Development of a molecular method for enumerating viable Ascaris eggs from feacal sludge

La majorité de la population urbaine des pays en voie de développement dépend de systèmes assainissement autonomes produisant des boues de vidange dont le contenu en pathogènes représente un risque important pour l'environnement et la santé. Le projet du groupe du professeur Holliger de I'EPFL vise à évaluer et optimiser le traitement des boues de vidange par digestion anaérobie. Comme organisme modèle pour cette analyse, les oeufs d'helminlhe (vers qui infectent les porcs) ont été choisis. Dans le cadre de son travail de diplôme, Mme Antsa Rabenifara aura à établir une méthode moléculaire de quantification des oeufs d'helminthe sur la base d'une étude publiée en 2006 par Pecson et al. permettant un suivi optimal des pathogènes et de leur viabilité lors des tests d'inactivation.This study is a part of a project that aims to set up a molecular method to evaluate the viability of Ascaris eggs during sludge treatment. The specific objectives of the thesis is to develop a method for breaking eggshell prior to nucleic acid extraction and to design and optimize a qPCR method to quantify viable Ascaris eggs

An Initial Study on How Families Deal with Ridicule and Being Laughed at: Parenting Styles and Parent-Child Relations with Respect to Gelotophobia, Gelotophilia, and Katagelasticism

Familial aggregation and the effect of parenting styles on three dispositions toward ridicule and being laughed at were tested. Nearly 100 families (parents, their adult children, and their siblings) completed subjective questionnaires to assess the presence of gelotophobia (the fear of being laughed at), gelotophilia (the joy of being laughed at), and katagelasticism (the joy of laughing at others). A positive relationship between fear of being laughed at in children and their parents was found. Results for gelotophilia were similar but numerically lower; if split by gender of the adult child, correlations to the mother's gelotophilia exceeded those of the father. Katagelasticism arose independently from the scores in the parents but was robustly related to greater katagelasticism in the children's siblings. Gelotophobes remembered punishment (especially from the mother), lower warmth and higher control from their parents (this was also found in the parents' recollections of their parenting style). The incidence of gelotophilia was unrelated to specific parenting styles, and katagelasticism exhibited only weak relations with punishment. The study suggests a specific pattern in the relation of the three dispositions within families and argues for a strong impact of parenting styles on gelotophobia but less so for gelotophilia and katagelasticis

Breaking barriers in passive sampling: the potential of PTFE membranes in the monitoring of hydrophilic micropollutants

Passive samplers are key tools to sample hydrophilic micropollutants in water. Two main approaches address hydrodynamics’ influence: (1) determining site-specific sampling rate (RS) by characterizing kw, the mass transfer coefficient of the water-boundary layer (WBL), and (2) reducing WBL impact using a diffusive material to control the uptake. The first requires calibration data and the second has only been achieved using fragile diffusive material. This study assesses the transfer of hydrophilic contaminants through polytetrafluoroethylene (PTFE; 30 μm thick), a new membrane material with lower sorption than commonly used polyethersulfone (PES). Combined for the first time in a Chemcatcher-like configuration, we calibrated the modified samplers for 44 micropollutants to provide RS – kw relationships for in-situ RS determination (approach 1). Micropollutants accumulated over 2000 times more on the sorbent than on PTFE. PTFE-based RS (0.027 to 0.300 L day-1) were 2.5 higher than previously reported with PES. Membrane property measurements (porosity, tortuosity) indicated that accumulation is primarily controlled by the membrane. Extrapolation indicated that using thicker PTFE membranes (≥ 100 μm) would shift uptake control entirely to the membrane in river conditions (approach 2). This finding could enable RS prediction based on contaminants properties, thus representing a significant advancement in passive sampling

An improved Chemcatcher-based method for the integrative passive sampling of 44 hydrophilic micropollutants in surface water – Part A: Calibration under four controlled hydrodynamic conditions

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Chronic lymphocytic leukaemia: clinical-aetiological findings in 66 patients and their families

<p>Abstract</p> <p>Background</p> <p>Little is known about the aetiology of chronic lymphocytic leukaemia (CLL). The family medical history is a "genomic tool" capturing interactions of genetic susceptibility, shared environment and common behaviours.</p> <p>Methods</p> <p>A cohort of 66 consecutives patients with CLL (probands) was studied in a medical oncology practice (W.W.) from 1981 until 2005. A German version of the NCI medical history questionnaire for cancer aetiology was used. Familial clustering analysis was done by comparing the proportion of specific tumours in the first degree relatives of the CLL practice cohort with corresponding proportions of population-based cancer registry data.</p> <p>Results</p> <p>18 (41%) male and 5 (23%) female CLL probands had multiple malignancies, e.g. 2 meningiomas, 7 and 19 years after diagnosis of CLL. 46 (12%) first degree relatives had malignancies with an excess of CLL. Other conspicuous familial associations are CLL with malignancies of the upper GI tract (oesophagus, stomach) and of the nervous system.</p> <p>Conclusion</p> <p>1. Chronic lymphocytic leukaemia clusters in some families like any other disease. Predisposition genes should be searched. 2. Cancer prevention and early detection should be continued in CLL patients because of their longevity and high risk for multiple malignancies. 3. The overrepresentation of upper GI malignancies in first degree relatives of CLL patients calls for targeted oesophago-gastroscopy screening studies.</p

