Monolithic zirconia and digital impression: case report

The aim of this study is to present a clinical case of a full arch prosthetic rehabilitation on natural teeth, combining both digital work-flow and monolithic zirconi

Digital impression on transmucosal vertical neck® implants: evaluation of soft tissues stability

BACKGROUND: The literature indicates that 0.5 to 1.5 mm of gingival recession most often occurs within the first months after implant placement or abutment connection. The aim of this study is to test the validity of the new Biotype implant design (Vertical Neck®) to evaluate the effect of a concave transmucosal profile on the vertical stability of soft tissues at the facial aspect of dental implants, taking advantage of the benefits deriving from intraoral digital impression. METHODS: The study was carried out at the Department of Oral and Maxillofacial Sciences, Sapienza - University of Rome. A 45-years-old patient was selected. He didn’t show periodontal and systemic diseases. Mono edentulous sites were either in maxilla (1.6) and in mandible (3.6). After the sign of the informed consent form, according to the World Medical Declaration of Helsinki, the surgical phase was performed. Mini-invasive osteotome sinus floor elevation in atrophic maxilla was used to insert a transmucosal Vertical Neck® implant with 4.8 mm diameter and 8 mm length. In mandible was used a transmucosal Vertical Neck® implant with 4.1 mm diameter and 8 mm length. Six months later a digital impression was taken with an intraoral optical scanner (CS3500, Carestream Dental, Atlanta, GA, USA). A Simbiosi® Scan Body was applied on implants. Periapical radiographs were taken in order to show the right linkage between devices and implants. Straight titanium abutmentswere used and periapical radiographs were made to evaluate a correct marginal fit on implants. Two monolithic zirconia crowns were realized with a CAM system. RESULTS: No recession in soft tissue was observed. The gingival level remained stable at 12 months. Vertical Neck® implants have shown a good relationship with bone and soft tissues. Also the aesthetic goals have been reached. CONCLUSIONS: Biotype transmucosal Vertical Neck® implants have been projected to allow the placement of prosthetic border into an area of 2 mm of eight instead of predetermined point. Because of its intrinsic feature, the biological width is respected. Thanks to the management of the impression into a digital format (intraoral scan and CAD/CAM system), the final crowns resulted to be more accurate than with a traditional system; infact, common mistakes linked to clinical and laboratory process have been avoided

The non-euphoric phytocannabinoid cannabidivarin counteracts intestinal inflammation in mice and cytokine expression in biopsies from UC pediatric patients

Patients with ulcerative colitis (UC) using marijuana have been reported to experience symptomatic benefit. Cannabidivarin (CBDV) is a safe non-psychoactive phytocannabinoid able to activate and desensitize TRPA1, a member of the TRP channels superfamily, which plays a pivotal role in intestinal inflammation. Here, we have investigated the potential intestinal anti-inflammatory effect of CBDV in mice and in biopsies from pediatric patients with active UC. Colonic inflammation was induced in mice by dinitrobenzenesulfonic acid (DNBS). The effect of orally administered CBDV on macroscopic and microscopic damage, inflammatory parameters (i.e. myeloperoxidase activity, intestinal permeability and cytokine production) and faecal microbiota composition, was evaluated 3 days after DNBS administration. TRPA1 expression was studied by RT-PCR in inflamed colons of mice as well as in mucosal colonic biopsies of children with active UC, whose response to incubation with CBDV was also investigated. CBDV attenuates, in a TRPA1-antagonist sensitive manner, DNBS-induced signs of inflammation including neutrophil infiltration, intestinal permeability, and cytokine (i.e. IL-1\u3b2, IL-6 and the chemokine MCP-1) production. CBDV also alters the dysregulation of gut microbiota associated to colitis. Finally, CBDV lessens cytokine expression in colonic biopsies from pediatric patients with ulcerative colitis, a condition in which TRPA1 was up-regulated. Our preclinical study shows that CBDV exerts intestinal anti-inflammatory effects in mice via TRPA1, and in children with active UC. Since CBDV has a favorable safety profile in humans, it may be considered for possible clinical trials in patients with UC