Finding the missing link in single use fermentation line : Evaluation of single use bioreactors for aerobic bacteria

The application of single use technologies for the bioproduction represents a major technology innovation advance. Single use bioreactors for cell culture have been largely implemented for manufacturing, providing significant flexibility, costs reduction and fast implementations. In contrast, few technologies are available for aerobic bacterial fermentation mainly due to limitations for oxygen and heat transfer. Like clinical products derived from bacterial expression system still represent a significant number of bio therapeutics/vaccines, some investigations have been performed in Sanofi Pasteur to evaluate new single use fermenters suitable for aerobic process and matching to clinical manufacturing scale. The 300L HyPerforma SUF (ThermoFisher) has been evaluated with an E.coli strain to produce a recombinant protein and with yeast

Phylogenetic Studies of the Bullous Pemphigoid Antigen-1 Using Human Monoclonal-antibodies

Bullous pemphigoid is an autoimmune skin disorder with production of autoantibodies against bullous pemphigoid antigen 1 (BPAg1) and bullous pemphigoid antigen 2 (BPAg2) which are constitutively expressed in hemdesmosomes. Phylogenetic study of the reactivity of 3 human monoclonal antibodies (HuMabs) specific for BPAg1 was performed using immunohistochemical analysis of skin sections. The serum of the 2 BP patients from which the 3 HuMabs were derived stained the basement membrane zone on skin cryostat section of all species tested including fishes, amphibians, reptiles, birds and mammals. However, of the 3 HuMabs BP1, BP2 and BP3, only BP3 reacted with the skin of reptiles, birds and mammals while Bp1 and Bp2 exclusively bound to mammalian skin. These data provide evidence for the existence of multiple epitopes on BPAg1 able to bind autoantibodies