Aspects of formation of educational electronic resources for cadets of high schools of the ministry of internal affairs of the Russian Federation

The article is devoted to the features of working out of the educational electronic resources used at studying the professional-focused course of computer science for preparation of militiamen

ИСПОЛЬЗОВАНИЕ МНОГОУРОВНЕВОЕ ИМИТАЦИОННЫЕ МОДЕЛИ РЕГЕНЕРИРУЮЩИХ ПРОЦЕССОВ ПРИ ОЦЕНКЕ РИТМИЧНОСТИ ПРОИЗВОДСТВЕННЫХ ПРОЦЕССОВ

The majority of modern methods aimed at calculating the evaluation grade on the basis of imitation experiments, assume that the output process is stationary. Therefore, the transitional period is rejected.Analytical Methods for the study are compact enough to obtain analytical expressions that can be used to study the behavior of the entire system or some nodes in the form of some functional.The present article analyses the precision of the evaluation grade, with resetting the statistics which was accumulated on the initial stage of the modeling process.DOI: http://dx.doi.org/10.12731/2227-930X-2013-3-2Большинство современных методов, направленных на расчете класс оценки на основе имитационных экспериментов, предположим, что процесс вывода является стационарным. Таким образом, переходный период будет отклонено.Аналитические методы для изучения достаточно компактны, чтобы получить аналитические выражения, которые могут быть использованы для изучения поведения всей системы или некоторые узлы в виде некоторого функционала.Настоящая статья анализирует точность классе оценки, с регулировкой статистику, которая была накоплена на начальном этапе процесса моделирования

Groups of Geomicrobiological Indicators Are Spread across Gas-Hydrate and Non-Gas-Hydrate Areas in the Northern Part of the Sea of Japan

The bioindication of oil and gas fields is a field of geomicrobiology that is mainly devoted to the detection of hydrocarbon-oxidizing microbial indicator species or functional genes in total DNA. However, it appears promising to use the physiological properties of microorganisms detection deposit type of hydrocarbons, in particular their ability to oxidize hydrocarbons under aerobic and anaerobic conditions. In this study, the most promising approach in this area was the method used for assessing the anaerobic degradation of hydrocarbons. When comparing molecular genetics and cultured methods of bioindication, it can be concluded that molecular biomarkers of functional genes for the anaerobic destruction of hydrocarbons (masD) make it possible to separate areas with traditional and gas-hydrate types of deposits. Using cultured methods, we found that representatives of the Nocardiaceae family of the phylum Actinomycetota were tied to the areas where gas hydrates were found. The ability of aerobic and facultative anaerobic hydrocarbon-oxidizing microorganisms to anaerobically utilize hydrocarbons was determined with cultured methods. For the first time, this ability was revealed for the genera Stenotrophomonas, Psychrobacter, Micrococcus and Peribacillus. The wide distribution of this ability that we found in strains isolated from both study regions suggests its prominent role in the destruction of hydrocarbons in marine sediments

Horizontal Transfer of Virulence Factors by Pathogenic Enterobacteria to Marine Saprotrophic Bacteria during Co-Cultivation in Biofilm

Environmental problems associated with marine pollution and climate warming create favorable conditions for the penetration and survival of pathogenic bacteria in marine ecosystems. These microorganisms have interspecific competitive interactions with marine bacteria. Co-culture, as an important research strategy that mimics the natural environment of bacteria, can activate silent genes or clusters through interspecies interactions. The authors used modern biotechnology of co-cultivation to dynamically study intercellular interactions between different taxa of bacteria—pathogenic enterobacteria Yersinia pseudotuberculosis and Listeria monocytogenes and saprotrophic marine bacteria Bacillus sp. and Pseudomonas japonica isolated in summer from the coastal waters of the recreational areas of the Sea of Japan. The results of the experiments showed that during the formation of polycultural biofilms, horizontal transfer of genes encoding some pathogenicity factors from Y. pseudotuberculosis and L. monocytogenes to marine saprotrophic bacteria with different secretion systems is possible. It was previously thought that this was largely prevented by the type VI secretion system (T6SS) found in marine saprotrophic bacteria. The authors showed for the first time the ability of marine bacteria Bacillus sp. and P. japonica to biofilm formation with pathogenic enterobacteria Y. pseudotuberculosis and L. monocytogenes, saprophytic bacteria with type III secretion system (T3SS). For the first time, a marine saprotrophic strain of Bacillus sp. Revealed manifestations of hyaluronidase, proteolytic and hemolytic activity after cultivation in a polycultural biofilm with listeria. Saprotrophic marine bacteria that have acquired virulence factors from pathogenic enterobacteria, including antibiotic resistance genes, could potentially play a role in altering the biological properties of other members of the marine microbial community. In addition, given the possible interdomain nature of intercellular gene translocation, acquired virulence factors can be transferred to marine unicellular and multicellular eukaryotes. The results obtained contribute to the paradigm of the epidemiological significance and potential danger of anthropogenic pollution of marine ecosystems, which creates serious problems for public health and the development of marine culture as an important area of economic activity in coastal regions