El complejo karstico-yesifero subterráneo "Pedro Fernández" (Estremera, provincia de Madrid)

[Resumen] Se han analizado los factores originales de este morfosistema kárstico subterraneo, detallan do los procesos responsables de su génesis, así como la morfolmgía interior. Diferenciando los tipos de galerias y su evolución. Al mismo tiempo, se aportan determinadas consideraciones acerca de la sedimentación interior de la cavidad, su tipología e interpretación de su significado[Resumé] On a analysé les facteurs qui ont originé ce morphosysthéme karstique souterrain, en detallant le procesus responsable de leur genére, ainsi que leur morphilogie intérieure. En distinguant les types de galeries et leur évolution. Eu méme temps, on apporte certaines considerations données á propos de la sédimentation, sa typologie et interprétation de sa signification

Retrieving leaf area index from multi-angular airborne data

This work is aimed to demonstrate the feasibility of a methodology for retrieving bio-geophysical variables whilst at the same time fully accounting for additional information on directional anisotropy. A model-based approach has been developed to deconvolve the angular reflectance into single landcovers reflectances, attempting to solve the inconsistencies of 1D models and linear mixture approaches. The model combines the geometric optics of large scale canopy structure with principles of radiative transfer for volume scattering within individual crowns. The reliability of the model approach to retrieve LAI has been demonstrated using data from DAISEX- 99 campaign at Barrax, Spain. Airborne data include POLDER and HyMap data in which various field plots were observed under varying viewing/illumination angles. Nearly simultaneously, a comprehensive field data set was acquired on specific crop plots. The inversions provided accurate LAI values, revealing the model potential to combine spectral and directional information to increase the likely accuracy of the retrievals. In addition, the sensitivity of retrievals with the angular and spectral subset of observations was analysed, showing a high consistency between results. This study has contributed to assess the uncertainties with products derived from satellite data like SEVIRI/MSG

Origin of slow magnetic relaxation in Kramers ions with non-uniaxial anisotropy

Transition metal ions with long-lived spin states represent minimum size magnetic bits. Magnetic memory has often been associated with the combination of high spin and strong uniaxial magnetic anisotropy. Yet, slow magnetic relaxation has also been observed in some Kramers ions with dominant easy-plane magnetic anisotropy, albeit only under an external magnetic field. Here we study the spin dynamics of cobalt(II) ions in a model molecular complex. We show, by means of quantitative first-principles calculations, that the slow relaxation in this and other similar systems is a general consequence of time-reversal symmetry that hinders direct spin–phonon processes regardless of the sign of the magnetic anisotropy. Its magnetic field dependence is a subtle manifestation of electronuclear spin entanglement, which opens relaxation channels that would otherwise be forbidden but, at the same time, masks the relaxation phenomenon at zero field. These results provide a promising strategy to synthesize atom-size magnetic memories

Pt(II) coordination complexes as visible light photocatalysts for the oxidation of sulfides using batch and flow processes

A new catalytic system for the photooxidation of sulfides based on Pt(ii) complexes is presented. The catalyst is capable of oxidizing a large number of sulfides containing aryl, alkyl, allyl, benzyl, as well as more complex structures such as heterocycles and methionine amino acid, with complete chemoselectivity. In addition, the first sulfur oxidation in a continuous flow process has been developedJ. A. would also like to thank the MICINN for their ‘Ramón y Cajal’ contract and the European Research Council (ERC-CG, contract number 647550

Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum

Filamentous fungi encode distinct antifungal proteins (AFPs) that offer great potential to develop new antifungals. Fungi are considered immune to their own AFPs as occurs in Penicillium chrysogenum, the producer of the well-known PAF. The Penicillium digitatum genome encodes only one afp gene (afpB), and the corresponding protein (AfpB) belongs to the class B phylogenetic cluster. Previous attempts to detect AfpB were not successful. In this work, immunodetection confirmed the absence of AfpB accumulation in wild type and previous recombinant constitutive P. digitatum strains. Biotechnological production and secretion of AfpB were achieved in P. digitatum with the use of a P. chrysogenum-based expression cassette and in the yeast Pichia pastoris with the α-factor signal peptide. Both strategies allowed proper protein folding, efficient production and single-step purification of AfpB from culture supernatants. AfpB showed antifungal activity higher than the P. chrysogenum PAF against the majority of the fungi tested, especially against Penicillium species and including P. digitatum, which was highly sensitive to the self-AfpB. Spectroscopic data suggest that native folding is not required for activity. AfpB also showed notable ability to withstand protease and thermal degradation and no haemolytic activity, making AfpB a promising candidate for the control of pathogenic fungi

Intraretinal Transplantation for Rod-Cell Replacement in Light-Damaged Retinas

Blindness from retinal disease is often the consequence of extensive damage to the photoreceptor cell population, while other cell types which form the neural retina are relatively spared. In this setting, transplantation of photoreceptor cells could offer hope for the restoration of some degree of visual function. We testd the feasibility of this approach by transplanting immature retinal cells into the eyes of adult rats affected by late stage phototoxic retinopathy, which are almost totally devoid of photoreceptor cells

A Post Hoc Analysis of KidneyIntelX and Cardiorenal Outcomes in Diabetic Kidney Disease

KidneyIntelX, a bioprognostic test for assessing risk of CKD progression, risk stratified individuals for kidney, heart failure, and death outcomes in the Canagliflozin Cardiovascular Assessment Study.Individuals scored as high risk seemed to derive more of benefit from treatment with canagliflozin versus placebo.These findings may serve to increase adoption of underutilized therapies for cardiorenal risk reduction in patients with diabetic kidney disease

Utilização de esterco como medida auxiliar no controle da murcha-bacteriana em genótipos de batata.

