Self-Learning Cloud Controllers: Fuzzy Q-Learning for Knowledge Evolution

Cloud controllers aim at responding to application demands by automatically scaling the compute resources at runtime to meet performance guarantees and minimize resource costs. Existing cloud controllers often resort to scaling strategies that are codified as a set of adaptation rules. However, for a cloud provider, applications running on top of the cloud infrastructure are more or less black-boxes, making it difficult at design time to define optimal or pre-emptive adaptation rules. Thus, the burden of taking adaptation decisions often is delegated to the cloud application. Yet, in most cases, application developers in turn have limited knowledge of the cloud infrastructure. In this paper, we propose learning adaptation rules during runtime. To this end, we introduce FQL4KE, a self-learning fuzzy cloud controller. In particular, FQL4KE learns and modifies fuzzy rules at runtime. The benefit is that for designing cloud controllers, we do not have to rely solely on precise design-time knowledge, which may be difficult to acquire. FQL4KE empowers users to specify cloud controllers by simply adjusting weights representing priorities in system goals instead of specifying complex adaptation rules. The applicability of FQL4KE has been experimentally assessed as part of the cloud application framework ElasticBench. The experimental results indicate that FQL4KE outperforms our previously developed fuzzy controller without learning mechanisms and the native Azure auto-scaling

Form-finding and Buckling Optimization of Grid Shells using genetic Algorithms

We present a strategy for the design of gridshells where form-found structures are optimised for buckling resistance. A genetic algorithm is employed for the initialisation of pre-stress forces required in form-finding using dynamic relaxation. Dynamic relaxation takes this initial pre-stress, a flat grid, as well as self-weight and nodal loads to calculate a static equilibrium. The structure is then analysed for the estimation of the critical buckling load. Different boundary conditions, structural parameters and typology of connections are compared, including a gridshells with triangular and quadrangular patterns. Optimised structures are measured against the trivial solution, which is a structure where dynamic relaxation is initialised with uniform pre-stress. Our results show the proposed strategy can successfully form find gridshells with improved buckling performance

Hemp Growth Factors and Extraction Methods Effect on Antimicrobial Activity of Hemp Seed Oil: A Systematic Review

The bioactive Hemp Seed Oil (HSO) is becoming very popular in the medical and research fields due to its antimicrobial properties against several diseases caused by bacteria and fungi. However, the effect of hemp-growing factors and extraction methods on the bioactivity of HSO does not receive adequate research attention. Therefore, this review aims to investigate the effect of growth factors and extraction methods on the antimicrobial activity of HSO. Articles were retrieved from Google Scholar and the Scopus database and screened against inclusion and exclusion criteria. The study revealed that HSO prefers warm climates and favorable humidity ranging from 20 to 39 °C and 79–100% per year, respectively, and rainfall of 324 mm daily. The multivariate linear regression shown excellent prediction (R2 = 0.94) with climates upon Zone of Growth Inhibition (ZGI) of Gram-positive bacteria. Temperature is the strongest predictor (p \u3c 0.01) followed by humidity and rainfall (p \u3c 0.05). Furthermore, well-drained loam soil rich in organic matter seems to stimulate the antimicrobial activity of HSO. The major constituents that influence HSO’s antimicrobial ability to Staphylococcus aureus were cannabidiol (CBD), β-caryophyllene, and limonene. The extraction methods showed less influence on the HSO bioactivity. HSO did not show significant antioxidant activity, but Hemp Seed Hull (HSH), Hemp Seed Flour (HSF), and Hydrolyzed Hemp Seed Protein (HPH), expressed promising DPPH scavenging ability

Multifunctional Nanocarriers for diagnostics, drug delivery and targeted treatment across blood-brain barrier: perspectives on tracking and neuroimaging

Nanotechnology has brought a variety of new possibilities into biological discovery and clinical practice. In particular, nano-scaled carriers have revolutionalized drug delivery, allowing for therapeutic agents to be selectively targeted on an organ, tissue and cell specific level, also minimizing exposure of healthy tissue to drugs. In this review we discuss and analyze three issues, which are considered to be at the core of nano-scaled drug delivery systems, namely functionalization of nanocarriers, delivery to target organs and in vivo imaging. The latest developments on highly specific conjugation strategies that are used to attach biomolecules to the surface of nanoparticles (NP) are first reviewed. Besides drug carrying capabilities, the functionalization of nanocarriers also facilitate their transport to primary target organs. We highlight the leading advantage of nanocarriers, i.e. their ability to cross the blood-brain barrier (BBB), a tightly packed layer of endothelial cells surrounding the brain that prevents high-molecular weight molecules from entering the brain. The BBB has several transport molecules such as growth factors, insulin and transferrin that can potentially increase the efficiency and kinetics of brain-targeting nanocarriers. Potential treatments for common neurological disorders, such as stroke, tumours and Alzheimer's, are therefore a much sought-after application of nanomedicine. Likewise any other drug delivery system, a number of parameters need to be registered once functionalized NPs are administered, for instance their efficiency in organ-selective targeting, bioaccumulation and excretion. Finally, direct in vivo imaging of nanomaterials is an exciting recent field that can provide real-time tracking of those nanocarriers. We review a range of systems suitable for in vivo imaging and monitoring of drug delivery, with an emphasis on most recently introduced molecular imaging modalities based on optical and hybrid contrast, such as fluorescent protein tomography and multispectral optoacoustic tomography. Overall, great potential is foreseen for nanocarriers in medical diagnostics, therapeutics and molecular targeting. A proposed roadmap for ongoing and future research directions is therefore discussed in detail with emphasis on the development of novel approaches for functionalization, targeting and imaging of nano-based drug delivery systems, a cutting-edge technology poised to change the ways medicine is administered

