Dairy Nasal Lavage and Exposure Data

Data set includes nasal pro and anti-inflammatory cytokine concentrations and dust and endotoxin exposure concentrations for dairy workers at a north Texas dairy taken in May of 2017.Livestock workers experience an increased burden of bioaerosol-induced respiratory disease including high prevalence of rhinosinusitis. Dairy operations generate bioaerosols spanning the inhalable size fraction (0-100 μm) containing bacterial constituents such as endotoxin. Particles with an aerodynamic diameter between 10-100 μm are known to deposit in the nasopharyngeal region and likely affect the upper respiratory tract. We evaluated the effectiveness of a hypertonic saline nasal lavage in reducing inflammatory responses in dairy workers from a high-volume dairy operation. Inhalable personal breathing zone samples and pre-/post-shift nasal lavage samples from each participant over five consecutive days were collected. The treatment group (n=5) received hypertonic saline while the control group (n=5) received normotonic saline. Personal breathing zone samples were analyzed for particulate concentrations and endotoxin using gravimetric and enzymatic methods, respectively. Pro- and anti-inflammatory cytokines (i.e., IL-8, IL-10, and TNF-) were measured from nasal lavage samples using a multiplex assay. Inhalable dust concentrations ranged from 0.15 to 1.9 mg/m3. Concentrations of both pro- and anti-inflammatory cytokines, specifically IL-6, IL-8, and IL-10, were significantly higher in the treatment group compared to the control group (p < 0.02, p < 0.04, and p < 0.01 respectively). Further analysis of IL-10 anti-inflammatory indicates a positive association between hypertonic saline administration and IL-10 production. This pilot study demonstrates that hypertonic saline nasal lavages were successful in upregulating anti-inflammatory cytokines to support larger interventional studies

Do grasshoppers prefer one species of lentil over another?

Non-Peer ReviewedSevere outbreaks of grasshoppers cause yield losses in many economically important crops. Lentil (Lens culinaris) is particularly susceptible to grasshopper damage. The weather patterns of 2001-2002 in most of the lentil production regions of Saskatchewan contributed to the outbreak in grasshopper populations resulting in major damage to the lentil crop. Grasshoppers preferentially target the flowers and young pods of lentil plants. Lentil plants, due to their indeterminate growth habit, will re-grow if moisture is available, leading to development of new pods, resulting in delayed maturity and further grasshopper damage. Pods that are damaged or not consumed entirely may shatter or not fill properly. Grasshoppers are known to have very specific feeding habits and may show a range of preference from one plant species to another based on taste or texture. One of the seven known species of lentil has hairy pods. Grasshopper feeding preferences were examined on five lentil species, including the cultivated lentil. The purpose was to determine if grasshoppers prefer to feed on the cultivated lentil compared to four wild species. If they do, it may be possible in future to breed lentil varieties that are less damaged by grasshoppers by using the wild lentil species in the breeding program

Implementation Research on Community Health Workers’ Provision of Maternal and Child Health Services in Rural Liberia

OBJECTIVE: To assess changes in the use of essential maternal and child health services in Konobo, Liberia, after implementation of an enhanced community health worker (CHW) programme. METHODS: The Liberian Ministry of Health partnered with Last Mile Health, a nongovernmental organization, to implement a pilot CHW programme with enhanced recruitment, training, supervision and compensation. To assess changes in maternal and child health-care use, we conducted repeated cross-sectional cluster surveys before (2012) and after (2015) programme implementation. FINDINGS: Between 2012 and 2015, 54 CHWs, seven peer supervisors and three clinical supervisors were trained to serve a population of 12 127 people in 44 communities. The regression-adjusted percentage of children receiving care from formal care providers increased by 60.1 (95% confidence interval, CI: 51.6 to 68.7) percentage points for diarrhoea, by 30.6 (95% CI: 20.5 to 40.7) for fever and by 51.2 (95% CI: 37.9 to 64.5) for acute respiratory infection. Facility-based delivery increased by 28.2 points (95% CI: 20.3 to 36.1). Facility-based delivery and formal sector care for acute respiratory infection and diarrhoea increased more in agricultural than gold-mining communities. Receipt of one-or-more antenatal care sessions at a health facility and postnatal care within 24 hours of delivery did not change significantly. CONCLUSION: We identified significant increases in uptake of child and maternal health-care services from formal providers during the pilot CHW programme in remote rural Liberia. Clinic-based services, such as postnatal care, and services in specific settings, such as mining areas, require additional interventions to achieve optimal outcomes

In-situ characterization of the Hamamatsu R5912-HQE photomultiplier tubes used in the DEAP-3600 experiment

The Hamamatsu R5912-HQE photomultiplier-tube (PMT) is a novel high-quantum efficiency PMT. It is currently used in the DEAP-3600 dark matter detector and is of significant interest for future dark matter and neutrino experiments where high signal yields are needed. We report on the methods developed for in-situ characterization and monitoring of DEAP's 255 R5912-HQE PMTs. This includes a detailed discussion of typical measured single-photoelectron charge distributions, correlated noise (afterpulsing), dark noise, double, and late pulsing characteristics. The characterization is performed during the detector commissioning phase using laser light injected through a light diffusing sphere and during normal detector operation using LED light injected through optical fibres

A drowned Mesolithic shell midden complex at Hjarnø Vesterhoved,Denmark and its wider significance

Anthropogenic shell accumulations (shell middens), often of great size, occur in their tens of thousands around the world’s coastlines. They mostly date from the Mid-Holocene onwards and are frequently taken as symptomatic of a Postglacial ‘revolution’ involving world-wide population growth and intensification in exploitation of marine resources. However, the comparative rarity of earlier deposits may have as much to do with Postglacial sea-level rise and the loss of evidence from earlier palaeoshorelines as with genuine socio-economic trends. Here we investigate the underwater Mesolithic (Ertebølle) shellmidden of Hjarnø Vesterhoved in Denmark, one of the first underwater shell middens to be systematically verified as an anthropogenic shell deposit in a region world-famous for its many hundreds of Ertebølle shell mounds on the present shoreline. We show how a combination of geophysical survey, coring, excavation, stratigraphic interpretation and macroscopic analysis of midden contents can be used to identify underwater deposits, to unravel their taphonomic and post-depositional history in relation to surrounding sediments, and to distinguish between cultural and natural agencies of shell accumulation and deformation. We demonstrate the presence of an intact underwater shell-midden deposit dated at 5400–5100 cal BC, one of the earliest in Denmark. We demonstrate the usefulness of such material in giving new information about early coastal subsistence economies and greater precision to the measurement of palaeosea levels. We discuss the implications of our results for an improved understanding of the Mesolithic record in Denmark and of biases in the archaeological record of Late Pleistocene and Early-to-Mid-Holocene coastal contexts. We emphasise the importance of researching more fully the geomorphological and taphonomic processes that affect the accumulation, destruction, burial, preservation and visibility of underwater archaeological deposits, the need to extend underwater investigations more widely and to more deeply submerged palaeoshorelines, and the combination of methods required to advance such investigations

ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery

Funding: Royal Society for a University Research Fellowship; Wellcome Multi-User Equipment Grant (099149/Z/12/Z) (JEL).Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. Previously, we proposed a Brownian ratchet model for transport, whereby the free energy of ATP-turnover favours the directional diffusion of the polypeptide [Allen et al. eLife 2016]. Here, we show that ATP enhances this process by modulating secondary structure formation within the translocating protein. A combination of molecular simulation with hydrogen-deuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. This ability to exploit structure within a pre-protein is an unexplored area of protein transport, which may apply to other protein transporters, such as those of the endoplasmic reticulum and mitochondria.Publisher PDFPeer reviewe

Electromagnetic backgrounds and potassium-42 activity in the DEAP-3600 dark matter detector

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Design and construction of the DEAP-3600 dark matter detector

The Dark matter Experiment using Argon Pulse-shape discrimination (DEAP) has been designed for a direct detection search for particle dark matter using a single-phase liquid argon target. The projected cross section sensitivity for DEAP-3600 to the spin-independent scattering of Weakly Interacting Massive Particles (WIMPs) on nucleons is 10 cm for a 100 GeV/c WIMP mass with a fiducial exposure of 3 tonne-years. This paper describes the physical properties and construction of the DEAP-3600 detector. −46 2

Search for dark matter with a 231-day exposure of liquid argon using DEAP-3600 at SNOLAB

DEAP-3600 is a single-phase liquid argon (LAr) direct-detection dark matter experiment, operating 2 km underground at SNOLAB (Sudbury, Canada). The detector consists of 3279 kg of LAr contained in a spherical acrylic vessel. This paper reports on the analysis of a 758  tonne⋅day exposure taken over a period of 231 live-days during the first year of operation. No candidate signal events are observed in the WIMP-search region of interest, which results in the leading limit on the WIMP-nucleon spin-independent cross section on a LAr target of 3.9×10−45  cm2 (1.5×10−44  cm2) for a 100  GeV/c2 (1  TeV/c2) WIMP mass at 90% C.L. In addition to a detailed background model, this analysis demonstrates the best pulse-shape discrimination in LAr at threshold, employs a Bayesian photoelectron-counting technique to improve the energy resolution and discrimination efficiency, and utilizes two position reconstruction algorithms based on the charge and photon detection time distributions observed in each photomultiplier tube