Hydrological Monitoring with Hybrid Sensor Networks

Existing hydrological monitoring systems suffer from short- comings in accuracy, resolution, and scalability. Their fragility, high power consumption, and lack of autonomy necessitate frequent site visits. Cabling requirements and large size limit their scalability and make them prohibitively expensive. The research described in this paper proposes to alleviate these problems by pairing high-resolution in situ measure- ment with remote data collection and software maintenance. A hybrid sensor network composed of wired and wireless connections autonomously measures various attributes of the soil, including moisture, temperature, and resistivity. The mea- surements are communicated to a processing server over the existing GSM cellular infrastructure. This system enables the collection of data at a scale and resolution that is orders of magnitude greater than any existing method, while dramatically reducing the cost of monitoring. The quality and sheer volume of data collected as a result will enable previously infeasible research in hydrology

Microbial Nanowires: Is the Subsurface Hardwired ?

The Earth\u27s shallow subsurface results from integrated biological, geochemical, and physical processes. Methods are sought to remotely assess these interactive processes, especially those catalysed by micro-organisms. Using saturated sand columns and the metal reducing bacterium Shewanella oneidensis MR-1, we show that electrically conductive appendages called bacterial nanowires are directly associated with electrical potentials. No significant electrical potentials were detectable in columns inoculated with mutant strains that produced non-conductive appendages. Scanning electron microscopy imaging revealed a network of nanowires linking cells-cells and cells to mineral surfaces, hardwiring the entire length of the column. We hypothesize that the nanowires serve as conduits for transfer of electrons from bacteria in the anaerobic part of the column to bacteria at the surface that have access to oxygen, akin to a biogeobattery. These results advance understanding of the mechanisms of electron transport in subsurface environments and of how microorganisms cycle geologic material and share energy

Microbial Growth and Biofilm Formation in Geologic Media Is Detected with Complex Conductivity Measurements

Complex conductivity measurements (0.1-1000 Hz) were obtained from biostimulated sand-packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and biofilms to sand surfaces. We conclude that complex conductivity techniques, specifically imaginary conductivity measurements are a proxy indicator for microbial growth and biofilm formation in porous media. Our results have implications for microbial enhanced oil recovery, CO2 sequestration, bioremediation, and astrobiology studies

Thermal Perturbations beneath the Incipient Okavango Rift Zone, Northwest Botswana

We used aeromagnetic and gravity data to investigate the thermal structure beneath the incipient Okavango Rift Zone (ORZ) in northwestern Botswana in order to understand its role in strain localization during rift initiation. We used three-dimensional (3-D) inversion of aeromagnetic data to estimate the Curie Point Depth (CPD) and heat flow under the rift and surrounding basement. We also used two-dimensional (2-D) power-density spectrum analysis of gravity data to estimate the Moho depth. Our results reveal shallow CPD values (8-15 km) and high heat flow (60-90 mW m-2) beneath a ∼60 km wide NE-trending zone coincident with major rift-related border faults and the boundary between Proterozoic orogenic belts. This is accompanied by thin crust ( \u3c 30 km) in the northeastern and southwestern parts of the ORZ. Within the Precambrian basement areas, the CPD values are deeper (16-30 km) and the heat flow estimates are lower (30-50 mW m-2), corresponding to thicker crust (∼40-50 km). We interpret the thermal structure under the ORZ as due to upward migration of hot mantle fluids through the lithospheric column that utilized the presence of Precambrian lithospheric shear zones as conduits. These fluids weaken the crust, enhancing rift nucleation. Our interpretation is supported by 2-D forward modeling of gravity data suggesting the presence of a wedge of altered lithospheric mantle centered beneath the ORZ. If our interpretation is correct, it may result in a potential paradigm shift in which strain localization at continental rift initiation could be achieved through fluid-assisted lithospheric weakening without asthenospheric involvement

Thermal Perturbations beneath the Incipient Okavango Rift Zone, Northwest Botswana

