Biochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe

The Schizosaccharomyces pombe mag1 gene encodes a DNA repair enzyme with sequence similarity to the AlkA family of DNA glycosylases, which are essential for the removal of cytotoxic alkylation products, the premutagenic deamination product hypoxanthine and certain cyclic ethenoadducts such as ethenoadenine. In this paper, we have purified the Mag1 protein and characterized its substrate specificity. It appears that the substrate range of Mag1 is limited to the major alkylation products, such as 3-mA, 3-mG and 7-mG, whereas no significant activity was found towards deamination products, ethenoadducts or oxidation products. The efficiency of 3-mA and 3-mG removal was 5–10 times slower for Mag1 than for Escherichia coli AlkA whereas the rate of 7-mG removal was similar to the two enzymes. The relatively low efficiency for the removal of cytotoxic 3-methylpurines is consistent with the moderate sensitivity of the mag1 mutant to methylating agents. Furthermore, we studied the initial steps of Mag1-dependent base excision repair (BER) and genetic interactions with other repair pathways by mutant analysis. The double mutants mag1 nth1, mag1 apn2 and mag1 rad2 displayed increased resistance to methyl methanesulfonate (MMS) compared with the single mutants nth1, apn2 and rad2, respectively, indicating that Mag1 initiates both short-patch (Nth1-dependent) and long-patch (Rad2-dependent) BER of MMS-induced damage. Spontaneous intrachromosomal recombination frequencies increased 3-fold in the mag1 mutant suggesting that Mag1 and recombinational repair (RR) are both involved in repair of alkylated bases. Finally, we show that the deletion of mag1 in the background of rad16, nth1 and rad2 single mutants reduced the total recombination frequencies of all three double mutants, indicating that abasic sites formed as a result of Mag1 removal of spontaneous base lesions are substrates for nucleotide excision repair, long- and short-patch BER and RR

Vibration Signature of Normal and Notched Tooth Gear Pump

Gear pumps are critical devices in many industrial applications. An unexpected failure of the gear pump may cause significant effect on its performance. Consequently, there will be economic losses. As a result of that, fault diagnosis in gears has been the subject of intensive research. Vibration analysis has been used as an effective tool in machines diagnosis and in machinery maintenance decisions. As a rule, an increased vibration level is a warning form before failure or breakdown. By measuring and analyzing the gear pump vibration, it is possible to determine both the nature and severity of the defect, and hence predict the machine’s failure. The vibration signal of a gear pump carries the signature of the fault in the gears, and early fault detection of the gear pump is possible by analyzing the vibration signal using different signal processing techniques. This paper presents, experimentally, the external gear pump signature for normal and faulty gear pumps at different rotational speeds (1080, 1200, and 1439rpm). The considered faults herein are two different notches on one of the pump teeth— small notch and large notch. The paper concludes that features of the vibration are different with the notch shape and the rotational speed. The amplitude of vibration increases by increasing both rotational speed and notch size

Testing alternative tectonic models for the Permian-Pleistocene tectonic development of the Kyrenia Range, N Cyprus: Implications for E Mediterranean Tethyan palaeogeography

Three published alternative tectonic models of the Permian-Pleistocene development of the Kyrenia Range, N Cyprus are tested, supported by new field, geochemical and micropalaeontological evidence: 1. The Kyrenia Range represents the northern continental margin of the S Neotethys, close to its present relative position. The range initiated as a Permian-Cretaceous rift/passive margin, switching to a N-facing active margin during Late Cretaceous-Neogene; 2. The Range was located along the N African continental margin until the Neogene when northward subduction transferred it to the southern margin of the Eurasian plate; 3. The Range is a far-travelled allochthon that was emplaced to near its present position, probably during the Eocene.In the light of regional comparisons, especially with southern Turkey, the combined evidence mainly supports tectonic model 1. Sedimentary and palaeontological data show that the restored stratigraphy of the Kyrenia Range indicates Late Permian initial rifting and Early-Middle Triassic advanced rifting, followed by Jurassic-Early Cretaceous passive margin subsidence. Small exposures of ophiolite-related melange located between the Mesozoic carbonate platform and the overlying latest Cretaceous-Palaeogene deep-water volcanic-sedimentary succession include evidence of HP/LT metamorphism, pointing to Late Cretaceous subduction. MORB/boninites, diabase-gabbro and extensive harzburgitic serpentinite originated as a SSZ ophiolite, together with a possible high-grade metamorphic sole (garnet amphibolite) and an accretionary prism (E-MORB/OIB; metachert). Microfossil evidence indicates exhumation of the melange and the underlying platform prior to Late Maastrichtian. A mass-transport complex formed within a compression-related foredeep during the Middle Eocene. Associated southward thrusting and folding culminated in emergence and subaerial erosion, generating a major unconformity, that was followed by subaerial and then marine deltaic deposits (Late Eocene-Oligocene). Following major Late Miocene southward thrusting, uplift of the Kyrenia Range took place during the Pleistocene, related to collision of the leading edge of the North African plate (Eratosthenes Seamount) with the Eurasian (Anatolian) plate

