Nata organisms: an overview on the fermentative microbial ecosystem

Publicado em "Abstracts of papers - American Chemical Society", vol. 245The Acetobacter and Gluconacetobacter genus (both from the Acetobacteraceae family) are the most notable acetic acid producers, their intermediate metabolites being exploited biotechnologicaly for the production of vinegar, Kombucha, cocoa and nata de coco. Extensive efforts are being made to better understand the dynamic interplay of microbial populations during fermentation processes, with ample literature existing on virtually every food product currently being consumed. In the case of nata de coco, Gluconacetobacter strains have been found to play a key role in cellulose production. Despite abundant literature with isolated cellulose−producing strains, little work has been done in analysing population dynamics of the microbial communities. This presentation will address the microbial interplay in the production of nata de coco, with an overview of the taxonomy of the major acetic acid strains involved. An overview on the efforts and potential implications of upgrading nata de coco production through biotechnology will also be addressed

Bacterial cellulose from lab to market

Book of Abstracts of CEB Annual Meeting 2017Bacterial nanocellulose (BNC) is a nanofibrilar exopolysaccharide synthesized by certain Gram-negative, obligate aerobic, acetic acid bacteria, the Komagataeibacter genus being the most important due to the high cellulose yield obtained. The unique properties of this biopolymer have supported a wide range of potential applications, in human and veterinary medicine, odonthology, pharmaceutical industry, acoustic and filter membranes, biotechnological devices and in the food and paper industry. The largescale production of BNC, through advanced biotechnology has eluded many researches. Historical attempts but on low volume and high-value (mostly for biomedical applications) production can be traced back to the 90s. This presentation will show the main work with BNC by the Funcarb group. Examples of these studies will include the use of BNC in biomedical and food applications. Finally, an overview on the main efforts towards the production of BNC at large scale and potential markets will also be presented.info:eu-repo/semantics/publishedVersio

Bacterial NanoCellulose: what future?

Acetic acid bacteria (AAB) have been used in various fermentation processes. Of several ABB, the bacterial nanocellulose (BNC) producers, notably Komagataeibacter xylinus, appears as an interesting species, in large part because of their ability in the secretion of cellulose as nano/microfibrils. In fact, BNC is characterized by a native nanofibrillar structure, which may outperform the currently used celluloses in the food industry as a promising novel hydrocolloid additive. During the last couple of years, a number of companies worldwide have introduced some BNC-based products to the market. The main aim of this editorial is to underline the BNC potentials.info:eu-repo/semantics/publishedVersio

A spin-offs journey into achieving marketable products from bacterial cellulose

[Excerpt] Academic spin-offs, technological ventures born inside Universities, have increasingly strengthen the connections between the scholarship and the economy, by fostering the role of technology transfer and knowledge commercialization. This presentation will outline the major steps in taking an idea or a technology to market, growing the venture and aiming at securing a successful exit. Also, it will present BCTechnologies (Bacterial Cellulose Technologies), a spin-off from the University of Minho (Portugal). (...

Bacterial cellulose: from biotechnology to bio-economy

Bacterial cellulose (BC) is a nanofibrillar exopolysaccharide synthesized by certain Gramnegative, obligate aerobic, acetic acid bacteria, the Komagataeibacter genus being the most important due to the high cellulose yield obtained. The unique properties of this biopolymer have supported a wide range of potential applications, in human and veterinary medicine, odontology, pharmaceutical industry, acoustic and filter membranes, biotechnological devices and in the food and paper industry. The large-scale production of BC, through advanced biotechnology has eluded many researches. Historical attempts but on low volume and highvalue (mostly for biomedical applications) production can be traced back to the 90s. This presentation will overview the potential uses of BC in several applications. Also, it will present Satisfibre, S.A., a spin-off from the University of Minho (Portugal). Through R&D activities, networking & partnering with industry, Satisfibre aims to bring new and improved solutions, based on the use BC, to the food sector, biomedical, composites, pulp & paper and textile industries. Examples of successful product development and industry networking will be shown. Finally, an overview on the main efforts towards the production of BC at large scale and potential markets will also be presented.info:eu-repo/semantics/publishedVersio