We used aeromagnetic and gravity data to investigate the thermal structure beneath the incipient Okavango Rift Zone (ORZ) in northwestern Botswana in order to understand its role in strain localization during rift initiation. We used three-dimensional (3-D) inversion of aeromagnetic data to estimate the Curie Point Depth (CPD) and heat flow under the rift and surrounding basement. We also used two-dimensional (2-D) power-density spectrum analysis of gravity data to estimate the Moho depth. Our results reveal shallow CPD values (8-15 km) and high heat flow (60-90 mW m-2) beneath a ∼60 km wide NE-trending zone coincident with major rift-related border faults and the boundary between Proterozoic orogenic belts. This is accompanied by thin crust ( \u3c 30 km) in the northeastern and southwestern parts of the ORZ. Within the Precambrian basement areas, the CPD values are deeper (16-30 km) and the heat flow estimates are lower (30-50 mW m-2), corresponding to thicker crust (∼40-50 km). We interpret the thermal structure under the ORZ as due to upward migration of hot mantle fluids through the lithospheric column that utilized the presence of Precambrian lithospheric shear zones as conduits. These fluids weaken the crust, enhancing rift nucleation. Our interpretation is supported by 2-D forward modeling of gravity data suggesting the presence of a wedge of altered lithospheric mantle centered beneath the ORZ. If our interpretation is correct, it may result in a potential paradigm shift in which strain localization at continental rift initiation could be achieved through fluid-assisted lithospheric weakening without asthenospheric involvement

Effect of Bacterial Adsorption on Low Frequency Electrical Properties of Clean Quartz Sands and Iron-Oxide Coated Sands

Low frequency electrical measurements (0.1-1000 Hz) were conducted to investigate the adsorption effect of Pseudomonas aeruginosa cells onto clean quartz sands and iron-oxide coated sands. The clean quartz sands showed a gradual increase in the microbial adsorption to mineral grains, concurrent with an increase of 13% in the imaginary conductivity component (σ″). However, iron-oxide coated sands (20-100% by weight) showed a rapid increase in microbial adsorption with σ″ reaching a maximum of 37% for the 80-100% iron coated sands. No significant changes were observed in the real conductivity component (σ′) due to microbial adsorption. A power law dependency was observed between the adsorbed cells and σ″. We suggest that the polarization results from the increase in the surface roughness and surface area of the grain due to bacteria sorption. These results suggest that low frequency electrical measurements can play an important role in assessing microbial transport in subsurface environments

Sensitivity of Geoelectrical Measurements to the Presence of Bacteria in Porous Media

We investigated the sensitivity of low-frequency electrical measurements (0.1-1000 Hz) to (1) microbial cell density, (2) live and dead cells, and (3) microbial attachment onto mineral surfaces of clean quartz sands and iron oxide-coated sands. Three strains of Pseudomonas aeruginosa PAO1 (wild type and rhlA and pilA mutant) with different motility and attachment properties were used. Varying concentrations of both live and dead cells of P. aeruginosa wild type in sand columns showed no effect on the real conductivity component (σ′). However, the imaginary conductivity component (σ″) increased linearly with increasing concentrations of live cells in sand columns, whereas minimal changes were observed with different concentrations of dead cells. A strong power law relationship was observed between σ″ and the number of cells adsorbed onto sand grain surfaces with the rhlA mutant of P. aeruginosa displaying a higher power law exponent compared to the wild type and pilA mutant. In addition, power law exponents were greater in columns with iron oxide-coated sands compared to clean quartz sands. Minimal changes were observed on the σ′ due to the attachment of P. aeruginosa cells onto sands. We relate the measured low-frequency electrical responses to (1) the distinct electrical properties of live cells and (2) the density of cells attached to mineral surfaces enhancing the surface roughness of sand grains and hence the polarization response. The information obtained from this study enhances our interpretation of microbially induced geoelectrical responses in biostimulated geologic media and may have implications for microbial transport studies