Mini-Collagens in Hydra Nematocytes

We have isolated and characterized four collagen-related c-DNA clones (N-COL 1, N-COL 2, N-COL 3, N-COL 4) that are highly expressed in developing nematocytes in hydra. All four c-DNAs as well as their corresponding transcripts are small in size (600-1,000 bp). The deduced amino acid sequences show that they contain a central region consisting of 14 to 16 Gly-X-Y triplets. This region is flanked amino-terminal by a stretch of 14-23 proline residues and carboxy-terminal by a stretch of 6-9 prolines. At the NH2- and COOH-termini are repeated patterns of cysteine residues that are highly conserved between the molecules. A model is proposed which consists of a central stable collagen triple helix of 12-14 nm length from which three 9-22 nm long polyproline II type helices emerge at both ends. Disulfide linkage between cysteine- rich segments in these helices could lead to the formation of oligomeric network structures. Electrophoretic characterization of nematocyst extracts allows resolution of small proline-rich polypeptides that correspond in size to the cloned sequences

Strengthening emotional development and emotion regulation in childhood

The following article deals with emotional development and the development of emotion regulation skills in children during early childhood education, focusing primarily on the importance of the early childhood teacher. Emotion regulation is important for success and wellbeing in further life. It is developed in interaction with parents as attachment figures. Teachers can also be important persons for the child in the context of bonding. This leads to the question of how early childhood teachers can support children learning to regulate their emotions. We analyze with the content analysis, four programs for promoting social and emotional skills that are currently used in Germany. The main question is if the programs include elements that increase teachers’ skills in supporting the children in regulating their emotions. The categories to analyze the programs are derived from theories of teacher-child interaction. In addition to programs for promoting emotional and social development, we will discuss aspects of shaping interaction as essential elements in promoting emotion regulation. The conclusion outlines some key implications for educational practice and the importance of developing professional behavior for qualitative teacher-child interactions

Moses Mendelssohn: Die Doppelexistenz des Philosophen

Wer von uns weiß heute noch, dass dieser "Sokrates der 18. Jahrhunderts", der nach 1728 nur 57 1/3 Jahre lebte, ebenso sehr als Algebraist und Handelsmann anerkannt und hoch geschätzt war? So rundet das Leben dieses körperlich und sozial benachteiligten Juden gleichzeitig ein gewichtiges Kapitel der Geschichte Brandenburgs Preußens. – Und zwar zu einer Zeit, als dies kleine Königtum noch immer an den Folgen des Dreißigjährigen Krieges krankte und dem Bankrott nahe war. Somit kamen viele ökonomische, historische, politische Tatsachen ins Spiel, die an diesem Geschehen beteiligt waren, als der 14jährige aus seiner Vaterstadt Dessau 1743 seinem Lehrer, dem gerade ernannten Oberrabbiner Brandenburgs, ins Ausland, nach Berlin, folgte. Dies zu einer Zeit, wo seit 1740 die Juden im Lande des Philosophenkönigs Friedrich II durch die schmähliche Neuabfassung des Judenreglements unter entwürdigenden Bedingungen existierten. Trotzdem war und wurde dies ein ungewöhnlich günstiger Zeitpunkt in der Geschichte Brandenburgs. Denn die Hauptstadt verfügte, trotz aller physischen und sozialen Nachteile, über ein sehr reges intellektuelles Klima, hauptsächlich dank des Einflusses der Hugenottischen Immigranten

Dynamics of organic matter and bacterial activity in the Fram Strait during summer and autumn

