Isolation of Bacterial Ribosomes with Monolith Chromatography

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology

/STRU^NI RAD 616.348/.351-006.04-089 (497.12)"1991/2000" Treatment results of colorectal cancer – 10- years series of UMC Ljubljana (1991 – 2000)

In Slovenia the incidence of colorectal cancer is growing rapidly. In 1998 1022 new cases were registered. Our study compares results of two groups of patients with colorectal cancer. Patients and methods. In the period from 1.1.1991 to 31.12.2000 1478 patients with a colorectal carcinoma underwent potentially curative resection. We divided them in two groups, one operated in the first 5-years and second in later 5-years period. 5-years survival was estimated with Kaplan-Meier statistical analysis. Patients who died within 30 days after the operation were censored. Differences in survival curves between both groups were assessed by the log rank test. Results. We resected 1478 /1599 (92,4%) patients. There was 913 (61,7%) patients resected with colon cancer and 528 (35,8%) with rectal cancer and 37 (2,5%) with sinhronius tumors. R0 resection was performed in 1174 (79,4%) patients, R1 in 29 (2,0%), and R2 in 273 (18,5%) patients. Postoperative mortality rate in resected patients was 5,48 % (81/1478), in the group with paliative operations was 17,35 % (21/121). Overall five-years survival rate was 54,9 % (56,18% for colon cancer and 52,4 % for rectal cancer Fiveyears survival rate for the patients with radical resection (R0) was 66,54 % for colon cancer and 59,47 % for rectal cancer. Conclusion. 5-years survival for R0-resected patients with colon cancer was in the last period from 1996 to 2000 statistically significantly better compared with the period from 1991 to 1995 (76 % vs 60%) in stage I (p=0,04048) and in stage III (p=0,01842). 5-years survival for R0-resected patients with rectal cancer was significantly better in the same period (63 % vs 55%) (p = 0,03627) in stage III (p=0,01663)

Synthesis and field emission characteristics of W5O14nanowires film

The W5O14 nanowires are metallic oxides with specific resistivity of 27 mu Omega cm and diameters bellow 100 nm. The field emission characteristics of the films composed of these nanowires have been investigated. The emitting current densities up to 6.4 mA/cm(2) have been obtained at very low electric field of about 3 V/mu m. The samples were allowed to emit for more than 100 h without showing significant decays of the emitting current and without substantial current oscillations. These characteristics make these nanowires very promising for the realization of large area field emitting cathodes. (C) 2017 Elsevier B.V. All rights reserved

Secure and interoperable communication infrastructures for PPDR organisations: invited paper

The growing number of events affecting public safety and security (PS&S) on a regional scale with potential to grow up to large scale cross border disasters puts an increased pressure on agencies and organisation responsible for PS&S. In order to respond timely and in an adequate manner to such events, Public Protection and Disaster Relief (PPDR) organisations need to cooperate, align their procedures and activities, share the needed information and be interoperable. Existing PPDR/PMR technologies such as TETRA, TETRAPOL or P25, do not currently provide broadband capability nor is expected such technologies to be upgraded in the future. This presents a major limitation in supporting new services and information flows. Furthermore, there is no known standard that addresses interoperability of these technologies. In this contribution the design of a next generation communication infrastructure for PPDR organisations which fulfills the requirements of secure and seamless end-to-end communication and interoperable information exchange within the deployed communication networks is presented. Based on Enterprise Architecture of PPDR organisations, a next generation PPDR network that is backward compatible with legacy communication technologies is designed and implemented, capable of providing security, privacy, seamless mobility, QoS and reliability support for mission-critical Private Mobile Radio (PMR) voice and broadband data services. The designed solution provides a robust, reliable, and secure mobile broadband communications system for a wide variety of PMR applications and services on PPDR broadband networks, including the ability of inter-system, interagency and cross-border operations with emphasis on interoperability between users in PMR and LTE