Conceptualizing Student Practice for the 21st Century: Educational and Ethical Considerations in Modernizing the District of Columbia Student Practice Rules

This article traces the history of the amendment process. It provides a short history of student practice rules and then, using the student practice rule in effect in the District of Columbia prior to the 2014 amendments, describes the various components of those rules that courts and bars across the nation have implemented to assist courts, advance legal education, and preserve advocates’ ethical obligations to clients. It then describes some of the comments to the proposed amendments offered by the District of Columbia Bar and other D.C. lawyers during the public comment period and the modifications to the District of Columbia student practice rule that the District of Columbia Court of Appeals accepted. Finally, it discusses some areas of disagreement that arose during the process and a description of the reasons for those disagreements

Aloysia macrostachya Moldenke

https://thekeep.eiu.edu/herbarium_specimens_byname/18934/thumbnail.jp

Morphological Variation of Rusty Crayfish Orconectes rusticus (Cambaridae) with Gender and Local Scale Spatial Gradients

We tested for the influence of gender, stream, and urbanization on morphological variation in rusty crayfish (Orconectes rusticus) in an east-central Indiana, US watershed. We used geometric morphometrics to characterize shape and tested for differences among and within sites. Males had shallower rostrum, increased head width and length, decreased abdomen and cephalothorax width and length, and increased telson length compared to females. Morphology of males did not vary with stream or along an urban gradient. The morphology of females varied with stream and along an urban gradient. Female shapes from small creek sites were stouter and less fusiform than larger river specimens. Following an urban gradient, females exhibited an increasingly reduced abdominal and telson area and a more fusiform rostrum. Morphological variation is linked with adaptation and subsequent success of aquatic taxa. Disentangling the potential influences on crayfish morphology has implications for improved understanding of ecosystem structure and conservation

The Pure Rotational Spectra of Diatomics and Halogen-Addition Benzene Measured by Microwave and Radio Frequency Spectrometers

Two aluminum spherical mirrors with radii of 203.2 mm and radii of curvature also of 203.2 mm have been used to construct a tunable Fabry-Perót type resonator operational at frequencies as low as 500 MHz. The resonator has been incorporated into a pulsed nozzle, Fourier transform, Balle-Flygare spectrometer. The spectrometer is of use in recording low J transitions of large asymmetric molecules where the spectra are often greatly simplified compared to higher frequency regions. The resonators use is illustrated by recording the rotational spectra of bromobenzene and iodobenzene. In related experiments, using similar equipment, the pure rotational spectra of four isotopomers of SrS and all three naturally occurring isotopomers of the actinide-containing compound thorium monoxide have been recorded between 6 and 26 GHz. The data have been thoroughly analyzed to produce information pertaining to bond lengths and electronic structures

State-space models to describe survival of an endemic species in the Little Tennessee River basin

Endemic species are threatened by invasive species, habitat loss, and climate change. Endemic species are also an important group that maintains biodiversity. Understanding population dynamics of endemic species is needed to maintain or restore their populations. Advancements in models that describe population dynamics of endemic species and species of conservation need has been made possible by the application of novel quantitative methods. One such modeling tool is state-space modeling. These models provide a flexible framework to describe population dynamics using simple mortality models and more complex integrated population models. Here we develop a state-space model to describe survival and population size of the Sicklefin Redhorse (Catostomidae: Moxostoma sp.), a species of conservation concern from two rivers located in North Carolina, USA. This model is structured to combine information across similar rivers and to account for complex interactions of sex, time, variable sampling effort, and river discharge. Survival of Sicklefin Redhorse was found to vary by sex, and annual variability was not consistent across rivers. Discharge was negatively related to capture probability for males. Capture probabilities also differed across sex. Population estimates revealed a large difference between sex where males outnumbered females each year in both rivers. We conclude that electrofishing is not an efficient capture method but when used, should consider discharge. Discharge was not included in the survival model, however, the 3 years with the lowest survival in the Little Tennessee River coincided with the three lowest discharge years in the time series. Future work should investigate the difference in survival between the rivers

