Resistência adesiva de uma nanocerâmica/resina com diferentes tratamentos de superfície

Poster apresentado no XXXIV Congresso Anual da Sociedade Portuguesa de Estomatologia e Medicina Dentária (SPEMD), 9-11 Outubro 2014, Auditórios do Centro Hospitalar e Universitário de Coimbra.Under a Creative Commons license - http://creativecommons.org/licenses/by-nc-nd/3.0

Application of LDH assay for therapeutic efficacy evaluation of ex vivo tumor models

Funding Information: This work was supported by AbbVie and by iNOVA4Health – UIDB/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia (FCT) / Ministério da Educação e Ciência, through national funds. RM and TFM were recipients of PhD fellowships funded by FCT (SFRH/BD/132163/2017 and PD/BD/128377/2017, respectively) and CB was funded by “The Discoveries Centre for Regenerative and Precision Medicine” (European Commission Horizon 2020 Research and Innovation programme, under the Grant Agreement 739572). Publisher Copyright: © 2021, The Author(s).The current standard preclinical oncology models are not able to fully recapitulate therapeutic targets and clinically relevant disease biology, evidenced by the 90% attrition rate of new therapies in clinical trials. Three-dimensional (3D) culture systems have the potential to enhance the relevance of preclinical models. However, the limitations of currently available cellular assays to accurately evaluate therapeutic efficacy in these models are hindering their widespread adoption. We assessed the compatibility of the lactate dehydrogenase (LDH) assay in 3D spheroid cultures against other commercially available readout methods. We developed a standardized protocol to apply the LDH assay to ex vivo cultures, considering the impact of culture growth dynamics. We show that accounting for growth rates and background release levels of LDH are sufficient to make the LDH assay a suitable methodology for longitudinal monitoring and endpoint assessment of therapeutic efficacy in both cell line-derived xenografts (xenospheres) and patient-derived explant cultures. This method has the added value of being non-destructive and not dependent on reagent penetration or manipulation of the parent material. The establishment of reliable readout methods for complex 3D culture systems will further the utility of these tumor models in preclinical and co-clinical drug development studies.publishersversionpublishe

A educação de jovens e adultos no município de Bagé/RS e as percepções dos atores sociais sobre a educação empreendedora no território

This paper presents an analysis of the perception of social actors regarding the Entrepreneurial Education proposed through the Young and Adult Education (EJA) in the territory of Bagé/RS. A qualitative research was carried out using data collection techniques such as bibliographic research and interviews, involving 59 subjects, among them students, technicians and managers of public schools that offer EJA in the municipality of Bagé/RS. The specialised bibliography reiterates that initiatives related to entrepreneurship, when inserted into the formal teaching and learning process, improve the quality of life, benefit less privileged communities and bring about processes of personal transformation. Although some students have expressed little motivation and/or interest in entrepreneurship, it is observed that, for the most part, those who had the opportunity of contact with Entrepreneurship Education showed satisfaction and recognition as to the importance of this subject. Moreover, the involvement, commitment and motivation of all the social actors analysed were decisive for the implementation of Entrepreneurship Education in the analysed territory.  Keywords: Entrepreneurship Education. Regional actors. Youth and Adult Education.Este artículo presenta un análisis de la percepción de los actores sociales sobre la Educación Emprendedora propuesta a través de la Enseñanza de Jóvenes y Adultos (EJA) en el territorio de Bagé/RS. Se realizó una investigación cualitativa con el uso de técnicas de recolección de datos como investigación bibliográfica y entrevistas, involucrando a 59 sujetos, entre ellos estudiantes, técnicos y gerentes de escuelas públicas que ofrecen EJA en el municipio de Bagé/RS. La bibliografía especializada refuerza que las iniciativas relacionadas con el emprendimiento, cuando se insertan en el proceso de enseñanza y aprendizaje formal amplían la calidad de vida, benefician a las comunidades menos privilegiadas y concretan procesos de transformación personal. Si bien algunos estudiantes han expresado poca motivación y/o interés por el emprendimiento, se observa que, en su mayoría, quienes tuvieron la oportunidad de contactar con Educación Emprendedora evidenciaron satisfacción y reconocimiento con respecto a la importancia de este tema. Además, la implicación, compromiso y motivación de todos los actores sociales analizados fue determinante para la efectividad de la Educación Emprendedora en el territorio analizado. Palabras clave: Educación emprendedora. Actores regionales. Educación de jóvenes y adultos.O presente artigo realiza uma análise sobre a percepção de atores sociais quanto à Educação Empreendedora proposta por meio do Ensino de Jovens e Adultos (EJA) no território de Bagé/RS. Realizou-se uma pesquisa de caráter qualitativo com a utilização de técnicas de coleta de dados como a pesquisa bibliográfica e entrevistas, envolvendo 59 sujeitos, entre eles alunos, técnicos e gestores de escolas públicas que oferecem o EJA no município de Bagé/RS. A bibliografia especializada reitera que iniciativas relacionadas ao empreendedorismo, quando inseridas no processo de ensino e aprendizagem formal, ampliam a qualidade de vida, beneficiam comunidades menos privilegiadas e concretizam processos de transformação pessoal. Embora alguns alunos tenham manifestado pouca motivação e/ou interesse em empreender, observa-se que, em sua maioria, aqueles que tiveram a oportunidade de contato com a Educação Empreendedora evidenciaram possuir satisfação e reconhecimento quanto à importância desta temática. Outrossim, o envolvimento, o comprometimento e a motivação de todos os atores sociais analisados foi determinante para a efetivação da Educação Empreendedora no território analisado.  Palavras-chave: Educação Empreendedora. Atores regionais. Educação de Jovens e Adultos