An improved Chemcatcher-based method for the integrative passive sampling of 44 hydrophilic micropollutants in surface water – Part B: Field implementation and comparison with automated active sampling

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Consequences of gestational diabetes mellitus on neonatal cardiovascular health: MySweetHeart Cohort study.

BACKGROUND Hyperglycaemic disorders of pregnancy are associated with offspring cardiovascular alterations. METHODS MySweetHeart cohort study aimed to assess the effect of maternal gestational diabetes (GDM) on offsprings' cardiovascular health. Newborns underwent clinical and echocardiographic examinations between 2016 and 2020. RESULTS Compared to mothers without GDM (n = 141), mothers with GDM (n = 123) were more likely to have had GDM in previous pregnancies and had higher weight, BMI, blood glucose, and HbA1c. Newborns of both groups showed similar clinical characteristics. Echocardiography was performed on the 3rd (interquartile range, IQR, 2nd-4th) day of life in 101 offsprings of mothers without and 116 offsprings of mothers with GDM. Left ventricular (LV) mass was similar. Children born to mothers with GDM had a thicker posterior LV wall (z-score +0.15, IQR -0.38/0.62, versus +0.47, IQR -0.11/+1.1, p = 0.004), a smaller end-systolic (1.3 mL, IQR 1.0-1.5 mL, versus 1.4 mL, IQR 1.2-1.8 mL, p = 0.044) but a similar end-diastolic LV volume. They also had shorter tricuspid valve flow duration and aortic valve ejection time, lower tricuspid E-wave and pulmonary valve velocities. CONCLUSIONS Newborns of mothers with or without GDM had similar clinical characteristics and LV mass. However, some echocardiographic differences were detected, suggesting an altered myocardial physiology among infants of mothers with GDM. REGISTRATION ClinicalTrials.gov (NCT02872974). IMPACT Hyperglycaemic disorders of pregnancy are known to be associated with offspring cardiovascular alterations. Clinical characteristics and estimated left ventricular (LV) mass were similar in children issued from mothers with and without gestational diabetes (GDM). Children born to mothers with GDM had a thicker posterior LV wall and a smaller end-systolic LV volume. Although LV mass is not different, myocardial physiology may be altered in these infants. Further studies should investigate the endothelial function of this population and the cardiovascular evolution of these children over time

Gestational diabetes mellitus and offspring's carotid intima-media thickness at birth: MySweetHeart Cohort study.

OBJECTIVE Hyperglycaemia during pregnancy is associated with cardiometabolic risks for the mother and the offspring. Mothers with gestational diabetes mellitus (GDM) have signs of subclinical atherosclerosis, including increased carotid intima-media thickness (CIMT). We assessed whether GDM is associated with increased CIMT in the offspring at birth. DESIGN AND SETTING MySweetHeart Cohort is a prospective cohort study conducted in Switzerland. PARTICIPANTS, EXPOSURE AND OUTCOME MEASURES This work included pregnant women with and without GDM at 24-32 weeks of gestation and their singleton live-born offspring with data on the primary outcome of CIMT. GDM was diagnosed based on the criteria of the International Association of Diabetes and Pregnancy Study Groups. Offspring's CIMT was measured by ultrasonography after birth (range 1-19 days). RESULTS Data on CIMT were available for 99 offspring of women without GDM and 101 offspring of women with GDM. Maternal age ranged from 18 to 47 years. Some 16% of women with GDM and 6% of women without GDM were obese. Smoking during pregnancy was more frequent among women with GDM (18%) than among those without GDM (4%). Neonatal characteristics were comparable between the two groups. The difference in CIMT between offspring of women with and without GDM was of 0.00 mm (95% CI -0.01 to 0.01; p=0.96) and remained similar on adjustment for potential confounding factors, such as maternal prepregnancy body mass index, maternal education, smoking during pregnancy, family history of diabetes, as well as offspring's sex, age, and body surface area (0.00 mm (95% CI -0.02 to 0.01; p=0.45)). CONCLUSIONS We found no evidence of increased CIMT in neonates exposed to GDM. A longer-term follow-up that includes additional vascular measures, such as endothelial function or arterial stiffness, may shed further light on the cardiovascular health trajectories in children born to mothers with GDM. TRIAL REGISTRATION NUMBER NCT02872974; Pre-results