Schinus terebinthifolius Raddi ( Aroeira) leaves oil attenuates inflammatory responses in cutaneous wound healing in mice

Purpose: To investigated the inflammatory, angiogenic and fibrogenic activities of the Schinus terebinthifolius Raddi leaves oil (STRO) on wound healing. Methods: The excisional wound healing model was used to evaluate the effects of STRO. The mice were divided into two groups: Control, subjected to vehicle solution (ointment lanolin/vaseline base), or STRO-treated group, administered topically once a day for 3, 7 and 14 days post-excision. We evaluated the macroscopic wound closure ratethe inflammation was evaluated by leukocytes accumulation and cytokine levels in the wounds. The accumulation of neutrophil and macrophages in the wounds were determined by assaying myeloperoxidase and N-acetyl-beta-D-glucosaminidase activities. The levels of TNF-alpha, CXCL-1 and CCL-2 in wound were evaluated by ELISA assay. Angiogenesis and collagen fibers deposition were evaluated histologically. Results: We observed that macroscopic wound closure rate was improved in wounds from STRO-group than Control-group. The wounds treated with STRO promoted a reduction in leucocyte accumulation and in pro-inflammatory cytokine. Moreover, STRO treatment increased significantly the number of blood vessels and collagen fibers deposition, as compared to control group. Conclusion: Topical application of STRO display anti-inflammatory and angiogenic effects, as well as improvement in collagen replacement, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, including wound healing.Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Rural Pernambuco UFRPE, Dept Morphol & Anim Physiol, Recife, PE, BrazilUniv Fed São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilUniv Fed Rural Pernambuco, Dept Chem, Recife, PE, BrazilUniv Fed Rural Pernambuco, Dept Morphol & Anim Physiol, Recife, PE, BrazilUniv Fed São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilWeb of Scienc