Increased Oral Detection, but Decreased Intestinal Signaling for Fats in Mice Lacking Gut Microbiota

Germ-free (GF) mice lacking intestinal microbiota are significantly leaner than normal (NORM) control mice despite consuming more calories. The contribution of microbiota on the recognition and intake of fats is not known. Thus, we investigated the preference for, and acceptance of, fat emulsions in GF and NORM mice, and associated changes in lingual and intestinal fatty acid receptors, intestinal peptide content, and plasma levels of gut peptides. GF and NORM C57Bl/6J mice were given 48-h two-bottle access to water and increasing concentrations of intralipid emulsions. Gene expression of the lingual fatty acid translocase CD36 and protein expression of intestinal satiety peptides and fatty-acid receptors from isolated intestinal epithelial cells were determined. Differences in intestinal enteroendocrine cells along the length of the GI tract were quantified. Circulating plasma satiety peptides reflecting adiposity and biochemical parameters of fat metabolism were also examined. GF mice had an increased preference and intake of intralipid relative to NORM mice. This was associated with increased lingual CD36 (P<0.05) and decreased intestinal expression of fatty acid receptors GPR40 (P<0.0001), GPR41 (P<0.0001), GPR43 (P<0.05), and GPR120 (P<0.0001) and satiety peptides CCK (P<0.0001), PYY (P<0.001), and GLP-1 (P<0.001). GF mice had fewer enteroendocrine cells in the ileum (P<0.05), and more in the colon (P<0.05), relative to NORM controls. Finally, GF mice had lower levels of circulating leptin and ghrelin (P<0.001), and altered plasma lipid metabolic markers indicative of energy deficits. Increased preference and caloric intake from fats in GF mice are associated with increased oral receptors for fats coupled with broad and marked decreases in expression of intestinal satiety peptides and fatty-acid receptors

Utilisation de pellicules de colza dans l'alimentation du lapin en croissance. Effets sur la qualite des carcasses.

National audienc

Utilisation de pellicules de colza dans l'alimentation du lapin en croissance. II. Effet sur la qualite des carcasses.

National audienc

Utilisation de pellicules de colza dans l'alimentation du lapin en croissance. II. - Effet sur la qualité des carcasses

National audienc

Antioxidant effect of cirsimarin, a flavonoid extracted from microtea debilis

International audienceThe free radical scavenging activity of cirsimarin was investigated and compared to flavonoids quercetin and genistein. In vitro, cirsimarin induced a dose-dependent inhibition of lipid peroxidation indicating a moderate antioxidant activity (IC(50) = 370 mu M) when compared with genistein (IC(50) = 170 mu M) and quercetin (IC(50) = 1.4 mu M). Adipose tissue has been recently identified as a major site of reactive oxygen species production, the effect of cirsimarin was tested on basal H(2)O(2) production rate of small pieces of rat white adipose tissue. Cirsimarin (15 mu M) induced a 36 % reduction of H(2)O(2) production by adipose tissue. At that concentration it did not scavenge H(2)O(2). These results suggest that cirsimarin exerts a direct action on hydrogen peroxide production by adipose tissue cells