Molecular species profile of the ganglioside classes of the retina and other ocular tissues, brain and plasma.

<p>Data (peak areas) were obtained by operating the QqQ mass spectrometer in negative SRM mode. The proportion of each ceramide species was expressed, as percentage, relatively to the sum of all detected species in its specific GG class, every GG class being considered separately. Molecular species accounting for less than 1% were grouped under the category “others”. Results were expressed as mean of 4 to 7 independent samples for each tissue, injected three times. The color intensity of a spot on the graph is proportional to the mean percentage of the ceramide species considered within a GG class.</p

Ganglioside profile of the retina and other ocular tissues, brain and plasma.

<p>A. Resorcinol-stained HPTLC plate of GGs extracted from retina, retinal pigment epithelium (RPE)/choroid, ciliary body, optic nerve, brain and plasma. GG aliquots (4–7 independent samples) were pooled for each tissue type to represent a total of 10 nmol GG-sialic acid spotted per lane. A standard mixture of ganglio-series GGs (Std, 5 nmol sialic acid) was also spotted. The plate was developed in CHCl₃/CH₃OH/0.2% CaCl₂ (55:45:10, v/v/v) and revealed with resorcinol reagent. B. Quantitative distribution of GG classes calculated from a standard curve of each GG class. Results are expressed in nmol GG/mg protein or nmol GG/mL plasma.</p

Ceramide proportions in retinal GG classes (A, C, E) and in retinal and brain GT3 and AcGT3 (B, D and F).

<p>Means of 4–7 independent samples and 95% confidence ellipses are represented on ternary diagrams for various groups of ceramides: (36:1 + 38:1), (40:1 + 42:2 + 42:1) ceramides and other molecular species (A and B); ceramides with 1, 2 and 3 unsaturations (C and D); ceramides with 34 carbons or less, 42 carbons or more and other molecular species (E and F). Axes from 0 to 1 for the three variables are represented on B and the proportions for retinal GT3 are shown as an example.</p

Ceramide proportions in the GG classes of the retina and other ocular tissues, brain and plasma.

<p>Ceramide proportions in the GG classes of the retina and other ocular tissues, brain and plasma.</p

Ceramide molecular species of the retinal ganglioside classes characterized by HRMS with the LTQ-Orbitrap mass spectrometer.

<p>Ceramide molecular species of the retinal ganglioside classes characterized by HRMS with the LTQ-Orbitrap mass spectrometer.</p