão da dieta foi de 416 segundos. CONCLUSÕES: Os recém-nascidos pré-termo do sexo feminino aceitaram maior volume de leite, e quanto menor a exposição aos fatores estressantes, maior o aceite da dieta. O tempo médio gasto para ingestão da dieta não foi influenciado pelas variáveis analisadas.<br>PURPOSE: To quantify the milk loss ratio and the length of cup-feeding, and to analyze whether the waste and time expended in ingestion are related to the variables: sex, days of life, corrected gestational age, period of time using orogastric tube, period of time receiving oral feeding, alertness, and signs of stress. METHODS: Twenty premature newborn children, 11 female and nine male, participated on this study. The mean corrected gestational age was 36 weeks, and mean weight during evaluation was 1668 grams. The babies were orally fed for, at least, two days. Their medical files were analyzed regarding sex, gestational age, current weight, days of life, period of time receiving oral feeding, and period of time using orogastric tube, in order to relate these data with cup-feeding offer. For cup-feeding, the newborns were positioned according to World Health Organization standards. The milk administration was carried out using disposable 50-milliliters cups; gauze was placed as a shield, and the length of offer was measured. Later, the gauze was weighed. RESULTS: There was a significant statistical difference between the volumes of accepted (81.5%) and wasted (22.8%) milk, with more acceptance for female newborns and for the babies who did not present signs of stress. The mean length of cup-feeding was 416 seconds. CONCLUSION: Female preterm newborns accepted grater milk volume, and the lesser exposition to stressing factors the bigger the acceptance of milk. The mean length of ingestion was not influenced by the variables analyzed