Avaliou-se o efeito da aplicação de estercos compostados sobre a incidência de murcha-bacteriana (MB) nas cultivares Agata, Monalisa e Bintje, em campo naturalmente infestado com Ralstonia solanacearum. Utilizou-se o delineamento experimental de parcelas sub-subdivididas, desenho fatorial 3 x 2 x 4, referente a três cultivares, dois tipos de esterco ? de gado e de galinha ?, quatro doses (0, 10, 20, e 30 Mg . ha-1 para o esterco de galinha e 0, 15, 30 e 45 Mg . ha-1 para o esterco de gado) e seis repetições. De forma a verificar se o efeito da aplicação do esterco na incidência de MB estava relacionado à disponibilização de enxofre e nitrogênio, aplicou-se 300 kg ha-1 de sulfato de amônio nas parcelas referentes a um dos tratamentos-testemunha sem esterco. Essa comparação foi considerada como experimento adicional e não como uma subparcela do experimento central. Avaliou-se a incidência da doença, a produtividade total e a produtividade comercial. Os resultados evidenciaram aumento da produção associado à redução na incidência de MB, observada principalmente com aplicação de 10 Mg . ha-1 de esterco de galinha. A redução verificada ocorreu de forma mais acentuada nas cultivares Bintje e Monalisa em comparação com a cultivar Achat, que, independentemente da aplicação de esterco, apresentou menor incidência de MB. A aplicação em cobertura de sulfato de amônio também reduziu a incidência de murcha bacteriana em ?Bintje? e ?Monalisa? e isso sugere que o efeito supressivo a essa doença, obtido com aplicação do esterco de galinha, seja devido, pelo menos em parte, ao fornecimento de enxofre e nitrogênio ao sistema.bitstream/item/177634/1/Rev-Latinoamericana-de-la-papa-v-17-n1-p152-169-2012.pd

Clinical utility of KidneyIntelX in early stages of diabetic kidney disease in the CANVAS trial

Introduction: KidneyIntelX is a composite risk score, incorporating biomarkers and clinical variables for predicting progression of diabetic kidney disease (DKD). The utility of this score in the context of sodium glucose co-transporter 2 inhibitors and how changes in the risk score associate with future kidney outcomes are unknown. Methods: We measured soluble tumor necrosis factor receptor (TNFR)-1, soluble TNFR-2, and kidney injury molecule 1 on banked samples from CANagliflozin cardioVascular Assessment Study (CANVAS) trial participants with baseline DKD (estimated glomerular filtration rate [eGFR] 30-59 mL/min/1.73 m2 or urine albumin- to-creatinine ratio [UACR] ≥30 mg/g) and generated KidneyIntelX risk scores at baseline and years 1, 3, and 6. We assessed the association of baseline and changes in Kidney- IntelX with subsequent DKD progression (composite outcome of an eGFR decline of ≥5 mL/min/year [using the 6-week eGFR as the baseline in the canagliflozin group], ≥40% sustained decline in the eGFR, or kidney failure). Results: We included 1,325 CANVAS participants with concurrent DKD and available baseline plasma samples (mean eGFR 65 mL/min/1.73 m2 and median UACR 56 mg/g). During a mean follow-up of 5.6 years, 131 participants (9.9%) experienced the composite kidney outcome. Using risk cutoffs from prior validation studies, KidneyIntelX stratified patients to low- (42%), intermediate- (44%), and high-risk (15%) strata with cumulative incidence for the outcome of 3%, 11%, and 26% (risk ratio 8.4; 95% confidence interval [CI]: 5.0, 14.2) for the high-risk versus low-risk groups. The differences in eGFR slopes for canagliflozin versus placebo were 0.66, 1.52, and 2.16 mL/min/1.73 m2 in low, intermediate, and high KidneyIntelX risk strata, respectively. KidneyIntelX risk scores declined by 5.4% (95% CI: -6.9, -3.9) in the canagliflozin arm at year 1 versus an increase of 6.3% (95% CI: 3.8, 8.7) in the placebo arm (p < 0.001). Changes in the KidneyIntelX score at year 1 were associated with future risk of the composite outcome (odds ratio per 10 unit decrease 0.80; 95% CI: 0.77, 0.83; p < 0.001) after accounting for the treatment arm, without evidence of effect modification by the baseline KidneyIntelX risk stratum or by the treatment arm. Conclusions: KidneyIntelX successfully risk-stratified a large multinational external cohort for progression of DKD, and greater numerical differences in the eGFR slope for canagliflozin versus placebo were observed in those with higher baseline KidneyIntelX scores. Canagliflozin treatment reduced KidneyIntelX risk scores over time and changes in the KidneyIntelX score from baseline to 1 year associated with future risk of DKD progression, independent of the baseline risk score and treatment arm