The Arctic Ocean is considerably affected by the consequences of global warming, including more extreme seasonal fluctuations in the physical environment. So far, little is known about seasonality in Arctic marine ecosystems in particular microbial dynamics and cycling of organic matter. The limited characterization can be partially attributed to logistic difficulties of sampling in the Arctic Ocean beyond the summer season. Here, we investigated the distribution and composition of dissolved organic matter (DOM), gel particles and heterotrophic bacterial activity in the Fram Strait during summer and autumn. Our results revealed that phytoplankton biomass influenced the concentration and composition of semi-labile dissolved organic carbon (DOC), which strongly decreased from summer to autumn. The seasonal decrease in bioavailability of DOM appeared to be the dominant control on bacterial abundance and activity, while no temperature effect was determined. Additionally, there were clear differences in transparent exopolymer particles (TEP) and Coomassie Blue stainable particles (CSP) dynamics. The amount of TEP and CSP decreased from summer to autumn, but CSP was relatively enriched in both seasons. Our study therewith indicates clear seasonal differences in the microbial cycling of organic matter in the Fram Strait. Our data may help to establish baseline knowledge about seasonal changes in microbial ecosystem dynamics to better assess the impact of environmental change in the warming Arctic Ocean

Bi-Lateral Changes to Hippocampal Cholesterol Levels During Epileptogenesis and in Chronic Epilepsy Following Focal-Onset Status Epilepticus in Mice

Brain cholesterol homeostasis has been shown to be disrupted in neurodegenerative conditions such as Alzheimer\u27s and Huntington\u27s diseases. Investigations in animal models of seizure-induced brain injury suggest that brain cholesterol levels are altered by prolonged seizures (status epilepticus) and are a feature of the pathophysiology of temporal lobe epilepsy. The present study measured hippocampal sterol levels in a model of unilateral hippocampal injury triggered by focal-onset status epilepticus, and in chronically epileptic mice. Status epilepticus was induced by intra-amygdala microinjection of kainic acid and ipsilateral and contralateral hippocampus analyzed. No significant changes were found for ipsilateral or contralateral hippocampal levels of desmosterol or lathosterol at any time after SE as measured by gas chromatography–mass spectrometry. 24S-hydroxycholesterol and cholesterol levels were unchanged up to 24 h after status epilepticus but were decreased in the ipsilateral hippocampus during early epileptogenesis and in chronically epileptic mice. Levels of cholesterol were also reduced in the contralateral hippocampus during epileptogenesis and in chronic epileptic mice. Treatment of mice with the anti-inflammatory cholesterol synthesis inhibitor lovastatin did not alter seizures during status epilepticus or seizure-induced neuronal death. Thus, changes to hippocampal cholesterol homeostasis predominantly begin during epileptogenesis, occur bi-laterally even when the initial precipitating injury is unilateral, and continue into the chronic epileptic period

The Level of Awareness on the Green ICT Concept and Self Directed Learning among Malaysian Facebook Users

AbstractThe Green information and communication technology is introduced to support the implementation of the green environment. Recent initiatives of promoting green technology and green economy which include “green manufacturing hub “green infrastructure”, low carbon emission, efficient use of resources and a healthy, well- educated populace. For this study, the independent variable is the self directed learning readiness while the dependent variable is the level of awareness on Green ICT. The sample size is seventy seven student adult learners. Random sampling is the sampling method used for this study. The study is to highlight the level of awareness among Malaysian Facebook users

De-repression of myelin-regulating gene expression after status epilepticus in mice lacking the C/EBP homologous protein CHOP.

The C/EBP homologous protein CHOP is normally present at low levels in cells but increases rapidly after insults such as DNA damage or endoplasmatic reticulum stress where it contributes to cellular homeostasis and apoptosis. By forming heterodimers with other transcription factors, CHOP can either act as a dominant-negative regulator of gene expression or to induce the expression of target genes. Recent work demonstrated that seizure-induced hippocampal damage is significantly worse in mice lacking CHOP and these animals go on to develop an aggravated epileptic phenotype. To identify novel CHOP-controlled target genes which potentially influence the epileptic phenotype, we performed a bioinformatics analysis of tissue microarrays from chop-deficient mice after prolonged seizures. GO analysis revealed genes associated with biological membranes were prominent among those in the chop-deficient array dataset and we identified myelin-associated genes to be particularly de-repressed. These data suggest CHOP might act as an inhibitor of myelin-associated processes in the brain and could be targeted to influence axonal regeneration or reorganisation