Late Palaeozoic extensional volcanism along the northern margin of Gondwana in southern Turkey: implications for Palaeotethyan development

The Late Palaeozoic-Early Mesozoic Tethyan development of the Eastern Mediterranean region remains debatable, especially in Turkey, where alternative northward and southward subduction hypotheses are proposed. Relevant to this debate, new whole-rock geochemical data are provided here for early Carboniferous (Late Tournaisian-Late Visean; c. 340-350 Ma) tuffaceous sedimentary rocks within the cataloturan thrust sheet (Aladag nappe), eastern Taurides. The tuffs accumulated from evolved alkaline volcanism, variably mixed with terrigenous and radiolarian-rich sediments. In addition, Late Palaeozoic meta-volcanic rocks, c. 150 km farther NE, within the Binboga (= Malatya) metamorphics (a low-grade high-pressure unit), are indicative of a within-plate setting. An impersistent geochemical subduction signature in these volcanics may represent an inherited, rather than contemporaneous, subduction influence, mainly because of the absence of a continental margin arc or of arc-derived tuff. Both the Binboga metamorphics and the cataloturan thrust sheet (Aladag nappe) restore generally to the north of the relatively autochthonous Tauride carbonate platform (Geyik Dag), within the carbonate platform bordering north-Gondwana. The cataloturan thrust sheet is interpreted, specifically, as a c. E-W, deep-water, volcanically active rift that progressively infilled. Regional geological evidence suggests that melange units (Konya Complex, Afyon zone), Teke Dere unit, Lycian nappes), and Chios-Karaburun melange, E Aegean) accreted to the north-Gondwana continental margin during the late Carboniferous; this was coupled with localised calc-alkaline granitic magmatism (Afyon zone of Anatolide crustal block). We propose an interpretation in which Late Devonian-Carboniferous alkaline intra-plate volcanism relates to extension/rifting along the north-Gondwana margin. In contrast, the melange accretion and granitic magmatism could relate to short-lived late Carboniferous southward subduction that accompanied the diachronous closure of Palaeotethys

Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and

Genetic relationship between some species of Zea mays and Sorghum was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein and random amplification of polymorphic DNA (RAPD-PCR) markers. According to SDS-PAGE analysis, 78 band were identified across the studied species. The number of bands varies from 17 bands in sample number 5 to 6 in sample number 6. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Zea mays and Sorghum species than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Zea mays spp mays and Zea mays spp Mexicana. Further support comes from the molecular data of RAPD, which indicate that close relationship between Sorghum valgare and Sorghum bicolor.Keywords: Zea mays, Sorgum volgare pres, Punciu milia ceaum L. protein, random amplification of polymorphic DNA.African Journal of Biotechnology Vol. 12(27), pp. 4269-427

The elicitation of key performance indicators of e-government providers: A bottom-up approach

Copyright @ 2013 EMCIS.Delivering an adequate e-Government service (e-service) is becoming more of a necessity in today's digital world. In order to improve e-services and increase the engagement of both users' and providers' side, studies on the performance evaluation of such provided e-services are taking places. However a clear identification of the key performance indicators from the e-Government providers’ side is not well explored. This shortcoming hampers the conduct of a holistic evaluation of an e-service provision from the perspective of its stakeholders in order to improve e-services as well as to increase e-services take-ups. In this paper, a systematic process to identify indicators is implemented based on a bottom-up approach. The process used three focus-group meetings with providers, users, and academics in Qatar, Lebanon and UK to collect, identify and validate key indicators from the perspective of e-services’ providers. The approach resulted in the identification of five factors levels (service, technology, employees, policy and management and social responsibilities) with fifteen sub-categories of SMART variables. Hence, leading to the development of a new model, STEPS, that can fully explain and predict e-government success from the providers’ point of view. It will work as a strategic management tool to align various stakeholders on common goal and values based on evidence based evaluation of e-services using smart measurable indicators for the improvement of an e-service at the engagement level in the field of e-government. In addition, other fields can benefit from the outcome of this work, such as logistics service providers, who make their services available across new and existing relationships between the Internet commerce firms, their customers, and their vendors