Patient-derived explants of colorectal cancer: histopathological and molecular analysis of long-term cultures

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Colorectal cancer (CRC) is one of the most common cancers worldwide. Although short-term cultures of tumour sections and xenotransplants have been used to determine drug efficacy, the results frequently fail to confer clinically useful information. Biomarker discovery has changed the paradigm for advanced CRC, though the presence of a biomarker does not necessarily translate into therapeutic success. To improve clinical outcomes, translational models predictive of drug response are needed. We describe a simple method for the fast establishment of CRC patient-derived explant (CRC-PDE) cultures from different carcinogenesis pathways, employing agitation-based platforms. A total of 26 CRC-PDE were established and a subset was evaluated for viability (n = 23), morphology and genetic key alterations (n = 21). CRC-PDE retained partial tumor glandular architecture and microenvironment features were partially lost over 4 weeks of culture. Key proteins (p53 and Mismatch repair) and oncogenic driver mutations of the original tumours were sustained throughout the culture. Drug challenge (n = 5) revealed differential drug response from distinct CRC-PDE cases. These findings suggest an adequate representation of the original tumour and highlight the importance of detailed model characterisation. The preservation of key aspects of the CRC microenvironment and genetics supports CRC-PDE potential applicability in pre- and co-clinical settings, as long as temporal dynamics are considered.This research was supported by AbbVie and by iNOVA4Health—UIDB/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia (FCT)/Ministério da Educação e Ciência, through national funds. TFM and SA were recipients of individual PhD fellowships funded by FCT (PD/BD/128377/2017 and PD/BD/105768/2014, respectively). CB acknowledges the support from “The Discoveries Centre for Regenerative and Precision Medicine” (European Commission Horizon 2020 Research and Innovation programme, under the Grant Agreement number 739572).info:eu-repo/semantics/publishedVersio

Exploring Metabolic Signatures of Ex Vivo Tumor Tissue Cultures for Prediction of Chemosensitivity in Ovarian Cancer

Predicting patient response to treatment and the onset of chemoresistance are still major challenges in oncology. Chemoresistance is deeply influenced by the complex cellular interactions occurring within the tumor microenvironment (TME), including metabolic crosstalk. We have previously shown that ex vivo tumor tissue cultures derived from ovarian carcinoma (OvC) resections retain the TME components for at least four weeks of culture and implemented assays for assessment of drug response. Here, we explored ex vivo patient-derived tumor tissue cultures to uncover metabolic signatures of chemosensitivity and/or resistance. Tissue cultures derived from nine OvC cases were challenged with carboplatin and paclitaxel, the standard-of-care chemotherapeutics, and the metabolic footprints were characterized by LC-MS. Partial least-squares discriminant analysis (PLS-DA) revealed metabolic signatures that discriminated high-responder from low-responder tissue cultures to ex vivo drug exposure. As a proof-of-concept, a set of potential metabolic biomarkers of drug response was identified based on the receiver operating characteristics (ROC) curve, comprising amino acids, fatty acids, pyrimidine, glutathione, and TCA cycle pathways. Overall, this work establishes an analytical and computational platform to explore metabolic features of the TME associated with response to treatment, which can leverage the discovery of biomarkers of drug response and resistance in OvC.publishersversionpublishe

Comparison of Probability Distribution Function in Determining Minimum Annual and Monthly Streamflow

Este trabalho buscou fornecer embasamento a estudos de disponibilidade hídrica para a bacia hidrográfica do Arroio Belo, localizado no município de Caxias do Sul/RS. Para tanto, objetivou-se analisar a aplicação das funções de distribuição de probabilidade teórica Weibull, Normal, Log-Normal, Gumbel (mínimos), Log-Pearson e Pearson a dados de vazões mínimas de sete dias consecutivos. A análise teve dois enfoques: aplicação em dados anuais e, em seguida em dados mensais, considerando a sazonalidade. Para verificar a aderência das probabilidades estimadas às frequências observadas, aplicaram-se três testes: Kolmogorov-Smirnov, Anderson-Darling e Qui-Quadrado. Os resultados obtidos comprovam que a distribuição Log-Pearson III demonstra maior precisão na representação dos dados anuais da série histórica e alcança o melhor ajuste do valor da vazão mínima. Por outro lado, a análise em dados mensais, indicou a utilização da distribuição Pearson III, a qual apresentou maior adequabilidade aos dados de vazão mínima.  http://dx.doi.org/10.18226/23185279.v3iss2p42In this study it was aimed to provide the foundation studies of water availability in the Arroio Belo basin, in Caxias do Sul/RS. Therefore, this study aimed to analyze the application of Weibull, Normal, LogNormal, Gumbel (minimum), LogPearson and Pearson theoretical probability functions to data of minimum streamflows for seven consecutive days of the basin. The analysis had two approaches: application in annual data, and then on monthly data, considering seasonality. To verify the adherence to the estimated probabilities of observed frequencies, we applied three tests: Kolmogorov-Smirnov, Anderson-Darling and Chi-Squared. The results show that the Log-Pearson III distribution shows greater accuracy in representing the annual data of the series and reach the best fit of the minimum streamflow. The monthly data analysis indicated the use of the distribution Pearson III, which showed higher suitability to the minimum streamflow data.  http://dx.doi.org/10.18226/23185279.v3iss2p4

