Extraction, purification and analysis of thermal stability of xylose isomerase

Thermostable enzymes are the enzymes which active even at high temperatures, such enzymes are industrially as well as biochemically very important. Xylose isomerase (EC 5.3.1.5) is one such enzyme with suitable commercial applications. It is heat stable and does not require expensive cofactors such as NAD or ATP for activity. The microorganisms producing this enzyme were isolated from hot water spring near ‘Surat’. The organisms were isolated and purified by using different screening methods. The isolated organisms were then subjected to optimum growth conditions for enzyme production. This enzyme was then assayed for its thermal stability at elevated temperatures by using DNSA

Analysis of protease activity of enzyme isolated from compost soil

Bacteria are very good source of enzymes as compared to animal or plant source and even synthetic enzymes. In the present investigation the protease activity has been analyzed. The source of enzyme i.e. protease producers were isolated from compost soil sample viz. collected from the Wanker farm field, Solapur. The protease producers were isolated, screened and grown on a suitable growth medium to obtain maximum production of enzyme. After production the crude enzyme is purified. The purified enzyme is analysed for its keratinolytic activity by using feathers