Consideraciones en torno al problema de la fe y la razón en la obra literaria de Ramon Llull

Backbone mimicry by the formation of closed-loop $C_7,C_{10}$ and $C_{13}$ (mimics of $\gamma-, \beta-$ and $\alpha$-turns) conformations through side chain–main chain hydrogen bonds by polar groups is a frequent observation in protein structures. A data set of 250 non-homologous and high-resolution protein crystal structures was used to analyze these conformations for their characteristic features. Seven out of the nine polar residues (Ser, Thr, Asn, Asp, Gln, Glu and His) have hydrogen bonding groups in their side chains which can participate in such mimicry and as many as 15% of all these polar residues engage in such conformations. The distributions of dihedral angles of these mimics indicate that only certain combinations of the dihedral angles involved aid the formation of these mimics. The observed examples were categorized into various classes based on these combinations, resulting in well defined motifs. Asn and Asp residues show a very high capability to perform such backbone secondary structural mimicry. The most highly mimicked backbone structure is of the$C_{10}$ conformation by the Asx residues. The mimics formed by His, Ser, Thr and Glx residues are also discussed. The role of such conformations in initiating the formation of regular secondary structures during the course of protein folding seems significant

Stranded in isolation: structural role of isolated extended strands in proteins

Reasons for the formation of extended-strands (E-strands) in proteins are often associated with the formation of β -sheets. However E-strands, not part of β-sheets, commonly occur in proteins. This raises questions about the structural role and stability of such isolated E-strands. Using a dataset of 250 largely non-homologous and high-resolution (<2 Å) crystal structures of proteins, we have identified 518 isolated E-strands from 187 proteins. The two most distinguishing features of isolated E-strands from β-strands in β-sheets are the high preponderance of prolyl residues occuring in isolated E-strands and their high exposure to the surroundings. Removal of regions with polyproline conformation from the dataset did not significantly reduce the propensity of prolyl residues to occur in isolated E-strands. Isolated E-strands are often characterized by their main-chain amide and carbonyl groups involved in hydrogen bonding with polar side chains or water. They are often flanked by irregular loop structures and are less well conserved, than β-sheet forming β-strands, among homologous protein structures. It is suggested that isolated β-strands have many characteristics of loop segments but with repetitive (φ,ψ) values falling within the β-region of the Ramachandran map

A randomized, controlled trial of interferon-β-1a (Avonex(®)) in patients with rheumatoid arthritis: a pilot study [ISRCTN03626626]

The objective of this study was to evaluate the safety and possible efficacy of IFN-β-1a for the treatment of patients with rheumatoid arthritis (RA). Twenty-two patients with active RA were enrolled in a phase II randomized, double-blind, placebo-controlled trial of 30 μg IFN-β-1a by weekly self-injection for 24 weeks. The primary outcome of the study was safety. Secondary outcomes included the proportion of patients achieving an American College of Rheumatology (ACR) 20 response at 24 weeks. There were no significant differences in adverse events reported in the two groups. Fewer than 20% of patients in each arm of the study achieved an ACR 20 response at 24 weeks (P = 0.71). Sixty-nine percent of patients receiving IFN-β and 67% receiving placebo terminated the study early, most of them secondary to a perceived lack of efficacy. Overall, IFN-β-1a had a safety profile similar to that of placebo. There were no significant differences in the proportion of patients achieving an ACR 20 response between the two groups

PhyleasProg: a user-oriented web server for wide evolutionary analyses

Evolutionary analyses of biological data are becoming a prerequisite in many fields of biology. At a time of high-throughput data analysis, phylogenetics is often a necessary complementary tool for biologists to understand, compare and identify the functions of sequences. But available bioinformatics tools are frequently not easy for non-specialists to use. We developed PhyleasProg (http://phyleasprog.inra.fr), a user-friendly web server as a turnkey tool dedicated to evolutionary analyses. PhyleasProg can help biologists with little experience in evolutionary methodologies by analysing their data in a simple and robust way, using methods corresponding to robust standards. Via a very intuitive web interface, users only need to enter a list of Ensembl protein IDs and a list of species as inputs. After dynamic computations, users have access to phylogenetic trees, positive/purifying selection data (on site and branch-site models), with a display of these results on the protein sequence and on a 3D structure model, and the synteny environment of related genes. This connection between different domains of phylogenetics opens the way to new biological analyses for the discovery of the function and structure of proteins

