Proizvodnja α-amilaze iz Penicillium chrysogenum fermentacijom poljoprivrednih nusproizvoda

Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from P. chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55 % for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of α-amylase were: >1 mm, 20 %; >1 mm, 20 %; 1 mm, 20 % and >1 mm, 30 % for CL, WS, WB and RS, respectively. WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively.Komušina, slama od raži, slama od pšenice i pšenične posije upotrijebljeni su kao supstrat za fermentaciju na čvrstoj podlozi pri proizvodnji α-amilaze iz Penicillium chrysogenum. Ispitan je utjecaj vlage, veličine čestica i koncentracije inokuluma na sintezu enzima iz Penicillium chrysogenum. Optimalna količina vlage u supstratima bila je 75 % za komušinu, 65 % za slamu od pšenice i pšenične posije, te 55 % za slamu od raži. Veličina čestica i koncentracija inokuluma bili su >1 mm odnosno 20 % za komušinu i slamu od pšenice, 1 mm odnosno 20 % za pšenične posije, te >1 mm odnosno 30 % za slamu od raži. Najveća proizvodnja enzima od 160 U/mL postignuta je upotrebom pšeničnih posija u optimalnim uvjetima. Uporabom komušine aktivnost enzima bila je 28, slame od raži 49, a slame od pšenice 45 U/mL

Amylolitic Activities of Different Fungi Species in the Screening Medium Containing Different Raw Starch

DergiPark: 246086trakyafbdThirty-nine fungal species were screened for the production of extracellular amylase hydrolyzing raw starch using a plate culture method. Czapek-Dox Agar containing different raw starch (corn, wheat, potato and rice) was used as culture medium for screening. Among these, thirteen, twelve, seven and five fungi showed higher amylolytic activity on solid medium containing raw wheat starch, raw rice starch, raw potato starch and raw corn starch, respectively. Two fungi did not show any amylolytic activityOtuz dokuz fungus türü ham nişastayı hidroliz eden amilazları üretebilmeleri açısından Petri kültür metodu ile tarandı. Karbon kaynağı olarak farklı ham nişastaları içeren Czapek-Dox Agar ham nişastayı hidroliz eden amilaz üreticilerinin taranması için kullanıldı. Bunlar arasında on üç, on iki, yedi ve beş fungus sırası ile ham buğday, pirinç, patates ve mısır nişastalarını içeren besiyerlerinde en yüksek amilolitik aktiviteyi gösterdi. İki fungus ham nişasta içeren besiyerlerinde amilolitik aktivite göstermed

Immobilization of B. amyloliquefaciens α-amylase and comparison of some of its enzymatic properties with the free form

The enzyme alpha-Amylase from Bacillus amyloliquefaciens was entrapped by drop-wise addition of an aqueous mixture of sodium alginate in the solution of a Ca2+ salt. Effects of immobilization conditions were investigated. Optimum alginate and CaCl2 concentrations were found to be 2% and 5% (w/v), respectively. The immobilization yield was 89%. Amylase activity increased with increasing enzyme loading on the beads. The best size and amount of beads for achieving enzyme activity were found to be 3 mm and 0.4 g, respectively. When coated with silicate, amylase-entrapped beads retained the highest catalytic activity. The highest operational stability was six reuses with 51% residual activity. Some properties of immobilized enzyme were determined, and compared with those of free enzyme. Product profile of the various raw starches has been determined by thin layer chromatography

Production of α-Amylase from Penicillium chrysogenum under Solid-State Fermentation by Using Some Agricultural By-Products

Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from P. chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55 % for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of α-amylase were: >1 mm, 20 %; >1 mm, 20 %; 1 mm, 20 % and >1 mm, 30 % for CL, WS, WB and RS, respectively. WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively

AGARICUS CAMPESTRIS, PLEUROTUS ERYNGII VE LACTARIUS DELICIOSUS MANTARLARININ ANTİOKSİDAN ÖZELLİKLERİNİN BELİRLENMESİ

DergiPark: 434685klujesAgaricus campestris, Pleurotus eryngii and Lactariusdeliciosus are species of fungi that naturally grow in the Thrace region. Mushroomshave been used for food and medical purposes for centuries. In this study, theDPPH scavenging activity, the total phenolic substance contents and thereducing power capacities on the methanol and acetone extracts of the mushroomsamples were studied. When the DPPH radical scavenging activities are takeninto account, the activity is increased depending on the concentration. Thehighest scavenging activity was found in the acetone extract of P. eryngii with 39.86 ± 2.07 %. It wasdetermined that the total phenolic substances in acetone and methanol extractsof mushroom samples varied between 23,25 ± 0,35-28 ± 0,7 ?g GAE / mg extract.The sample showing the highest reducing power capacity was an acetone extractof P. eryngii with a concentration of0,400 ± 0,021 ?g/mL at a concentration of 1000 ?g/mL followed by the methanolextract of P. eryngii (0.259 ± 0.030)and the acetone extract of L. deliciosus(0.143 ± 0.010).Agaricuscampestris , Pleurotus eryngii ve Lactarius deliciosusTrakya bölgesinde doğal olarak yetişebilen mantar türleridir. Mantarlaryüzyıllardır besin kaynağı olarak ve tıbbi amaçla kullanılmaktadırlar. Buçalışmada mantar örneklerinin metanol ve aseton ekstraktlarının DPPH radikalinisüpürme etkileri, toplam fenolik madde miktarları ve indirgeme gücükapasiteleri çalışılmıştır. DPPH radikalini süpürme etkileri göz önündebulundurulduğunda konsantrasyona bağlı olarak aktiviteleri artmıştır. En yükseksüpürme etkisi % 39,86± 2,07 ile P. eryngii’nin aseton ekstraktında görülmüştür. Mantar örneklerininaseton ve metanol ekstraktlarının toplam fenolik madde miktarlarının23,25±0,35-28±0,7 µg GAE/mg ekstre arasında değiştiği belirlenmiştir. En yüksekindirgeme gücü kapasitesi gösteren örnek 1000 µg/mL’likkonsantrasyonda 0,400±0,021 µg\mL ile P. eryngii’ninaseton ekstraktı olmuştur, bu örneği P.eryngii’nin metanol ekstraktı  (0,259±0,030)ve L.deliciosus’un aseton ekstraktı (0,134±0,010) takip etmektedir. 

Determination of the effects of initial glucose on the production of alpha-amylase from Penicillium sp under solid-state and submerged fermentation

The effects of catabolite repression of initial glucose on the synthesis of alpha-amylase from Penicillium chrysogenum and Penicillium griseofulvum were investigated under solid-state fermentation (SSF) and submerged fermentation (SmF) systems. The results obtained from either fermentation were compared with each other. In the SmF system, initial glucose concentration above 10 mg/mL completely repressed the production of alpha-amylase from P. chrysogenum and P. griseofulvum. However, the repression in the SSF system was not complete, even when the glucose level was raised to 160 mg/g.WOS:0003380158000142-s2.0-84905919100PubMed: 2601949