Properties and recycling of covalently immobilized glycanases used for aqueous enzyme assisted Rosa mosqueta oil extraction

2nd International Conference on Protein Stabilisation - From Molecular Interpretation to Bio-Industrial Applicationsinfo:eu-repo/semantics/publishedVersio

Effect of cellulase adsorption on the surface and interfacial properties of cellulose

The surface properties of several purified cellulose (Sigmacell 101, Sigmacell 20, Avicel pH 101, andWhatman CF 11) were characterised, before and after cellulase adsorption. The following techniques were used: thin-layer wicking (except for the cellulose Whatman), thermogravimetry, and differential scanning calorimetry (for all of the above celluloses). The results obtained from the calorimetric assays were consistent with those obtained from thinlayer wicking – Sigmacell 101, a more amorphous cellulose, was the least hydrophobic of the analysed celluloses, and had the highest specific heat of dehydration. The other celluloses showed less affinity for water molecules, as assessed by the two independent techniques. The adsorption of protein did not affect the amount of water adsorbed by Sigmacell 101. However, this water was more strongly adsorbed, since it had a higher specific heat of dehydration. The more crystalline celluloses adsorbed a greater amount of water, which was also more strongly bound after the treatment with cellulases. This effect was more significant for Whatman CF-11. Also, the more crystalline celluloses became slightly hydrophilic, following protein adsorption, as assessed by thin-layer wicking. However, this technique is not reliable when used with cellulase treated celluloses.Junta Nacional de Investigação Científica e Tecnológica (JNICT) - Project INTERREGII/BM/03/REGII/3/97

A dry bacterial cellulose-carboxymethyl cellulose formulation as stabilizer for pickering oil-in-water emulsions

Hydrocolloidal microcrystalline cellulose (MCC) from plant sources, is already widely used in industry to regulate the stability, texture, rheology and organoleptic properties of many food and cosmetic formulations. Bacterial cellulose (BC) is produced biotechnologically by different microorganisms, but most efficiently by acetic acid bacteria from the genera Komagataeibacter. This biomaterial is a prominent alternative to the already marketed celluloses, being more pure, crystalline, and having nanoscale fibres with high aspect ratio which account for excellent mechanical properties. BC has already been used in its hydrated form for the stabilization of oil-in-water (o/w) Pickering emulsions (particle-stabilized systems, as an alternative for the conventional surfactant-stabilized). For the sake of storage, economy and practicality, additives for industries are preferentially provided in a dry or powder form. Co-drying cellulose fibres or crystals with water soluble polysaccharides helps maintaining the rheologic and structuring properties after rehydration. Dry powdered, rehydratable bacterial cellulose (BC) formulations are reported, being produced by different grinding, drying and dispersing methods which were studied in terms of the impact in the final products properties. The main objective of this study was to assess the stabilizing properties of BC in Pickering o/w emulsions. For this, an equimassic formulation of BC and 90 kDa carboxymethyl cellulose (BC:CMC) was prepared and spray dried. Isohexadecane-in-water emulsions (10:90) were prepared in the presence of 0.10%, 0.25% and 0.50% of the BC:CMC formulation. Visual and microscopic aspect of the emulsions was registered over time. Samples were also visualized in Cryo-SEM. Rheological tests were performed to assess the emulsions viscosity profile, storage and loss moduli. Interfacial tension between the immiscible phases was measured with the Pendant Drop and Du Noüy Ring methods. For benchmarking purposes, the same emulsion preparation and analysis protocol was made with several different commercial cellulosic products and xanthan gum. In short, BC:CMC showed formation of a three-dimensional network and viscosity increasing (thickening) properties, crucial characteristics for emulsion stabilizing formulations. BC has technically superior properties that will allow it to compete with, or even replace, plant celluloses in industry.info:eu-repo/semantics/publishedVersio