6 research outputs found
Improving control in microbial cell factories: from single-cell to large-scale bioproduction
Bioprocess deviations are likely to occur at different operating scales, leading in most of the case to substrate deviation from main metabolic routes and impact product synthesis. Correlating qS and qP is of utmost importance for bioprocess observability and control and can be modeled actually by advanced metabolic flux models. However, if most of these models are able to make prediction about metabolic switches, they still do not incorporate deviation due to biological noise, i.e. phenotypic and genotypic heterogeneity. These limitations impair observability and thus the use of fundamental knowledge about biological network for practical application, i.e. metabolic engineering or bioprocess scale-up
Cold adaptation of tRNA nucleotidyltransferases: A tradeoff in activity, stability and fidelity
The Role of Charged Residues in the Structural Adaptation of Short-Chain Alcohol Dehydrogenase (SDR) from Thermophilic Organisms to High Temperatures
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis
Genomics of psychrophilic bacteria and archaea
Genomes are available for a wide range of psychrophilic bacteria and archaea. As of early 2017, approximately 130 cold-adapted species have genome sequences. Several studies complement this data with functional studies. In this review the cold adaptation traits of psychrophilic microorganisms are explored from a genome-centric point of view including surveys of traits across genomes. A broader view of psychrophiles in terms of growth rates amongst life on Earth explaining what a psychrophile represents is presented. Trait surveys, limited to the perspective of gene gain, reveal prevalence of genes demonstratively providing better growth at low temperature including compatible solute uptake and synthesis, antifreeze proteins and polyunsaturated fatty acids and investigate their functional relevance to psychrophily. This includes revealing prevalent antifreeze DUF3494-type proteins that occur in all domains of life but is limited to cold-adapted taxa and is absent in higher-temperature adapted life