Intranasal Ketamine for Acute Pain

Objectives: The aim was to review current evidence regarding the off-label use of intranasal ketamine for acute pain presenting in the setting of the emergency department, and secondary to pediatric limb injuries, renal colic, digital nerve block, and migraines. Results: In all 5 indications reviewed, ketamine demonstrated efficacy in reducing pain. However, when compared with other agents, ketamine did not demonstrate superiority over opioids in pediatric limb injuries or renal colic and was not as efficacious as standard therapy for migraine relief. Ketamine was also associated with a greater incidence of transient adverse reactions, such as dizziness, bitter aftertaste, fatigue, and vomiting than opioid therapies. Discussion: The current body of evidence is insufficient to support the use of intranasal ketamine over other standard therapies for acute pain. However, current evidence can be used when developing dosing strategies, preparing for adverse reactions, and generating hypotheses for future, more robust research

Percussion Convocation

Program listing performers and works performe

Re-localization of Cellular Protein SRp20 during Poliovirus Infection: Bridging a Viral IRES to the Host Cell Translation Apparatus

Poliovirus IRES-mediated translation requires the functions of certain canonical as well as non-canonical factors for the recruitment of ribosomes to the viral RNA. The interaction of cellular proteins PCBP2 and SRp20 in extracts from poliovirus-infected cells has been previously described, and these two proteins were shown to function synergistically in viral translation. To further define the mechanism of ribosome recruitment for the initiation of poliovirus IRES-dependent translation, we focused on the role of the interaction between cellular proteins PCBP2 and SRp20. Work described here demonstrates that SRp20 dramatically re-localizes from the nucleus to the cytoplasm of poliovirus-infected neuroblastoma cells during the course of infection. Importantly, SRp20 partially co-localizes with PCBP2 in the cytoplasm of infected cells, corroborating our previous in vitro interaction data. In addition, the data presented implicate the presence of these two proteins in viral translation initiation complexes. We show that in extracts from poliovirus-infected cells, SRp20 is associated with PCBP2 bound to poliovirus RNA, indicating that this interaction occurs on the viral RNA. Finally, we generated a mutated version of SRp20 lacking the RNA recognition motif (SRp20ΔRRM) and found that this protein is localized similar to the full length SRp20, and also partially co-localizes with PCBP2 during poliovirus infection. Expression of this mutated version of SRp20 results in a ∼100 fold decrease in virus yield for poliovirus when compared to expression of wild type SRp20, possibly via a dominant negative effect. Taken together, these results are consistent with a model in which SRp20 interacts with PCBP2 bound to the viral RNA, and this interaction functions to recruit ribosomes to the viral RNA in a direct or indirect manner, with the participation of additional protein-protein or protein-RNA interactions

Enterovirus 71 3C Protease Cleaves a Novel Target CstF-64 and Inhibits Cellular Polyadenylation

Identification of novel cellular proteins as substrates to viral proteases would provide a new insight into the mechanism of cell–virus interplay. Eight nuclear proteins as potential targets for enterovirus 71 (EV71) 3C protease (3Cpro) cleavages were identified by 2D electrophoresis and MALDI-TOF analysis. Of these proteins, CstF-64, which is a critical factor for 3′ pre-mRNA processing in a cell nucleus, was selected for further study. A time-course study to monitor the expression levels of CstF-64 in EV71-infected cells also revealed that the reduction of CstF-64 during virus infection was correlated with the production of viral 3Cpro. CstF-64 was cleaved in vitro by 3Cpro but neither by mutant 3Cpro (in which the catalytic site was inactivated) nor by another EV71 protease 2Apro. Serial mutagenesis was performed in CstF-64, revealing that the 3Cpro cleavage sites are located at position 251 in the N-terminal P/G-rich domain and at multiple positions close to the C-terminus of CstF-64 (around position 500). An accumulation of unprocessed pre-mRNA and the depression of mature mRNA were observed in EV71-infected cells. An in vitro assay revealed the inhibition of the 3′-end pre-mRNA processing and polyadenylation in 3Cpro-treated nuclear extract, and this impairment was rescued by adding purified recombinant CstF-64 protein. In summing up the above results, we suggest that 3Cpro cleavage inactivates CstF-64 and impairs the host cell polyadenylation in vitro, as well as in virus-infected cells. This finding is, to our knowledge, the first to demonstrate that a picornavirus protein affects the polyadenylation of host mRNA