An Electronically Reconfigurable Patch Antenna Design for Polarization Diversity with Fixed Resonant Frequency

In this paper, an electronically polarization reconfigurable circular patch antenna with fixed resonant frequency operating at Wireless Local Area Network (WLAN) frequency band (2.4-2.48 GHz) is presented. The structure of the proposed design consists of a circular patch as a radiating element fed by coaxial probe, cooperated with four equal-length slits etched on the edge along x-axis and y-axis. A total of four switches was used and embedded across the slits at specific locations, thus controlled the length of the slits. By activating and deactivating the switches (ON and OFF) across the slits, the current on the patch is changed, thus modifying the electric field and polarization of the antenna. Consequently, the polarization excited by the proposed antenna can be switched into three types, either linear polarization, left-hand circular polarization or right-hand circular polarization. This paper proposes a simple approach that able to switch the polarizations and excited at the same operating frequency. Simulated and measured results of ideal case (using copper strip switches) and real case (using PIN diode switches) are compared and presented to demonstrate the performance of the antenna

The condensin complexes play distinct roles to ensure normal chromosome morphogenesis during meiotic division in Arabidopsis

Meiosis is a specialized cell division essential for sexual reproduction. During meiosis the chromosomes are highly organized, and correct chromosome architecture is required for faithful segregation of chromosomes at anaphase I and II. Condensin is involved in chromosome organization during meiotic and mitotic cell divisions. Three condensin subunits, AtSMC4 and the condensin I and II specific subunits AtCAP-D2 and AtCAP-D3, respectively, have been studied for their role in meiosis. This has revealed that both the condensin I and condensin II complexes are required to maintain normal structural integrity of the meiotic chromosomes during the two nuclear divisions. Their roles appear functionally distinct in that condensin I is required to maintain normal compaction of the centromeric repeats and 45S rDNA, whereas loss of condensin II was associated with extensive interchromosome connections at metaphase I. Depletion of condensin is also associated with a slight reduction in crossover formation, suggesting a role during meiotic prophase I

Nonlinear and linear timescales near kinetic scales in solar wind turbulence

The application of linear kinetic treatments to plasma waves, damping, and instability requires favorable inequalities between the associated linear timescales and timescales for nonlinear (e.g., turbulence) evolution. In the solar wind these two types of timescales may be directly compared using standard Kolmogorov-style analysis and observational data. The estimated local (in scale) nonlinear magnetohydrodynamic cascade times, evaluated as relevant kinetic scales are approached, remain slower than the cyclotron period, but comparable to or faster than the typical timescales of instabilities, anisotropic waves, and wave damping. The variation with length scale of the turbulence timescales is supported by observations and simulations. On this basis the use of linear theory—which assumes constant parameters to calculate the associated kinetic rates—may be questioned. It is suggested that the product of proton gyrofrequency and nonlinear time at the ion gyroscales provides a simple measure of turbulence influence on proton kinetic behavior

Evaluation of Biological Activities of Extracts and Chemical Constituents of Mimusops elengi

Purpose: To isolate some compounds from the leaves and bark of Mimusops elengi, and examine them for their antibacterial and anti-inflammatory properties.Experimental: The compounds were isolated from the leaf and bark chloroform extracts using column chromatography, and characterized using physical and spectroscopic methods. The isolated compounds and their respective extracts were tested for antibacterial activity by micro-dilution antibacterial assay, and for anti-inflammatory activity by cyclooxygenase inhibitory assay.Results: of the compounds isolated include spinasterol (1), ursolic acid (2) and 3β, 6β, 19α, 23- tetrahydroxyurs-12-en-28-oic acid (3) from the leaves; and taraxerol (4) and spinasterol β-Dglucopyranoside (5) from the bark. A majority of the samples showed good activity against Staphylococcus aureus (9.7 ─ 78.0 μg/mL), while moderate activity was observed against Gramnegative bacteria (78.0 ─ 156 μg/mL). Strong COX inhibition was observed for the leaf extract, and (1); selective COX-2 inhibition for (2) and (3); and selective COX-1 inhibition for bark extract, (4) and (5).Conclusion: This is the first report describing the anti-inflammatory potential of M. elengi on the basis of its isolated constituents. The results of this study support the traditional use of the plant as antibacterial and anti-inflammatory remedy.Keywords: Mimusops elengi, Sapotaceae, Steroids; Triterpenoids, Antibacterial, Antiinflammatory