Anais do 1º Encontro de Iniciação Científica e de Extensão da Unila: "Conhecer e Transformar"

Anais do I Encontro de Iniciação Científica e de Extensão da Unila. Unila-Centro, Foz do Iguaçu, Estado do Paraná, 04 e 05 de junho de 2012A indissocialibidade entre as ações de ensino, pesquisa e extensão, cuja unidade caracteriza o processo pedagógico no ensino superior tem, neste evento, a manifestação da sua aplicabilidade. Os planos de trabalho e projetos de estudantes e orientadores de iniciação científica e extensão demarcam os processos formadores da capacidade de pensamento crítico dos futuros profissionais egressos de uma universidade inovadora. Neste processo, a iniciação científica tem a missão de aperfeiçoar a formação acadêmica e profissional de estudantes de graduação, que são introduzidos nos diferentes campos do Saber pelas disciplinas e projetos de pesquisa docente para serem capacitados a refletir sobre limitações das sociedades, formular e testar hipóteses, resolver problemas e situações colocadas tanto pela simples curiosidade humana de conhecer quanto pela necessidade social de transformar. Constitui-se na formulação de questionamentos, na aprendizagem da elaboração do objeto de pesquisa, escolha dos referenciais epistemológicos e metodológicos, busca de informações, sistematização da argumentação e produção de conhecimento. A extensão é, por natureza, de vocação transformadora da realidade social, cultural e ambiental. Demanda uma ação de pesquisa, que pode ser chamada de pesquisa-ação ou de observação. Envolve a todos, comunidade universitária e comunidade onde está inserida a Universidade, num processo de troca de saberes. Os saberes e conhecimentos adquiridos, bem como as tecnologias produzidas, fomentam resultados coletivos sempre e quando são transferidos sob os princípios da responsabilidade, cooperação, solidariedade, racionalidade e da inclusão. Espera-se que cada estudante desenvolva atitudes críticas e habilidades de pesquisador, tais como dedicação, criatividade, honestidade, ética e compromisso com a transformação da realidade. O I Encontro de Iniciação Científica e de Extensão da Unila colabora para a avaliação e a exposição pública dos resultados dos projetos. Será realizada uma conferência de abertura composta pelo Magnífico Reitor Pro tempore da Unila, Hélgio Trindade, pelo Pró-Reitor de Pesquisa e Pós-graduação, Andrea Ciacchi, pela Pró-Reitora de Extensão, Luisa Maria de Moura e Silva e pelo pesquisador Flávio Bortolozzi, para expor ao público o tema “Pesquisa no Meio Acadêmico e suas Oportunidades”. O evento contará ainda com a palestra da pesquisadora Laura Tavares Ribeiro Soares com o título “A Pesquisa na Extensão”.Universidade Federal da Integração Latino-Americana (UNILA

Critical steps in clinical shotgun metagenomics for the concomitant detection and typing of microbial pathogens

High throughput sequencing has been proposed as a one-stop solution for diagnostics and molecular typing directly from patient samples, allowing timely and appropriate implementation of measures for treatment, infection prevention and control. However, it is unclear how the variety of available methods impacts the end results. We applied shotgun metagenomics on diverse types of patient samples using three different methods to deplete human DNA prior to DNA extraction. Libraries were prepared and sequenced with Illumina chemistry. Data was analyzed using methods likely to be available in clinical microbiology laboratories using genomics. The results of microbial identification were compared to standard culture-based microbiological methods. On average, 75% of the reads corresponded to human DNA, being a major determinant in the analysis outcome. None of the kits was clearly superior suggesting that the initial ratio between host and microbial DNA or other sample characteristics were the major determinants of the proportion of microbial reads. Most pathogens identified by culture were also identified through metagenomics, but substantial differences were noted between the taxonomic classification tools. In two cases the high number of human reads resulted in insufficient sequencing depth of bacterial DNA for identification. In three samples, we could infer the probable multilocus sequence type of the most abundant species. The tools and databases used for taxonomic classification and antimicrobial resistance identification had a key impact on the results, recommending that efforts need to be aimed at standardization of the analysis methods if metagenomics is to be used routinely in clinical microbiology

Author Correction: Critical steps in clinical shotgun metagenomics for the concomitant detection and typing of microbial pathogens (vol 8, 13767, 2018)

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper