Anotação funcional da família gênica Dof no genoma da videira.

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Genome-wide identification of genes encoding DOF transcription factors in grapevine.

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Genome-wide identification of genes encoding dof transcription factors in grapevine.

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Análise global da expressão de genes Dof em videira.

Neste trabalho é caracterizada a família gênica Dof em videira (Vitis vinifera L.), a qual é composta por 25 genes. A busca por domínios conservados foi realizada utilizando-se o programa MEME suite v.4.9.0. Estudos filogenéticos foram conduzidos comparando 163 sequências Dof de videira, álamo, Arabidopsis thaliana e tomate com proteínas Dof de função conhecida previamente descritos na literatura

Análise funcional de genes de importância agronômica em macieira.

Neste estudo piloto, o gene repórter GFP foi clonado e testado em plantas de macieira para avaliar a utilização da tecnologia TraitUP

Differential transcriptional profiles of dormancy-related genes in apple buds

The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees.DOI 10.1007/s11105-013-0690-

Identificação e análise de promotores de desidrinas no genoma da macieira.

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Dormancy-related transcript accumulation of the dehydrin gene family in apple buds.

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Differential gene expression of two apple cultivars with contrasting chilling requirement.

Temperate fruit crops are of great economic importance worldwide, and their production depends on developmental processes, mainly the shift from juvenile to reproductive phase, dormancy transitions and flowering.Resumo