Summary of quantitative morphological analysis.

<p>Summary of quantitative morphological analysis.</p

Effects of filamentous actin tracking probes on the morphology of primary mouse hippocampal neurons [EGFP control and Lifeact-GFP(1)].

<p>Representative images of neurons transfected with EGFP (A-D) or Lifeact-GFP(1) (E-H). (A, E) expressed probes; (B, F) axonal marker Tau1; (C, G) pan-neuronal β3-tubulin; (D, H) merged images. Scale bar = 50μm.</p

Quantitative analysis of axonal morphology at DIV3 after transfection with EGFP, Lifeact-GFP(1), Lifeact-GFP(2), Lifeact-EGFP, Lifeact-7-mEGFP, Utr261-EGFP and F-tractin-EGFP expressing constructs.

<p>Neurons transfected with Lifeact-GFP(1) show decreased total axon length (A), decreased primary axon total length (B) and decreased total length of primary axon branches (D), compared to EGFP control. Neurons transfected with Lifeact-EGFP and Utr261-EGFP show also show decreased primary axon total length (B). Neurons transfected with Lifeact-GFP(2), Lifeact-7-mEGFP and F-tractin-EGFP did not show any significant changes in axonal morphology when compared to EGFP control (A-F). Between 17 and 47 cells, collected from at least 3 biological replicates, were analysed per construct. Error bars represent standard error of the mean. Significance was determined by Kruskal-Wallis Test (non-parametric one-way ANOVA) and Dunn's multiple corrections test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.</p

Quantitative analysis of dendritic morphology at DIV3 after transfection with EGFP, Lifeact-GFP(1), Lifeact-GFP(2), Lifeact-EGFP, Lifeact-7-mEGFP, Utr261-EGFP and F-tractin-EGFP expressing constructs.

<p>Compared to EGFP control, neurons transfected with Lifeact-GFP(2) showed significant decreases in the total length of dendritic trees (B), the mean length of primary dendritic shaft (C), the mean length of primary dendritic branches (E) and the mean length of dendritic trees (H). Neurons transfected with Utr261-EGFP showed an increase in the number of primary dendritic branches (D) and mean number of primary dendritic branches per dendritic tree per cell (G), compared to EGFP control. Neurons transfected with Lifeact-GFP(1), Lifeact-EGFP, Lifeact-7-mEGFP and F-tractin-EGFP did not show any significant changes in dendritic morphology when compared to EGFP control (A-H) Between 17 and 47 cells, collected from at least 3 biological replicates, were analysed per construct. Error bars represent standard error of the mean. Significance was determined by Kruskal-Wallis Test (non-parametric one-way ANOVA) and Dunn's multiple corrections test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.</p