Results of the quantitative analysis of GABA immunoreactivity.

<p>Mean values are shown with ranges in brackets (n = 4).</p

GABA and glycine immunoreactivity in a 0.5 µm thick transverse section of the mouse dorsal horn.

<p><b>a</b> shows the whole mediolateral extent of the dorsal horn stained for GABA, while <b>b</b> and <b>c</b> show the medial part of the dorsal horn at higher magnification, in the same section and in a section reacted for glycine, respectively. Many GABA-immunoreactive cell bodies are scattered throughout the dorsal horn, and although these vary in intensity, they are clearly darker than the immunonegative cells, some of which are marked with asterisks in <b>b</b>. The dark staining in the neuropil represents GABAergic axons and dendrites. Dashed lines show the ventral borders of laminae I, II and III. <b>b</b> and <b>c</b> are serial semithin sections, so the same cells are visible. A few cells with relatively strong glycine immunoreactivity are visible (some marked with arrows) and these are also GABA-immunoreactive. In addition, there are cells that are GABA- but not glycine-immunoreactive (two marked with arrowheads). Scale bars  = 100 µm.</p

Distribution of sst_2A immunoreactivity in the mouse dorsal horn.

<p><b>a</b>: There is a dense band of immunostaining in laminae I and II, with much lower levels elsewhere in the grey matter. Immunoreactive cells (some marked with arrows) are scattered throughout laminae I and II, and present at much lower density in deeper laminae. The solid line shows the grey-white matter boundary and the dashed line the border between laminae II and III. <b>b–f</b>: The relationship between sst_2A (green), PKCγ (magenta) and the appearance of the dorsal horn with dark-field illumination (DF). Note that although scattered PKCγ cells are present in lamina III, the ventral border of the PKCγ plexus (arrowheads) is at the same level as the ventral edge of sst_2A staining, and that this corresponds to the lamina II/III border as seen with dark-field microscopy. All confocal images are from single optical sections. Scale bar  = 100 µm.</p

NK1r and sst2A in superficial dorsal horn.

<p>Confocal image showing a single optical section through part of laminae I and IIo, scanned to reveal NK1r (green) and sst_2A (magenta). <b>a</b>: Two NK1r-immunoreactive cell bodies (arrows) are visible in lamina I, surrounded by a plexus of labelled dendrites. <b>b</b>: Several sst_2A-immunoreactive cells are visible in the same field (two shown with arrowheads). <b>c</b>: The merged image shows that none of these cells is labelled with both antibodies. Scale bar  = 50 µm.</p

Proportions of neurons in laminae I-III that were sst_2A- or NK1r-immunoreactive and numbers of neurons in each lamina.

<p>Mean values are shown with ranges in brackets (n = 4). The estimated total number of neurons in L4 is based on the average length of the segment (1.45 mm) in these 4 mice.</p

Primary antibodies used in this study.

*<p>dilutions used for post-embedding method</p

Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn-7

<p><b>Copyright information:</b></p><p>Taken from "Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn"</p><p>http://www.molecularpain.com/content/4/1/5</p><p>Molecular Pain 2008;4():5-5.</p><p>Published online 23 Jan 2008</p><p>PMCID:PMC2248168.</p><p></p>ina IIi. These images were projected from 2 optical sections at 0.3 μm z-separation. Scale bar = 5 μm

Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn-5

<p><b>Copyright information:</b></p><p>Taken from "Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn"</p><p>http://www.molecularpain.com/content/4/1/5</p><p>Molecular Pain 2008;4():5-5.</p><p>Published online 23 Jan 2008</p><p>PMCID:PMC2248168.</p><p></p>ice in which the genes coding for GluR1, GluR2 and GluR3 (g, and ) had been knocked out. Each row shows staining in a different mouse, and in each case staining for GluR1 (red) is shown in the left column, followed by staining for GluR2 (blue) and GluR3 (green), with a merged image in the right column. Note the lack of staining for the corresponding subunit in each of the knock-out mice. Images show projections of 8 optical sections at 0.3 μm z-separation. Scale bar = 5 μm

Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn-3

<p><b>Copyright information:</b></p><p>Taken from "Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn"</p><p>http://www.molecularpain.com/content/4/1/5</p><p>Molecular Pain 2008;4():5-5.</p><p>Published online 23 Jan 2008</p><p>PMCID:PMC2248168.</p><p></p> Note that in this field the two types of immunoreactivity are contained in different puncta, with no co-localisation. This is a projection of 16 optical sections at 0.3 μm z-spacing. Scale bar = 5 μm

Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn-9

<p><b>Copyright information:</b></p><p>Taken from "Expression of AMPA receptor subunits at synapses in laminae I–III of the rodent spinal dorsal horn"</p><p>http://www.molecularpain.com/content/4/1/5</p><p>Molecular Pain 2008;4():5-5.</p><p>Published online 23 Jan 2008</p><p>PMCID:PMC2248168.</p><p></p>e labelled with the GluR4-C antibody (two marked with arrows). The GluR4-N antibody stains these strongly, but also labels several other puncta more weakly (two shown with arrowheads). All of the puncta labelled by each GluR4 antibody are GluR2-immunoreactive. Images were obtained from 4 optical spacing at 0.3 μm. Scale bar = 10 μm