Generation of a near infra-red guide star catalog for thirty-meter telescope observations

The requirements for the production of a near Infra-Red Guide Star Catalog (IRGSC) for Thirty Meter Telescope (TMT) observations are identified and presented. A methodology to compute the expected J band magnitude of stellar sources from their optical (g, r, i) magnitudes is developed. The computed and observed J magnitudes of sources in three test fields are compared and the methodology developed is found to be satisfactory for the magnitude range, JVega = 16–22 mag. From this analysis, we found that for the production of final TMT IRGSC (with a limiting magnitude of JVega = 22 mag), we need g, r, i bands optical data which go up to iAB ~ 23 mag. Fine tuning of the methodology developed, such as using Spectral Energy Distribution (SED) template fitting for optimal classification of stars in the fainter end, incorporating spectral libraries in the model, to reduce the scatter, and modification of the existing colour–temperature relation to increase the source density are planned for the subsequent phase of this work

A road map for the generation of a near-infrared guide star catalog for thirty meter telescope observations

The near-infrared instruments in the upcoming Thirty Meter Telescope (TMT) will be assisted by a multi conjugate Adaptive Optics (AO) system. For the efficient operation of the AO system, during observations, a near-infrared guide star catalog which goes as faint as 22 mag in JVega band is essential and such a catalog does not exist. A methodology, based on stellar atmospheric models, to compute the expected near-infrared magnitudes of stellar sources from their optical magnitudes is developed. The method is applied and validated in JHKs bands for a magnitude range of JVega 16–22 mag. The methodology is also applied and validated using the reference catalog of PAN STARRS. We verified that the properties of the final PAN STARRS optical catalog will satisfy the requirements of TMT IRGSC and will be one of the potential sources for the generation of the final catalog. In a broader context, this methodology is applicable for the generation of a guide star catalog for any existing/upcoming near-infrared telescopes

The cohesin ring concatenates sister DNA molecules

Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit protein complex called cohesin whose Scc1, Smc1, and Smc3 subunits form a tripartite ring structure. It has been proposed that cohesin holds sister DNAs together by trapping them inside its ring. To test this, we used site-specific cross-linking to create chemical connections at the three interfaces between the ring’s three constituent polypeptides, thereby creating covalently closed cohesin rings. As predicted by the ring entrapment model, this procedure produces dimeric DNA/cohesin structures that are resistant to protein denaturation. We conclude that cohesin rings concatenate individual sister minichromosome DNAs

Evaluation of a Hybrid Boltzmann-Continuum Method for High Speed Nonequilibrium Flows

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/83556/1/AIAA-2010-1569-683.pd

Synthesis of Newly Formulated Aluminium Composite through Powder Metallurgy using Waste Bone Material

The increasing concern for sustainable materials and waste management has led to innovative approaches in material science. This study explores the potential benefit of aggregate waste in the production of aluminum composites practicing powder metallurgy techniques. The aim is to investigate the feasibility of incorporating bone material into aluminium matrices to enhance the composite’s mechanical properties. The research involves several key steps. Firstly, waste bone material is collected and processed to obtain a fine powder suitable for powder metallurgy. Various techniques such as grinding, milling, or pulverization are employed to achieve the desired particle size distribution. Next, the bone powder is mixed with aluminium powder in predetermined ratios to create composite blends. The composite blends are then subjected to compaction using powder metallurgy techniques, including cold pressing and sintering. The compaction process aims to consolidate the powders and facilitate the formation of a solid composite structure. The aluminum composites mechanical characteristics are then assessed. The effects of incorporating bone material are assessed using tests on tensile strength, ductility, hardness, and other relevant mechanical properties. Comparative analysis is performed between the composites with bone material and traditional aluminium composites to assess any improvements or changes in performance