133 research outputs found
On the formation and decay of a molecular ultracold plasma
Double-resonant photoexcitation of nitric oxide in a molecular beam creates a
dense ensemble of Rydberg states, which evolves to form a plasma of
free electrons trapped in the potential well of an NO spacecharge. The
plasma travels at the velocity of the molecular beam, and, on passing through a
grounded grid, yields an electron time-of-flight signal that gauges the plasma
size and quantity of trapped electrons. This plasma expands at a rate that fits
with an electron temperature as low as 5 K, colder that typically observed for
atomic ultracold plasmas. The recombination of molecular NO cations with
electrons forms neutral molecules excited by more than twice the energy of the
NO chemical bond, and the question arises whether neutral fragmentation plays a
role in shaping the redistribution of energy and particle density that directs
the short-time evolution from Rydberg gas to plasma. To explore this question,
we adapt a coupled rate-equations model established for atomic ultracold
plasmas to describe the energy-grained avalanche of electron-Rydberg and
electron-ion collisions in our system. Adding channels of Rydberg
predissociation and two-body, electron- cation dissociative recombination to
the atomic formalism, we investigate the kinetics by which this relaxation
distributes particle density and energy over Rydberg states, free electrons and
neutral fragments. The results of this investigation suggest some mechanisms by
which molecular fragmentation channels can affect the state of the plasma
Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung
10.1371/journal.pone.0024019PLoS ONE68
The Infection and Impact of Azorhizobium Caulinodans ORS571 on Wheat (Triticum Aestivum L.)
Based on our previous study, cereal crop wheat (Triticum aestivum L.) could be infected by rhizobia Azorhizobium caulinodans ORS571, and form para-nodules with the induction of 2.4-dichlorophenoxyacetic acid, a common plant growth regulator. To enhance this infection and the potential agricultural application, we compared six different infection methods (Direct seed dip; Seed germination dip; Pruned-root dip; Foliar spray; Circum-soil dip; Seed dip and circum-soil dip) for achieving the high efficient infection of A. caulinodans into wheat plants by employing a green fluorescent protein (gfp)-labeled Azorhizobium caulinodans strain ORS571. With proper methods, copious rhizobia could enter the interior and promote the growth of wheat to the hilt. Circum-soil dip was proved to be the most efficient method, seed germination dip and pruned-root dip is the last recommended to infect wheat, seed germination dip and seed dip and circum-soil dip showed better effects on plant growth, pruned-root dip did not show too much effect on plant growth. This study laid the foundation for understanding the interaction between rhizobia and cereal crops and the growth-promoting function of rhizobia
From Mendel’s discovery on pea to today’s plant genetics and breeding
In 2015, we celebrated the 150th anniversary of the presentation of the seminal work of Gregor Johann Mendel. While Darwin’s theory of evolution was based on differential survival and differential reproductive success, Mendel’s theory of heredity relies on equality and stability throughout all stages of the life cycle. Darwin’s concepts were continuous variation and “soft” heredity; Mendel espoused discontinuous variation and “hard” heredity. Thus, the combination of Mendelian genetics with Darwin’s theory of natural selection was the process that resulted in the modern synthesis of evolutionary biology. Although biology, genetics, and genomics have been revolutionized in recent years, modern genetics will forever rely on simple principles founded on pea breeding using seven single gene characters. Purposeful use of mutants to study gene function is one of the essential tools of modern genetics. Today, over 100 plant species genomes have been sequenced. Mapping populations and their use in segregation of molecular markers and marker–trait association to map and isolate genes, were developed on the basis of Mendel's work. Genome-wide or genomic selection is a recent approach for the development of improved breeding lines. The analysis of complex traits has been enhanced by high-throughput phenotyping and developments in statistical and modeling methods for the analysis of phenotypic data. Introgression of novel alleles from landraces and wild relatives widens genetic diversity and improves traits; transgenic methodologies allow for the introduction of novel genes from diverse sources, and gene editing approaches offer possibilities to manipulate gene in a precise manner
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