Ohio Pastures for Profit Online

The Pastures for Profit program is an established course created through a collaboration between Ohio State University Extension, Central State University, USDA-Natural Resources Conservation Service, Ohio Federation of Soil and Water Conservation Districts, Ohio Department of Agriculture, and the Ohio Forage and Grasslands Council. In 2021, due to COVID-19, the Pastures for Profit curriculum was adapted to a virtual classroom setting and was offered again in 2022. The virtual experience consisted of three live webinars pairing with corresponding modules in Scarlet Canvas. Enrolled students were also mailed traditional course materials for personal use. Module content was newly developed and compiled to compliment the traditional materials and included videos, quizzes, additional presentations, and technical resources. The course materials were organized and taught by a team of over 50 collaborators. During the three live webinars, nearly 20 speakers presented on topics including the basics of grazing, plant and animal science, and grazing plans. A total of 168 people enrolled in Scarlet Canvas in the two-year time frame from over 15 states and included producers, educators, technical service providers, and government agency staff. Each live session was recorded and made available to the class for access at their convenience. Those who completed the course obtained a signed certificate of completion. This platform allowed for an expanded audience reach than in the past

IL18-containing 5-gene signature distinguishes histologically identical dermatomyositis and lupus erythematosus skin lesions

Skin lesions in dermatomyositis (DM) are common, are frequently refractory, and have prognostic significance. Histologically, DM lesions appear similar to cutaneous lupus erythematosus (CLE) lesions and frequently cannot be differentiated. We thus compared the transcriptional profile of DM biopsies with CLE lesions to identify unique features. Type I IFN signaling, including IFN-κ upregulation, was a common pathway in both DM and CLE; however, CLE also exhibited other inflammatory pathways. Notably, DM lesions could be distinguished from CLE by a 5-gene biomarker panel that included IL18 upregulation. Using single-cell RNA-sequencing, we further identified keratinocytes as the main source of increased IL-18 in DM skin. This study identifies a potentially novel molecular signature, with significant clinical implications for differentiating DM from CLE lesions, and highlights the potential role for IL-18 in the pathophysiology of DM skin disease

Motivation and context of concurrent stimulant and opioid use among persons who use drugs in the rural United States: A multi-site qualitative inquiry

Background: In recent years, stimulant use has increased among persons who use opioids in the rural U.S., leading to high rates of overdose and death. We sought to understand motivations and contexts for stimulant use among persons who use opioids in a large, geographically diverse sample of persons who use drugs (PWUD) in the rural settings. Methods: We conducted semi-structured individual interviews with PWUD at 8 U.S. sites spanning 10 states and 65 counties. Content areas included general substance use, injection drug use, changes in drug use, and harm reduction practices. We used an iterative open-coding process to comprehensively itemize and categorize content shared by participants related to concurrent use. Results: We interviewed 349 PWUD (64% male, mean age 36). Of those discussing current use of stimulants in the context of opioid use (n = 137, 39%), the stimulant most used was methamphetamine (78%) followed by cocaine/crack (26%). Motivations for co-use included: 1) change in drug markets and cost considerations; 2) recreational goals, e.g., seeking stronger effects after heightened opioid tolerance; 3) practical goals, such as a desire to balance or alleviate the effects of the other drug, including the use of stimulants to avoid/reverse opioid overdose, and/or control symptoms of opioid withdrawal; and 4) functional goals, such as being simultaneously energized and pain-free in order to remain productive for employment. Conclusion: In a rural U.S. cohort of PWUD, use of both stimulants and opioids was highly prevalent. Reasons for dual use found in the rural context compared to urban studies included changes in drug availability, functional/productivity goals, and the use of methamphetamine to offset opioid overdose. Education efforts and harm reduction services and treatment, such as access to naloxone, fentanyl test strips, and accessible drug treatment for combined opioid and stimulant use, are urgently needed in the rural U.S. to reduce overdose and other adverse outcomes.</p

Association of methamphetamine and opioid use with nonfatal overdose in rural communities.

Importance: Overdoses continue to increase in the US, but the contribution of methamphetamine use is understudied in rural communities. Question How frequently are methamphetamine and opioid use associated with nonfatal overdose in rural communities? Findings: In this cross-sectional, multistate study of rural communities, 79% of people using drugs reported past-30-day methamphetamine use; nonfatal overdose was greatest in people using both methamphetamine and opioids (22%) vs opioids alone (14%), or methamphetamine alone (6%). People using both substances reported the least access to treatment; only 17% of those using methamphetamine alone had naloxone. Meaning::These findings suggest that harm reduction and substance use disorder treatment interventions must address methamphetamine use as well as opioids to decrease overdose in rural communities