Comparative morphological, anatomical, ecological and chemical studies on endemic Satureja parnassica subsp. sipylea from Turkey

Satureja. parnassica Heldr.et Sart. subsp. sipylea P.H. Davis is a subspecies endemic to Turkey. The anatomical, morphological, ecological and chemical features of S. parnassica subsp. sipylea have been investigated. Plant samples were collected from different regions in Turkey. The morphological features of various organs of the plant such as leaf and flower are described in detail. In anatomical studies, transverse sections of the plant stem and leaf have been examined and supported by illustration and photographs. Ecological studies provide information about the physical and chemical structure of soil types in Spil Mountain and Marmara Island. The chemical composition of the essential oil of aerial parts of the plant was examined

Hepatoblast and mesenchymal cell-specific gene-expression in fetal rat liver and in cultured fetal rat liver cells

The aim of this study was to determine whether passaged rat fetal liver cells are functional hepatoblasts. Hepatocyte/hepatoblast- and liver myofibroblast-gene-expressions were studied in adult and fetal rat liver tissues as well as in primary and passaged cultures of isolated rat fetal liver cells at both the mRNA and protein level. Desmin- and Alpha-Smooth Muscle Actin (SMA)-positive cells were located in the walls of liver vessels, whereas Desmin-positive/SMA-negative cells were distributed within the liver parenchyma. Primary cultures contained Prox1-positive hepatoblasts, Desmin/SMA-positive myofibroblasts and only a few Desmin-positive/SMA-negative cells. Albumin and alpha-fetoprotein (AFP) could be detected in the primary cultures and to a lesser extent after the first passage. The number of Desmin-positive/SMA-negative cells decreased with successive passage, such that after the second passage, only Desmin/SMA-positive cells could be detected. SMA-gene-expression increased during the passages, suggesting that myofibroblasts become the major cell population of fetal liver cell cultures over time. This observation needs to be taken into account, should passaged fetal liver cells be used for liver cell transplantation. Moreover it contradicts the concept of epithelial-mesenchymal transformation and suggests rather that selective overgrowth of mesenchymal cells occurs in culture

Altered regulation of Prox1-gene-expression in liver tumors

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Comparative morphological, anatomical, ecological and chemical studies on endemic Satureja parnassica subsp. sipylea from Turkey

Satureja. parnassica Heldr.et Sart. subsp. sipylea P.H. Davis is a subspecies endemic to Turkey. The anatomical, morphological, ecological and chemical features of S. parnassica subsp. sipylea have been investigated. Plant samples were collected from different regions in Turkey. The morphological features of various organs of the plant such as leaf and flower are described in detail. In anatomical studies, transverse sections of the plant stem and leaf have been examined and supported by illustration and photographs. Ecological studies provide information about the physical and chemical structure of soil types in Spil Mountain and Marmara Island. The chemical composition of the essential oil of aerial parts of the plant was examined

Non-invasive and high-throughput interrogation of exon-specific isoform expression.

Expression of exon-specific isoforms from alternatively spliced mRNA is a fundamental mechanism that substantially expands the proteome of a cell. However, conventional methods to assess alternative splicing are either consumptive and work-intensive or do not quantify isoform expression longitudinally at the protein level. Here, we therefore developed an exon-specific isoform expression reporter system (EXSISERS), which non-invasively reports the translation of exon-containing isoforms of endogenous genes by scarlessly excising reporter proteins from the nascent polypeptide chain through highly efficient, intein-mediated protein splicing. We applied EXSISERS to quantify the inclusion of the disease-associated exon 10 in microtubule-associated protein tau (MAPT) in patient-derived induced pluripotent stem cells and screened Cas13-based RNA-targeting effectors for isoform specificity. We also coupled cell survival to the inclusion of exon 18b of FOXP1, which is involved in maintaining pluripotency of embryonic stem cells, and confirmed that MBNL1 is a dominant factor for exon 18b exclusion. EXSISERS enables non-disruptive and multimodal monitoring of exon-specific isoform expression with high sensitivity and cellular resolution, and empowers high-throughput screening of exon-specific therapeutic interventions