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Aim: To investigate the influence of gold nanoparticle (GNP) geometry on the biochemical response of Calu-3 epithelial cells. Materials and Methods: Spherical, triangular and hexagonal GNPs were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72hrs at [0.0001-1mg/mL]. Results: At 1mg/mL, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of pro-apoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose

Knowledge-defined networking

The research community has considered in the past the application of Artificial Intelligence (AI) techniques to control and operate networks. A notable example is the Knowledge Plane proposed by D.Clark et al. However, such techniques have not been extensively prototyped or deployed in the field yet. In this paper, we explore the reasons for the lack of adoption and posit that the rise of two recent paradigms: Software-Defined Networking (SDN) and Network Analytics (NA), will facilitate the adoption of AI techniques in the context of network operation and control. We describe a new paradigm that accommodates and exploits SDN, NA and AI, and provide use-cases that illustrate its applicability and benefits. We also present simple experimental results that support, for some relevant use-cases, its feasibility. We refer to this new paradigm as Knowledge-Defined Networking (KDN).Peer ReviewedPostprint (author's final draft

Pesticidas, residualidad y períodos de carencia : aplicaciones en el cultivo del aguacate

Guía en la que se conceptualizan temas asociados a uso de plaguicidas, periodos de carencia y determinación de residualidad química aplicables al cultivo de aguacateGuide in which topics related to the use of pesticides, periods of deficiency and determination of chemical residuals applicable to the cultivation of avocado are conceptualizedPlaguicidas o pesticidas? -- Del codex alimentarius y su utilidad -- Del codex y los plaguicidas -- De las buenas prácticas agrícolas y el uso de plaguicidas -- De los plaguicidas y el ambiente -- De los períodos de carencia de un plaguicida -- De los métodos analíticos para la determinación y cuantificación de plaguicidasnaProducto derivado del Proyecto de Investigación Aplicada “Determinación de Períodos de carencia para plaguicidas de uso común en cultivos de aguacate Hass en el Oriente Antioqueño”. Proyecto Financiado por el Sistema de Investigación, Desarrollo Tecnológico e Innovación – SENNOVA del SENA.52 página

Cathepsin K induces platelet dysfunction and affects cell signaling in breast cancer - molecularly distinct behavior of cathepsin K in breast cancer

Background: Breast cancer comprises clinically and molecularly distinct tumor subgroups that differ in cell histology and biology and show divergent clinical phenotypes that impede phase III trials, such as those utilizing cathepsin K inhibitors. Here we correlate the epithelial-mesenchymal-like transition breast cancer cells and cathepsin K secretion with activation and aggregation of platelets. Cathepsin K is up-regulated in cancer cells that proteolyze extracellular matrix and contributes to invasiveness. Although proteolytically activated receptors (PARs) are activated by proteases, the direct interaction of cysteine cathepsins with PARs is poorly understood. In human platelets, PAR-1 and -4 are highly expressed, but PAR-3 shows low expression and unclear functions. Methods: Platelet aggregation was monitored by measuring changes in turbidity. Platelets were immunoblotted with anti-phospho and total p38, Src-Tyr-416, FAK-Tyr-397, and TGF beta monoclonal antibody. Activation was measured in a flow cytometer and calcium mobilization in a confocal microscope. Mammary epithelial cells were prepared from the primary breast cancer samples of 15 women with Luminal-B subtype to produce primary cells. Results: We demonstrate that platelets are aggregated by cathepsin K in a dose-dependent manner, but not by other cysteine cathepsins. PARs-3 and -4 were confirmed as the cathepsin K target by immunodetection and specific antagonists using a fibroblast cell line derived from PARs deficient mice. Moreover, through co-culture experiments, we show that platelets activated by cathepsin K mediated the up-regulation of SHH, PTHrP, OPN, and TGF beta in epithelial-mesenchymal-like cells from patients with Luminal B breast cancer. Conclusions: Cathepsin K induces platelet dysfunction and affects signaling in breast cancer cells.Associacao Beneficente de Coleta de Sangue (Colsan)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Univ Fed Sao Paulo, Dept Gynecol, BR-04024002 Sao Paulo, SP, BrazilCOLSAN, Charitable Assoc Blood Collect, BR-04080006 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biophys, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biochem, BR-04024002 Sao Paulo, SP, BrazilAntonio Prudente Fdn, AC Camargo Canc Ctr, AC Camargo Hosp Biobank, Dept Pathol, BR-01509010 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Cellular Gynecol Lab, Dept Gynecol, Rua Napoleao Barros 608, BR-04024002 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Gynecol, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biophys, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biochem, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Cellular Gynecol Lab, Dept Gynecol, Rua Napoleao Barros 608, BR-04024002 Sao Paulo, BrazilFAPESP: 2012/19780-3FAPESP: 2012/19851-8FAPESP: 2009/53766-5Web of Scienc